Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (6): 1189-1198.doi: 10.3864/j.issn.0578-1752.2022.06.011

• HORTICULTURE • Previous Articles     Next Articles

Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings

DONG SangJie1(),JIANG XiaoChun1,WANG LingYu1,LIN Rui1,QI ZhenYu2,YU JingQuan1,ZHOU YanHong1()   

  1. 1Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University/State Agriculture Ministry Key Laboratory of Horticultural Plant Growth and Development, Hangzhou 310058
    2Agricultural Experimental Station, Zhejiang University, Hangzhou 310058
  • Received:2021-06-23 Accepted:2021-09-09 Online:2022-03-16 Published:2022-03-25
  • Contact: YanHong ZHOU E-mail:dongsangjie@zju.edu.cn;yanhongzhou@zju.edu.cn

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Abstract:

【Objective】This study analyzed the effects of supplementary far-red light (FR) on the growth and abiotic stress tolerance of pepper seedlings, aiming to provide a theoretical basis on precise light environments for cultivating high quality vegetable seedlings.【Method】In this study, Bola Hongshuai pepper cultivar was used as the research material. The 7-day-old seedlings were cultivated under two LED light environments, including the control spectrum (NL; R/B = 3/1, 150 μmol∙m -2∙s-1 PPFD) and the NL with an extra 10 μmol∙m -2∙s-1far-red light (6% FR), 20 μmol∙m -2∙s-1far-red light (13% FR), and 30 μmol∙m -2∙s-1far-red light (20% FR). Chilling and drought were imposed when the seedlings were 21 days old. Biomass, resistance-related gene expression, antioxidant enzyme activity, hormone content, chlorophyll fluorescence parameters, and leaf relative electrolyte leakage (REL) were analyzed to explore the effects of supplemental FR on growth and abiotic stress tolerance of pepper seedlings. 【Result】 Compared with the control, the supplementation of 6% FR was beneficial to increase the height, stem thickness, dry weight, fresh weight and seedling indexes of pepper seedlings. Moreover, the supplementation of 6% FR significantly increased the expression of the cold response gene CBF1 and antioxidant enzyme-related genes, such as Cu/Zn-SOD, GR, APX, CAT and DHAR under chilling stress. The activity of SOD, APX, DHAR, CAT and GR as well as the ABA content of pepper seedlings under low temperature increased by 25.2%, 53.6%, 55.8%, 72.7%, 33.4% and 69.5%, respectively, following the treatment with supplemental FR. The PSII maximum photochemical efficiency (Fv/Fm) of pepper leaves after supplementation of 6% FR under low temperature stress significantly increased compared with the control, while REL decreased obviously, indicating that supplementation of 6% FR alleviated the low temperature-induced PSII photoinhibition and damage in leaves and enhanced the cold tolerance of pepper seedlings. Under the drought stress condition, compared with the control, the supplementation of 6% FR improved the activity of SOD, GR, APX, CAT and DHAR by 13.7%, 38.0%, 37.2%, 27.6% and 23.7%, respectively. The ABA content and PSII actual photochemical efficiency (ΦPSII) were both increased while REL was decreased significantly. The results showed that the supplementation of 6% FR reduced the degree of PSII photoinhibition and membrane lipid peroxidation caused by drought stress, and improved the drought tolerance of pepper seedlings.【Conclusion】The study found that supplementation of 6% FR could not only improve the quality of pepper seedlings, but also enhance the resistance of pepper seedlings to low temperature and drought stresses by improving antioxidant defense and ABA homeostasis.

Key words: pepper (Capsicum annuum L.), far-red light, seedling index, low temperature stress, drought stress

Fig. 1

Relative spectral curve of the LED lamp used in the test"

Table 1

Primers used for real-time quantitative PCR"

基因名称 Gene name 正向引物（5′-3′）Forward primer 反向引物（5′-3′）Reverse primer
UBI-3 TGTCCATCTGCTCTCTGTTG CACCCCAAGCACAATAAGAC
CBF1 CCGGCTTCCTCTGACACTAA GTAATCCCGGCATGCAGAAG
Cu/Zu-SOD ATGGAGATGCTCCAACCACA CCAAGATCACCAGCATGACG
APX TCTGTGAAGCGGTGAAGTCT GTGGCACACCTTGTTTAGCA
CAT AAGCAGGCTGGGGAGAGATA CATGAGTGACTCGGGGATCG
GR CTTGTTGATGCGCAGACAGA CAAACTGCTCCTTGGTTGCT
DHAR GAGGTGAACCCAGAAGGGAA CCCACAGAGGCAAATTCAGG

Fig. 2

Effects of 6% FR supplementation on the phenotype of pepper seedlings"

Table 2

Effects of supplemental FR on the growth and biomass of pepper seedlings"

处理
Treatment		 株高
Plant height
(cm)		 茎粗
Stem thickness
(mm)		 第一节
间距
First internode length (cm)		 下胚轴
长度
Hypocotyl length
(cm)		 叶片数
Leaf number		 地上部鲜重
Aboveground
FW
(g)		 地下部鲜重
Underground
FW
(g)		 总鲜重
Total
FW
(g)		 地上部干重
Aboveground
DW
(g)		 地下部干重
Underground
DW
(g)		 总干重
Total
DW
(g)		 壮苗
指数
Seedling
index
自然光NL 9.88d 2.13bc 3.45c 3.10c 5.83a 1.72b 0.52b 2.21b 0.186bc 0.053bc 0.246b 0.118b
6% FR 11.37c 2.53a 3.33c 3.13c 6.00a 2.25a 0.65a 2.79a 0.223a 0.076a 0.297a 0.167a
13% FR 13.85b 2.28b 3.90b 3.40b 5.67a 1.72b 0.43c 2.19b 0.192b 0.061b 0.238b 0.110bc
20% FR 15.31a 1.96c 4.31a 3.90a 5.50a 1.65b 0.41c 0.173c 0.361b 0.047c 0.224b 0.096c

Fig. 3

Effects of 6% FR supplementation on the expression of CBF1 and antioxidant enzyme-related genes in pepper seedlings under low temperature stress"

Fig. 4

Effects of 6% FR supplementation on antioxidant enzyme activity and ABA content in pepper seedlings under low temperature stress"

Fig. 5

Effects of 6% FR supplementation on plant phenotype, Fv/Fm and REL in pepper seedlings under low temperature stress"

Fig. 6

Effects of 6% FR supplementation on antioxidant enzyme activity and ABA content in pepper seedlings under drought stress"

Fig. 7

Effects of 6% FR supplementation on plant phenotype, ΦPSII and REL in pepper seedlings under drought stress"

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