链霉菌TOR3209及其挥发性有机化合物提高烟草抗木贼镰孢的能力

doi:10.3864/j.issn.0578-1752.2025.11.007

Abstract

Abstract:

【Objective】The objective of this study is to explore the effect of Streptomyces TOR3209 and its volatile organic compounds (VOCs) on tobacco disease resistance, clarify the direct and indirect mechanism of the strain and its VOCs to improve tobacco disease resistance, and to provide a theoretical basis for the biocontrol application of TOR3209 and its VOCs.【Method】Streptomyces TOR3209 and Fusarium equiseti were selected as research materials, and the inhibition ability of TOR3209 and its VOCs against F. equiseti was evaluated by using plate confrontation method and growth rate method. Tobacco was inoculated with different concentrations of TOR3209 suspension (1.0×10¹, 1.0×10³, 1.0×10⁵, 1.0×10⁷ CFU/mL) and co-cultured with VOCs produced by TOR3209. The effects of the strain and its VOCs on tobacco resistance to F. equiseti were tested. The transcriptome sequencing of tobacco was performed to analyze the differential gene expression. Solid phase microextraction (SPME) method was used to identify the VOCs produced by TOR3209, and a compound with strong specificity (heptadecane) was selected to verify the resistance of tobacco to F. equisetaria. Different concentrations of heptadecane (1 mol·kg^-1, 1 mmol·kg^-1, 1 μmol·kg^-1, 0.001 μmol·kg^-1) were added as treatment groups to verify the effect of heptadecane on tobacco.【Result】Streptomyces TOR3209 and its VOCs had antagonistic effects on F. equiseti, with inhibition rates of 56.9% and 60.0%, respectively. Compared with the control, the severity of tobacco disease was significantly reduced under different concentrations of TOR3209 suspension (P<0.01), and the treatment with the concentration of 1.0×10¹ CFU/mL had the best disease resistance effect. Both the retained strain treatment group and the removed strain treatment group could improve the disease resistance of tobacco, the control effect was 85.7% and 72.5%, respectively. The main way was to induce tobacco systemic resistance during the action of TOR3209 VOCs. Transcriptome analysis of tobacco showed that the VOCs of TOR3209 induced up-regulation of several genes in tobacco, and many of the genes were related to the improvement of plant disease resistance. Among the VOCs produced by TOR3209, several compounds were identified, among which heptadecane could improve the resistance of tobacco to F. equiseti infection after co-culture with tobacco, and the treatment group with a mass concentration of 1 mmol·kg^-1 had a significant effect.【Conclusion】Streptomyces TOR3209 and its VOCs mainly improve the resistance of tobacco by inducing systemic resistance, and the VOCs of TOR3209 can regulate the gene expression of tobacco.

Key words: Streptomyces TOR3209, tobacco, Fusarium equiseti, disease resistance, antagonism, biological control

ZHAO LinLin, HE YuXi, PENG JieLi, WANG Xu, MA Jia, ZHANG XiuMin, HU Dong. Streptomyces TOR3209 and Its Volatile Organic Compounds Enhance Tobacco Resistance to Fusarium equiseti[J].Scientia Agricultura Sinica, 2025, 58(11): 2162-2175.

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Figures/Tables 13

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Table 1

Important differentially expressed genes in tobacco plants inoculated with TOR3209 (part)"

基因号Gene ID	log₂ fold change	基因描述Gene description
上调基因Up-regulated gene
Niben101Scf00332g06017	6.04227246102319	杂交信号转导组氨酸激酶I Hybrid signal transduction histidine kinase I
Niben101Scf05631g00014	5.74326370463322	非特异性脂质转移蛋白2 Non-specific lipid-transfer protein 2
Niben101Scf01362g04002	4.77714497698617	2-氨基乙硫醇双加氧酶样蛋白2-aminoethanethiol dioxygenase-like protein
下调基因Down-regulated gene
Niben101Scf02911g03016	-5.78546446761017	未知功能蛋白Protein of unknown function (DUF506), LENGTH=295
Niben101Scf00073g03014	-5.45982567636163	受体样蛋白激酶Receptor-like protein kinase
Niben101Scf01428g04013	-5.26257570331272	乙烯反应性转录因子14 Ethylene-responsive transcription factor 14
Niben101Scf16511g00010	-5.20322117747684	类萜合酶18 Terpenoid synthase 18
Niben101Scf04932g00014	-5.16902219728486	未知蛋白Unknown protein
Niben101Scf02917g00002	-5.09895124325375	TGACG-序列特异性DNA结合蛋白TGA-2.1 TGACG-sequence-specific DNA-binding protein TGA-2.1
Niben101Scf00448g07004	-4.92380517686696	丝氨酸/苏氨酸蛋白激酶Serine/threonine-protein kinase 3
Niben101Scf01911g05005	-4.89265174714343	碳酸酐酶Carbonic anhydrase
Niben101Scf03309g04008	-4.82172737214981	磷脂酶A1-II Phospholipase A1-II
Niben101Scf06662g02015	-4.62438225777014	基质金属蛋白酶Matrix metalloproteinase, LENGTH=378
Niben101Scf01185g00004	-4.6100638982765	蛋白磷酸酶2C Protein phosphatase 2C
Niben101Scf00073g03013	-4.56247469616356	受体样蛋白激酶Receptor-like protein kinase
Niben101Scf24315g00001	-4.55766816248908	嘌呤渗透酶3 Purine permease 3
Niben101Scf01899g06004	-4.51115797208459	脱氢酶/还原酶SDR家族成员Dehydrogenase/reductase SDR family member 4
Niben101Scf10524g02009	-4.48990826668509	结节蛋白MtN21 /EamA样转运蛋白家族蛋白 Nodulin MtN21 /EamA-like transporter family protein, LENGTH=365
Niben101Scf02083g00003	-4.47336269506046	CRN家族蛋白[疫霉T30-4] crinkler (CRN) family protein [Phytophthora infestans T30-4], gb\|EEY64563.1\|
Niben101Scf01789g03005	-4.40149896063059	[疫霉T30-4] [Phytophthora infestans T30-4]
Niben101Scf07663g00007	-4.35828100466665	保守假设蛋白[蓖麻] Conserved hypothetical protein [Ricinus communis], gb\|EEF51416.1\|
Niben101Scf00192g01021	-4.21433821585193	细胞周期蛋白-D3-1 Cyclin-D3-1
Niben101Scf04410g03007	-4.2077309454339	D-氨基酰-tRNA脱酰基酶D-aminoacyl-tRNA deacylase
Niben101Scf00270g14005	-4.09796972283243	未知功能蛋白Protein of unknown function (DUF1264), LENGTH=241
Niben101Scf02391g00013	-4.05678468762749	丝氨酸羧肽酶样31 Serine carboxypeptidase-like 31
Niben101Scf03473g01018	-4.05495408176547	GRAS家族转录因子GRAS family transcription factor, LENGTH=490

Table 1

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Streptomyces TOR3209 and Its Volatile Organic Compounds Enhance Tobacco Resistance to Fusarium equiseti

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