桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性

doi:10.3864/j.issn.0578-1752.2022.23.011

Abstract

Abstract:

【Objective】 The aim of this study was to identify members of the XTH gene family from peach, and to analyze the expression of PpXTHs in peach fruit with different textures during storage, which not only provided data for the research on the candidate PpXTHs involved in peach fruit softening, but also laid the foundation for further study on the PpXTHs function in peach fruit softening.【Method】 The HMM profiles of the Glyco_hydro_16 domain and XET_C domain were used to search all XTH proteins with the Hmmer 3.1 software in the peach protein database. The molecular weight, theoretical isoelectric point and other physicochemical properties were then predicted by the online tool ProtParam. PpXTHs subcellular localization were predicted by the online software Plant-mPLoc. The MEGA 11 software was used to construct a phylogenetic tree. The online tool MEME was used to analyze conserved motifs, the conserved motifs, conserved protein domains and gene structure maps were draw by Tbtools. According to the PpXTH gene family location information, chromosome mapping was carried out with MapChart software. The expressions of PpXTHs in peach fruit with different textures during storage were monitored by qRT-PCR..【Result】 A total of 27 PpXTH genes were systematically identified from peach, which were distributed on seven chromosomes. Based on the phylogenetic tree, the ancestral group, Ⅰ/Ⅱ subfamily, ⅢA subfamily and ⅢB subfamily were classified. In addition, according to the analysis of protein domains, all PpXTH gene family proteins had Glyco_hydro_16 and XET_C conserved domain. The results from qRT-PCR analysis showed that PpXTH33 belonging to the ⅢB subfamily was upregulated as the storage period increased in melting peach fruit, with the expression being markedly higher than that during the storage period of stony hard peach fruit. The positive clone sequencing was consistent with the coding sequence of the Prunus persica reference genome with a length of 924 bp for a 307 amino acid sequence. The PpXTH33 combined with green fluorescent protein may mainly located in the cell wall and nucleus detected by confocal laser scanning microscopy..【Conclusion】 All 27 PpXTHs protein structures contained two highly XTH conserved domains and the genes were distributed on seven chromosomes. The expression characteristics of PpXTH33 in peach fruit with different flesh textures during storage suggested that PpXTH33 was closely associated with peach fruit softening during storage.

Key words: peach, fruit softening, XTH gene family, gene expression, subcellular localization

GUO ShaoLei, XU JianLan, WANG XiaoJun, SU ZiWen, ZHANG BinBin, MA RuiJuan, YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage[J].Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.

Figures/Tables 8

Table 1

Table 2

Characteristic analysis of XTH gene family members in peach"

命名方式 Symbol	基因号 Gene ID	氨基酸数量 Number of amino acid	分子量 Mw (Da)	理论等电点 pI	亚细胞定位 Subcellular localization
PpXTH1a	Prupe.8G200900.1	287	33055.89	6.19	细胞壁 Cell wall
PpXTH1b	Prupe.8G201000.1	289	33545.54	6.31	细胞壁 Cell wall
PpXTH5	Prupe.1G210900.1	332	38568.82	8.73	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH6	Prupe.6G213600.1	291	33271.63	7.60	细胞壁 Cell wall
PpXTH8	Prupe.4G025600.1	300	34771.06	4.92	细胞壁 Cell wall
PpXTH9	Prupe.3G223200.1	296	33606.76	5.73	细胞壁 Cell wall
PpXTH10	Prupe.8G152500.1	295	34300.00	9.35	细胞壁 Cell wall
PpXTH16a	Prupe.4G072800.1	282	32460.74	9.44	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16b	Prupe.1G169700.1	277	31640.95	9.28	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16c	Prupe.3G172100.1	269	30385.02	8.58	细胞壁 Cell wall
PpXTH16d	Prupe.3G171500.1	285	32329.43	8.36	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16e	Prupe.3G171600.1	285	32263.44	8.91	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16f	Prupe.3G171700.1	282	32083.21	8.98	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16g	Prupe.3G172000.1	285	32135.26	8.70	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH16h	Prupe.3G171800.1	288	32353.36	8.48	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH25a	Prupe.1G088500.1	297	33791.75	4.80	细胞壁 Cell wall
PpXTH25b	Prupe.1G069800.1	285	31908.85	8.25	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH25c	Prupe.1G088800.1	284	32099.38	8.49	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH25d	Prupe.1G088600.1	293	32945.77	5.12	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH25e	Prupe.1G089000.1	299	33370.47	5.49	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH25f	Prupe.1G088900.1	296	32990.73	6.74	细胞壁 Cell wall, 细胞质 Cytoplasm
PpXTH26	Prupe.7G225000.1	290	32996.23	8.20	细胞壁 Cell wall
PpXTH28	Prupe.1G337000.1	338	38610.43	6.94	细胞壁 Cell wall
PpXTH30	Prupe.5G027800.1	359	41218.83	8.96	细胞壁 Cell wall
PpXTH32a	Prupe.7G128000.1	292	33720.45	9.52	细胞壁 Cell wall
PpXTH32b	Prupe.6G110300.1	351	39654.76	5.97	细胞壁 Cell wall
PpXTH33	Prupe.1G255100.1	307	34235.96	8.39	细胞壁 Cell wall

Table 2

Fig. 1

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References 52

[1]	YOSHIOKA H, HAYAMA H, TATSUKI M, NAKAMURA Y. Cell wall modification during development of mealy texture in the stony-hard peach “Odoroki” treated with propylene. Postharvest Biology and Technology, 2010, 55(1): 1-7. doi: 10.1016/j.postharvbio.2009.08.005
[2]	BRUMMELL D A, HARPSTER M H. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Molecular Biology, 2001, 47(1/2): 311-340. doi: 10.1023/A:1010656104304
[3]	MA M M, YUAN Y B, CHENG C X, ZHANG Y, YANG S L. The MdXTHB gene is involved in fruit softening in ‘Golden Del. Reinders’ (Malus pumila). Journal of the Science of Food and Agriculture, 2021, 101(2): 564-572. doi: 10.1002/jsfa.10668
[4]	WITASARI L D, HUANG F C, HOFFMANN T, ROZHON W, FRY S C, SCHWAB W. Higher expression of the strawberry xyloglucan endotransglucosylase/hydrolase genes FvXTH9 and FvXTH6 accelerates fruit ripening. The Plant Journal, 2019, 100(6): 1237-1253. doi: 10.1111/tpj.14512. doi: 10.1111/tpj.14512
[5]	HAN Y, BAN Q Y, LI H, HOU Y L, JIN M J, HAN S K, RAO J P. DkXTH8, a novel xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening. Scientific Reports, 2016, 6: 39155. doi: 10.1038/srep39155. doi: 10.1038/srep39155 pmid: 27966647
[6]	韩叶. 柿果实木葡聚糖内糖基转移/水解酶基因表达特性及功能分析[D]. 杨凌: 西北农林科技大学, 2017.
	HAN Y. Expression and functional analysis of xyloglucan endotransglycosylase/hydrolase genes in persimmon fruit[D]. Yangling: Northwest A＆F University, 2017. (in Chinese)
[7]	ZHU Q G, ZHANG Z K, RAO J P, HUBER D J, LV J Y, HOU Y L, SONG K H. Identification of xyloglucan endotransglucosylase/ hydrolase genes (XTHs) and their expression in persimmon fruit as influenced by 1-methylcyclopropene and gibberellic acid during storage at ambient temperature. Food Chemistry, 2013, 138(1): 471-477. doi: 10.1016/j.foodchem.2012.09.141. doi: 10.1016/j.foodchem.2012.09.141
[8]	SCHRÖDER R, ATKINSON R G, LANGENKÄMPER G, REDGWELL R J. Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit. Planta, 1998, 204(2): 242-251. doi: 10.1007/s004250050253. doi: 10.1007/s004250050253 pmid: 9487728
[9]	EKLÖF J M, BRUMER H. The XTH gene family: an update on enzyme structure, function, and phylogeny in xyloglucan remodeling. Plant Physiology, 2010, 153(2): 456-466. doi: 10.1104/pp.110.156844. doi: 10.1104/pp.110.156844 pmid: 20421457
[10]	BAUMANN M J, EKLÖF J M, MICHEL G, KALLAS A M, TEERI T T, CZJZEK M, BRUMER H. Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism. The Plant Cell, 2007, 19(6): 1947-1963. doi: 10.1105/tpc.107.051391. doi: 10.1105/tpc.107.051391
[11]	ROSE J K C, BRAAM J, FRY S C, NISHITANI K. The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: Current perspectives and a new unifying nomenclature. Plant and Cell Physiology, 2002, 43(12): 1421-1435. doi: 10.1093/pcp/pcf171. doi: 10.1093/pcp/pcf171 pmid: 12514239
[12]	COSGROVE D J. Growth of the plant cell wall. Nature Reviews Molecular Cell Biology, 2005, 6(11): 850-861. pmid: 16261190
[13]	ATKINSON R G, JOHNSTON S L, YAUK Y K, SHARMA, SCHRODER R. Analysis of xyloglucan endotransglucosylase/ hydrolase (XTH) gene families in kiwifruit and apple. Postharvest Biology and Technology, 2009, 51(2): 149-157. doi: 10.1016/j.postharvbio.2008.06.014
[14]	WU D, LIU A Q, QU X Y, LIANG J Y, SONG M. Genome-wide identification, and phylogenetic and expression profiling analyses of XTH gene families in Brassica rapa L. and Brassica oleracea L.. BMC Genomics, 2020, 21(1): 782. doi: 10.1186/s12864-020-07153-1
[15]	LI Q Y, LI H Y, YIN C Y, WANG X T, JIANG Q, ZHANG R, GE F F, CHEN Y D, YANG, L. Genome-wide identification and characterization of xyloglucan endotransglycosylase/hydrolase in Ananas comosus during development. Genes, 2019, 10(7): E537. doi: 10.3390/genes10070537. doi: 10.3390/genes10070537
[16]	FU M M, LIU C, WU F B. Genome-wide identification, characterization and expression analysis of xyloglucan endotransglucosylase/hydrolase genes family in barley (Hordeum vulgare). Molecules (Basel, Switzerland), 2019, 24(10): E 1935. doi: 10.3390/molecules24101935. doi: 10.3390/molecules24101935
[17]	WANG M, XU Z C, DING A M, Kong Y Z. Genome-wide identification and expression profiling analysis of the xyloglucan endotransglucosylase/hydrolase gene family in tobacco (Nicotiana tabacum L.). Genes, 2018, 9(6): 273. doi: 10.3390/genes9060273
[18]	ZHANG Z Y, WANG N, JIANG S H, XU H F, WANG Y C, WANG C Z, LI M, LIU J X, QU C Z, LIU W, WU S J, CHEN X L, CHEN X S. Analysis of the xyloglucan endotransglucosylase/hydrolase gene family during apple fruit ripening and softening. Journal of Agricultural and Food Chemistry, 2017, 65(2): 429-434. doi: 10.1021/acs.jafc.6b04536. doi: 10.1021/acs.jafc.6b04536 pmid: 28025888
[19]	HAN Y, ZHU Q G, ZHANG Z K, MENG K, HOU Y L, BAN Q Y, SUO J T, RAO J P. Analysis of xyloglucan endotransglycosylase/ hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening. PLoS ONE, 2015, 10(4): e0123668. doi: 10.1371/journal.pone.0123668
[20]	丛郁, 刘洪, 李慧, 颜志梅, 俞明亮, 常有宏.成熟砂梨果实木葡聚糖转移酶基因PpXTH1的克隆及其在夏季货架期的表达规律, 江苏农业学报, 2010, 26(1): 143-151.
	CONG Y, LIU H, LI H, YAN Z M, YU M L, CHANG Y H. Cloning of an xyloglucan endotransglycosylase/hydrolase gene (PpXTH1) from mature sandy pear fruit and its expression characteristics during summer shelf life. Jiangsu Journal of Agricultural Sciences, 2010, 26(1): 143-151. (in Chinese)
[21]	OPAZO M C, LIZANA R, STAPPUNG Y, DAVIS T M, HERRERA R, MOYA-LEÓN M A. XTHs from Fragaria vesca: genomic structure and transcriptomic analysis in ripening fruit and other tissues. BMC Genomics, 2017, 18(1): 852. doi: 10.1186/s12864-017-4255-8. doi: 10.1186/s12864-017-4255-8
[22]	LU W J, NAKANO R, KUBO Y, INABA A, JIANG Y M. Cloning and expression analysis of an XET cDNA in the peel and pulp of banana fruit ripening and softening. Acta Botanica Sinica, 2004, 46(3): 355-362.
[23]	YOKOYAMA R, NISHITANI K. A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant and Cell Physiology, 2001, 42(10): 1025-1033. doi: 10.1093/pcp/pce154. doi: 10.1093/pcp/pce154
[24]	YOKOYAMA R, ROSE J K C, NISHITANI K. A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis. Plant Physiology, 2004, 134(3): 1088-1099. pmid: 14988479
[25]	SALADIÉ M, ROSE J K, COSGROVE D J, CATALÁ C. Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action. The Plant Journal, 2006, 47(2): 282-295. doi: 10.1111/j.1365-313x.2006.02784.x. doi: 10.1111/j.1365-313x.2006.02784.x
[26]	HAN Y, BAN Q Y, HOU Y L, MENG K, SUO J T, RAO J P. Isolation and characterization of two persimmon xyloglucan endotransglycosylase/ hydrolase (XTH) genes that have divergent functions in cell wall modification and fruit postharvest softening. Frontiers in Plant Science, 2016, 7: 624.
[27]	CAMPBELL P, BRAAM J. In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis. Plant Journal, 1999, 18(4): 371-382. doi: 10.1046/j.1365-313x.1999.00459.x. doi: 10.1046/j.1365-313x.1999.00459.x
[28]	STROHMEIER M, HRMOVA M, FISCHER M, HARVEY A J, FINCHER G B, PLEISS J. Molecular modeling of family GH16 glycoside hydrolases: Potential roles for xyloglucan transglucosylases/ hydrolases in cell wall modification in the Poaceae. Protein Science, 2004, 13(12): 3200-3213. doi: 10.1110/ps.04828404. doi: 10.1110/ps.04828404
[29]	BEHAR H, GRAHAM S W, BRUMER H. Comprehensive cross- genome survey and phylogeny of glycoside hydrolase family 16 members reveals the evolutionary origin of EG16 and XTH proteins in plant lineages. Plant Journal, 2018, 95(6): 1114-1128. doi: 10.1111/tpj.14004. doi: 10.1111/tpj.14004
[30]	MICHAILIDIS G, ARGIRIOU A, DARZENTAS N, TSAFTARIS A. Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation. Journal of Plant Physiology, 2009, 166(4): 403-416. doi: 10.1016/j.jplph.2008.06.013. doi: 10.1016/j.jplph.2008.06.013 pmid: 18789555
[31]	MUÑOZ-BERTOMEU J, MIEDES E, LORENCES E P. Expression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruits. Journal of Plant Physiology, 2013, 170(13): 1194-1201. doi: 10.1016/j.jplph.2013.03.015
[32]	FINN R D, COGGILL P, EBERHARDT R Y, EDDY S R, MISTRY J, MITCHELL A L, POTTER S C, PUNTA M, QURESHI M, SANGRADOR-VEGAS A, SALAZAR G A, TATE J, BATEMAN A. The Pfam protein families database: Towards a more sustainable future. Nucleic Acids Research, 2015, 44(D1): D279-D285. doi: 10.1093/nar/gkv1344. doi: 10.1093/nar/gkv1344
[33]	GOODSTEIN D M, SHU S Q, HOWSON R, NEUPANE R, HAYES R D, FAZO J, MITROS T, DIRKS W, HELLSTEN U, PUTNAM N, ROKHSAR D S. Phytozome: A comparative platform for green plant genomics. Nucleic Acids Resesearch, 2012, 40(D1): D1178-D1186. doi: 10.1093/nar/gkr944. doi: 10.1093/nar/gkr944
[34]	MARCHLER-BAUER A, BO Y, HAN L Y, HE J E, LANCZYCKI C J, LU S N, CHITSAZ F, DERBYSHIRE M K, GEER R C, GONZALES N R, GWADZ M, HURWITZ D I, LU F, MARCHLER G H, SONG J S, THANKI N, WANG Z X, YAMASHITA R A, ZHANG D C, ZHENG C J, GEER L Y, BRYANT S H. CDD/SPARCLE: Functional classification of proteins via subfamily domain architectures. Nucleic Acids Research, 2016, 45(D1): D200-D203. doi: 10.1093/nar/gkw1129. doi: 10.1093/nar/gkw1129
[35]	WILKINS M R, GASTEIGER E, BAIROCH A, SANCHEZ J C, WILLIAMS K L, APPEL R D, HOCHSTRASSER D F. Protein identification and analysis tools in the ExPASy server. Methods in Molecular Biology, 1999, 112: 531-552. doi: 10.1385/1-59259-584-7:531. doi: 10.1385/1-59259-584-7:531 pmid: 10027275
[36]	CHOU K C, SHEN H B. Cell-PLoc: A package of web servers for predicting subcellular localization of proteins in various organisms. Nature Protocols, 2008, 3(2): 153-162. doi: 10.1038/nprot.2007.494. doi: 10.1038/nprot.2007.494
[37]	BAILEY T L, JOHNSON J, GRANT C E, NOBLE W S. The MEME Suite. Nucleic Acids Research, 2015, 43(W1): W39-W49. doi: 10.1093/nar/gkv416
[38]	CHEN C J, CHEN H, ZHANG Y, THOMAS H R, FRANK M H, HE Y H, XIA R. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 2020, 13(8): 1194-1202. doi: 10.1016/j.molp.2020.06.009. doi: S1674-2052(20)30187-8 pmid: 32585190
[39]	LAMESCH P, BERARDINI T Z, LI D H, SWARBRECK D, WILKS C, SASIDHARAN R, MULLER R, DREHER K, ALEXANDER D L, GARCIA-HERNANDEZ M, KARTHIKEYAN A S, LEE C H, NELSON W D, PLOETZ L, SINGH S, WENSEL A, HUALA E. The Arabidopsis Information Resource (TAIR): Improved gene annotation and new tools. Nucleic Acids Research, 2011, 40(D1): D1202-D1210. doi: 10.1093/nar/gkr1090. doi: 10.1093/nar/gkr1090
[40]	JOHANSSON P, BRUMER H, BAUMANN M J, KALLAS A M, HENRIKSSON H, DENMAN S E, TEERI T T, JONES T A. Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding. The Plant Cell, 2004, 16(4): 874-886. doi:10.1105/tpc.020065. doi: 10.1105/tpc.020065
[41]	TAMURA K, STECHER G, KUMAR S. MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 2021, 38(7): 3022-3027. doi: 10.1093/molbev/msab120. doi: 10.1093/molbev/msab120 pmid: 33892491
[42]	ROBERT X, GOUET P. Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Research, 2014, 42(W1): W320-W324. doi: 10.1093/nar/gku316. doi: 10.1093/nar/gku316
[43]	VOORRIPS R E. MapChart: software for the graphical presentation of linkage maps and QTLs. Journal of Heredity, 2002, 93(1): 77-78. doi: 10.1093/jhered/93.1.77. doi: 10.1093/jhered/93.1.77 pmid: 12011185
[44]	TONG Z G, GAO Z H, WANG F, ZHOU J, ZHANG Z. Selection of reliable reference genes for gene expression studies in peach using real-time PCR. BMC Molecular Biology, 2009, 10: 71. doi: 10.1186/1471-2199-10-71. doi: 10.1186/1471-2199-10-71 pmid: 19619301
[45]	LIVAK K J, SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
[46]	王昊, 尹莲, 刘洁霞, 贾丽丽, 丁旭, 沈迪, 冯凯, 徐志胜, 熊爱生. 类胡萝卜素裂解双加氧酶基因AgCCD4调控芹菜不同组织的着色. 中国农业科学, 2021, 54(15): 3279-3294.
	WANG H, YIN L, LIU J X, JIA L L, DING X, SHEN D, FENG K, XU Z S, XIONG A S. The carotenoid cleavage dioxygenases gene AgCCD4 regulates the pigmentation of celery tissues with different colors. Scientia Agricultura Sinica, 2021, 54(15): 3279-3294. (in Chinese)
[47]	徐小迪, 李博强, 秦国政, 陈彤, 张占全, 田世平. 果实采后品质维持的分子基础与调控技术研究进展. 园艺学报, 2020, 47(8): 1595-1609.
	XU X D, LI B Q, QIN G Z, CHEN T, ZHANG Z Q, TIAN S P. Molecular basis and regulation strategies for quality maintenance of postharvest fruit. Acta Horticulturae Sinica, 2020, 47(8): 1595-1609. (in Chinese)
[48]	IQBAL N, KHAN N A, FERRANTE A, TRIVELLINI A, FRANCINI A, KHAN M I R. Ethylene role in plant growth, development and senescence: interaction with other phytohormones. Frontiers in Plant Science, 2017, 8: 475. doi: 10.3389/fpls.2017.00475. doi: 10.3389/fpls.2017.00475 pmid: 28421102
[49]	ZHAI Z F, FENG C, WANG Y Y, SUN Y T, PENG X, XIAO Y Q, ZHANG X, ZHOU X, JIAO J L, WANG W L. Genome-wide identification of the xyloglucan endotransglucosylase/hydrolase (XTH) and polygalacturonase (PG) genes and characterization of their role in fruit softening of sweet cherry. International Journal of Molecular Sciences, 2021, 22(22): 12331. doi: 10.3390/ijms222212331. doi: 10.3390/ijms222212331
[50]	CHENG Z H, ZHANG X M, YAO W J, GAO Y, ZHAO K, GUO Q, ZHOU B R, JIANG T B. Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar. BMC Genomics, 2021, 22(1): 804. doi: 10.1186/s12864-021-08134-8. doi: 10.1186/s12864-021-08134-8 pmid: 34749656
[51]	杨勇, 马瑞娟, 张斌斌, 宋志忠, 张春华, 郭绍雷, 俞明亮. 不同溶质桃果实的软化与乙烯合成相关基因的差异表达. 园艺学报, 2015, 42(10): 1869-1878.
	YANG Y, MA R J, ZHANG B B, SONG Z Z, ZHANG C H, GUO S L, YU M L. Differential expression analysis in fruit softening and ethylene biosynthetic pathways in peaches of different flesh textures. Acta Horticulturae Sinica, 2015, 42(10): 1869-1878. (in Chinese)
[52]	CHEEVARUNGNAPAKUL K, KHAKSAR G, PANPETCH P, BOONJING P, SIRIKANTARAMAS S. Identification and functional characterization of genes involved in the biosynthesis of caffeoylquinic acids in sunflower (Helianthus annuus L.). Frontiers in Plant Science, 2019, 10: 968. doi: 10.3389/fpls.2019.00968. doi: 10.3389/fpls.2019.00968

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基因 Gene	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
PpXTH1a	ATGGCTTTCTCAGTGGTTCGT	AAAGCCGGACTTCTCTTCCA
PpXTH1b	GCAATCACGCTGCAGACAAA	TAGGCTGTGTTGGGTAGGGA
PpXTH5	CCGCAGCTTACCACTCCTAC	AGAATTTGGCCTCCACCGAG
PpXTH6	GAGTTCTTGGGGAACCGGAC	GGGTTGGAGCTTGGGGTATG
PpXTH8	GATGGGTGCCAATGGGAAGA	GGCAAAGTTGGGTAGCGTTC
PpXTH9	CAAGGACCAAGCTATGGGGG	CTGCTGCACTTCTTTGCGTT
PpXTH10	GGAAATGTGGCAGGCAAACC	AGCTGTCGCCATTCCATAGG
PpXTH16a	GCCAAACCGCGATGAGATTG	ACGCTGAGGGTTCCAAAGAA
PpXTH16b	AAACGGACTGGTCAAAGGCA	CACTCAACTGGAGGGCCTTG
PpXTH16c	AAGACTGACTGGAGCCATGC	CGGGAACTTAGGACGGTCAG
PpXTH16d	CTGGAACTCTCAGCGCATC	AGGAGCTTGTGTCCAGTCAG
PpXTH16e	TCTTCTCAAGGGCCAACCCA	GGCTGTTCCAGACAATGGAGT
PpXTH16f	ATGACTTCTTCCAAGGTCACTGT	TGAGAAGCTGTCCTCCGTTG
PpXTH16g	CCCTACACTCTCCACACAAAC	GGGTTGGCTTTTGGGAAATGG
PpXTH16h	GGTGCTCATTCTTTCTTTGGTC	TCTGCATGTCAATCCTGCCC
PpXTH25a	CCACAAGAGGTGGACTGGTG	ACCCATGCCATCTTCCCTTG
PpXTH25b	CAGGCTCTTCCCGTTGTTCT	GTGGGAAGCGCTTGGTATCT
PpXTH25c	AACGCCAATGCTTGCGTATG	TTCAGGAGGGAGGCCTTGT
PpXTH25d	ACTGGGCTACAAGAGGAGGT	TCTTGGCCGGTGGAGTCTAA
PpXTH25e	AATGCCTGCGTATGGTCCTC	AGCCTTGGGGAAATCTCTTGG
PpXTH25f	AGAGCTCCTTTCACTGCTTCC	CAGCCTCTCCTGCCTTGTTA
PpXTH26	CAGGGGATTGCTTACCCCAA	TGTCTCCGTTCCACTTGCAG
PpXTH28	CTACACGGCTGTGCAGTTGA	TGTGACGGGGTCAAACACTC
PpXTH30	CAGGTTCAGGCAGCGATACA	GATTGCGATTGCGGGTTCTC
PpXTH32a	CTGGGGTCCTCAGCATCAAA	GTCATGGTACCCCGGATGAG
PpXTH32b	TGTGGGGTCCACAGATCAC	CCTGATCAAACCCTACTGTGCT
PpXTH33	CTACACAAGCCCAAGCGTTC	ACAACCCCAGAAGTGAGACC
TEF2	GGTGTGACGATGAAGAGTGATG	TGAAGGAGAGGGAAGGTGAAAG
pCAMBIA1302-PpXTH33-GFP	AACACGGGGGACTCTTGAccatgg ATGGCATTTTTGCAGGGAAAAC TCC	GAAAAGTTCTTCTCCTTTactagt TTTGCACTCTGGTGGCATGAC

Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage

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