螺虫乙酯抑制西花蓟马卵孵化的蛋白质组学分析

doi:10.3864/j.issn.0578-1752.2022.15.006

Abstract

Abstract:

【Objective】Previous studies showed that the 0 h old eggs of Frankliniella occidentalis after 24 h treated with spirotetramat resulted in the cracking of eggs shell and inhibited the hatching of eggs. The objective of this study is to investigate the causes of morphological and structural changes of 0 h old eggs of F. occidentalis after 24 h treated with spirotetramat, and to find the key proteins and reveal the molecular mechanism of spirotetramat inhibiting the hatching of 0 h old eggs of F. occidentalis. 【Method】The 0 h old eggs of F. occidentalis were treated with 14.42 mg·L^-1 spirotetramat for 24 h, and the Label-free quantitative proteomics technique was used to analyze differentially expressed proteins between the spirotetramat-treated group and the control group. Gene Ontology (GO) enrichment analysis and KEGG pathway analysis were used for functional annotation and pathway analysis of the differentially expressed proteins, and the expression of differential expressed proteins was verified by parallel reaction monitoring (PRM).【Result】A total of 204 proteins were differentially expressed after treatment with spirotetramat, of which 124 proteins were up-regulated and 80 proteins were down-regulated. Bioinformatics analysis showed that the up-regulated differentially expressed proteins were mainly involved in substance synthesis and metabolic pathways, while the down-regulated differentially expressed proteins were mainly participated in the defense response pathway to external stimuli. In addition, the results of PRM were consistent with the proteomics results. The results showed that the protein expression levels of GTP cyclohydrolase 1-1, GTP cyclohydrolase 1-2, glutathione S-transferase-like and fatty acyl-CoA reductase were significantly up-regulated, while the expression levels of endocuticle structural glycoprotein SgAbd-2-like, superoxide dismutase [Cu-Zn]-like and O-acetyl transferase in 0 h old eggs of F. occidentalis after 24 h treated with spirotetramat were significantly down-regulated, especially endocuticle structure glycoprotein SgAbd-2-like.【Conclusion】The above proteins may play multiple functions in the hatching process of F. occidentalis eggs, the endocuticle structure glycoprotein SgAbd-2-like is related to insect epidermal differentiation and other life activities, it was speculated that the expression of this protein was inhibited by spirotetramat, which resulted in egg shell rupture of F. occidentalis 0 h old eggs, the endocuticle structure glycoprotein SgAbd-2-like may play an important role in the hatching process of F. occidentalis 0 h old eggs.

Key words: Frankliniella occidentalis, spirotetramat, egg, proteomics, hatching

ZHOU GuiYing,YANG XiaoMin,TENG ZiWen,SUN LiJuan,ZHENG ChangYing. Quantitative Proteomic Analysis of Spirotetramat Inhibiting Hatching of Frankliniella occidentalis Eggs[J].Scientia Agricultura Sinica, 2022, 55(15): 2938-2948.

Figures/Tables 5

Fig. 1

Table 1

Differentially expressed protein results statistics"

蛋白名称 Protein name	蛋白登录号 Protein ID	肽段数 Peptide count	特殊肽段数 Unique peptide count	分子量 Molecular weight (kD)	变化倍数Fold change	P值 P value
三磷酸鸟苷环水解酶1-1 GTP cyclohydrolase 1-1	A0A6J1TMB5	3	3	18.50	13.65	0.00009
三磷酸鸟苷环水解酶1-2 GTP cyclohydrolase 1-2	A0A6J1T9W9	3	3	17.58	11.16	0.00062
类谷胱甘肽硫-转移酶Glutathione S-transferase-like	A0A6J1SUA2	13	13	23.29	7.37	0.00005
脂酰辅酶A还原酶Fatty acyl-CoA reductase	A0A6J1TJA8	4	4	57.99	4.04	0.00067
超长链脂肪酸蛋白质的延伸 Elongation of very long chain fatty acids protein	A0A6J1SJR5; A0A6J1SRS0	2	2	39.09	2.75	0.03058
甘露聚糖酶Mannanase	A0A6J1T5W2	8	8	108.14	2.59	0.03446
谷胱甘肽过氧化物酶Glutathione peroxidase	A0A6J1TAR5; A0A6J1T4F1	11	11	18.60	2.13	0.00450
类胰脂肪酶相关蛋白3 Pancreatic lipase-related protein 3-like	A0A6J1STX0	3	3	42.11	1.99	0.01464
酪氨酸蛋白激酶Tyrosine-protein kinase	A0A6J1RYS7	3	3	95.47	1.99	0.00560
Ras相关蛋白Rab-32亚型X9 Ras-related protein Rab-32 isoform X9	A0A6J1SY76; A0A6J1SSP5; A0A6J1STV6; A0A6J1STV8; A0A6J1T0S5; A0A6J1SY71; A0A6J1SSN8; A0A6J1STV1; A0A6J1STV2	3	3	24.05	1.96	0.00719
泛素结合酶E2 G1 Ubiquitin-conjugating enzyme E2 G1	A0A6J1TK58	3	3	19.44	1.89	0.04406
脂肪酸结合蛋白,肌肉亚型X2 Fatty acid-binding protein, muscle isoform X2	A0A6J1RYX2; A0A6J1RRR7	13	13	15.01	1.84	0.02624
类谷胱甘肽S-转移酶 θ-1 Glutathione S-transferase theta-1-like	A0A6J1RZW4	5	5	25.72	1.83	0.04168
脂酰辅酶A还原酶 Fatty acyl-CoA reductase	A0A6J1SPV4; A0A6J1SJC4	6	6	57.97	1.77	0.03626
类85/88 kD钙非依赖性磷脂酶A2 85/88 kD calcium-independent phospholipase A2-like	A0A6J1RWA1	3	3	87.87	1.65	0.03396
ATP依赖型RNA解旋酶A亚型X1 ATP-dependent RNA helicase A isoform X1	A0A6J1S206; A0A6J1S943	7	7	140.50	1.64	0.01121
Ras相关蛋白rab7 Ras-related protein rab7	A0A6J1STG7	8	8	23.30	1.60	0.01576
核糖体生物合成蛋白BMS1同源异构体 X2 Ribosome biogenesis protein BMS1 homolog isoform X2	A0A6J1S7D5; A0A6J1S5Z1	6	6	151.97	1.57	0.01277
阿法丁异构体X6 Afadin isoform X6	A0A6J1SEG9; A0A6J1S933; A0A6J1S9F8; A0A6J1SGI5; A0A6J1SEG7; A0A6J1SAN7	10	10	221.95	1.57	0.00601
脂肪细胞质膜相关蛋白 Adipocyte plasma membrane-associated protein	A0A6J1SQ17	15	15	65.63	1.56	0.03344
ATP结合盒亚家族F成员2 ATP-binding cassette sub-family F member 2	A0A6J1SA46	13	13	71.60	0.64	0.00254
类蛋白阻塞物-E Protein obstructor-E-like	A0A6J1TAJ9	5	5	40.81	0.63	0.00146
O-乙酰基转移酶O-acetyl transferase	A0A6J1S6K4; A0A6J1S111	2	2	57.82	0.63	0.02456
RNA解旋酶RNA helicase	A0A6J1S314; A0A6J1S9L2	24	20	60.17	0.62	0.00058
类鸟嘌呤核苷酸结合蛋白1 Guanine nucleotide-binding protein-like 1	A0A6J1T023	9	9	68.61	0.59	0.03401
T复合蛋白1亚基epsilon T-complex protein 1 subunit epsilon isoform X1	A0A6J1S5J0; A0A6J1S3Y9	30	30	59.55	0.59	0.02217
δ-1-吡咯啉-5-羧酸合酶Delta-1-pyrroline-5-carboxylate synthase	A0A6J1THW9	8	8	83.84	0.59	0.03537
泛素结合酶E2 N Ubiquitin-conjugating enzyme E2 N	A0A6J1RWY7	5	5	17.19	0.58	0.01814
酪蛋白激酶I同种型X2 Casein kinase I isoform X2	A0A6J1SSW6; A0A6J1T0Y4	6	6	38.35	0.58	0.00652
微管蛋白α链Tubulin alpha chain	A0A6J1TCU9	16	1	49.99	0.55	0.00865
类Ras相关蛋白Rab-18-B Ras-related protein Rab-18-B-like	A0A6J1RZP1	2	2	23.35	0.52	0.04714
40S核糖体蛋白S24 40S ribosomal protein S24	A0A6J1TJB9; A0A6J1TJZ2	5	5	15.16	0.48	0.03436
上游激活因子亚基spp27 Upstream activation factor subunit spp27	A0A6J1RYK9	10	10	28.57	0.48	0.04572
类蛋白多糖4亚型X1 Proteoglycan 4-like isoform X1	A0A6J1TNX9; A0A6J1TSU0	14	14	146.33	0.38	0.03717
丝裂原活化蛋白激酶 Mitogen-activated protein kinase	A0A6J1SZU8; A0A6J1TD29; A0A6J1S2K1	5	4	42.50	0.33	0.01435
谷氨酰胺-果糖-6-磷酸转氨酶（异构化） Glutamine-fructose-6-phosphate transaminase (isomerizing)	A0A6J1SYR9; A0A6J1T384	22	22	76.10	0.33	0.00019
RNA解旋酶RNA helicase	A0A6J1T1T7	11	11	69.78	0.33	0.02672
细胞色素P450 Cytochrome P450 6a2-like	A0A6J1SXB7; F1JYR2; F1JYR1; A0A6J1SYI1; A0A6J1T5I4; A0A6J1T3E3; A0A6J1SJ06	11	10	60.04	0.28	0.02177
类铜/锌-超氧化物歧化酶Superoxide dismutase [Cu-Zn]-like	A0A6J1T4V3	4	4	18.72	0.19	0.00577
类内表皮结构糖蛋白SgAbd-2 Endocuticle structural glycoprotein SgAbd-2-like	A0A6J1T9H5	6	1	11.86	0.06	0.00211

Table 1

Fig. 2

Fig. 3

Fig. 4

References 33

[1]	KIRK W D J, TERRY L I. The spread of the Western flower thrips Frankliniella occidentalis (Pergande). Agricultural and Forest Entomology, 2003, 5(4): 301-310. doi: 10.1046/j.1461-9563.2003.00192.x
[2]	张友军, 吴青君, 徐宝云, 朱国仁. 危险性外来入侵生物--西花蓟马在北京发生危害. 植物保护, 2003, 29(4): 58-59.
	ZHANG Y J, WU Q J, XU B Y, ZHU G R. Dangerous alien invasive species - Occurrence and damages of Frankliniella occidentalis in Beijing. Plant Protection, 2003, 29(4): 58-59. (in Chinese)
[3]	郑长英, 刘云虹, 张乃芹, 赵希丽. 山东省发现外来入侵有害生物-西花蓟马. 青岛农业大学学报(自然科学版), 2007, 24(3): 172-174.
	ZHENG C Y, LIU Y H, ZHANG N Q, ZHAO X L. Invaded insect pest - Frankliniella occidentalis first reported in Shandong Province. Journal of Qingdao Agricultural University (Natural Science), 2007, 24(3): 172-174. (in Chinese)
[4]	GERMAN T L, ULLMAN D E, MOYER J W. Tospoviruses: Diagnosis, molecular biology, phylogeny, and vector relationships. Annual Review of Phytopathology, 1992, 30: 315-348. doi: 10.1146/annurev.py.30.090192.001531
[5]	ULLMAN D E, SHERWOOD J L, GERMAN T L. Thrips as vectors of plant pathogens//Thrips as Crop Pests, 1997: 539-565.
[6]	YANG X M, ZHOU G Y, SUN L J, ZHENG C Y. Ovicidal activity of spirotetramat and its effect on hatching, development and formation of Frankliniella occidentalis egg. Scientific Reports, 2021, 11(1): 20751. doi: 10.1038/s41598-021-00160-6
[7]	RENOZ F, DEMETER S, DEGAND H, NICOLIS S C, LEBBE O, MARTIN H, DENEUBOURG J L, FAUCONNIER M L, MORSOMME P, HANCE T. The modes of action of Mentha arvensis essential oil on the granary weevil Sitophilus granarius revealed by a label-free quantitative proteomic analysis. Journal of Pest Science, 2022, 95: 381-395. doi: 10.1007/s10340-021-01381-4
[8]	KOCOUREK F, STARA J, SOPKO B, TALACKO P, HARANT K, HOVORKA T, ERBAN T. Proteogenomic insight into the basis of the insecticide tolerance/resistance of the pollen beetle Brassicogethes (Meligethes) aeneus. Journal of Proteomics, 2021, 233: 104086. doi: 10.1016/j.jprot.2020.104086
[9]	CHEN J, CUI D N, ULLAH H, HAO K, TU X B, ZHANG Z H. Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase. FEBS Open Bio, 2020, 10(5): 707-717. doi: 10.1002/2211-5463.12825
[10]	RAUNIYAR N. Parallel reaction monitoring: A targeted experiment performed using high resolution and high mass accuracy mass spectrometry. International Journal of Molecular Sciences, 2015, 16(12): 28566-28581. doi: 10.3390/ijms161226120
[11]	RONSEIN G E, PAMIR N, VON HALLER P D, KIM D S, ODA M N, JARVIK G P, HEINECKE J W. Parallel reaction monitoring (PRM) and selected reaction monitoring (SRM) exhibit comparable linearity, dynamic range and precision for targeted quantitative HDL proteomics. Journal of Proteomics, 2015, 113: 388-399. doi: 10.1016/j.jprot.2014.10.017
[12]	SI W Y, WANG Q J, LI Y, DONG D J. Label-free quantitative proteomic analysis of insect larval and metamorphic molts. BMC Developmental Biology, 2020, 20(1): 24. doi: 10.1186/s12861-020-00227-z
[13]	TANG B Z, MENG E, ZHANG H J, ZHANG X M, ASGARI S, LIN Y P, LIN Y Y, PENG Z Q, QIAO T, ZHANG X F, HOU Y M. Combination of label-free quantitative proteomics and transcriptomics reveals intraspecific venom variation between the two strains of Tetrastichus brontispae, a parasitoid of two invasive beetles. Journal of Proteomics, 2019, 192: 37-53. doi: 10.1016/j.jprot.2018.08.003
[14]	SILVA R S, ARCANJO L P, SOARES J R S, FERREIRA D O, SERRÃO J E, MART INS J C, COSTA Á H, PICANÇO M C. Insecticide toxicity to the borer Neoleucinodes elegantalis (Guenée) (Lepidoptera: Crambidae): Developmental and egg-laying effects. Neotropical Entomology, 2018, 47(2): 318-325. doi: 10.1007/s13744-017-0553-8
[15]	SANTOS N D, DE MOURA K S, NAPOLEAO T H, SANTOS G K, COELHO L C, NAVARRO D M, PAIVA P M. Oviposition-stimulant and ovicidal activities of Moringa oleifera Lectin on Aedes aegypti. PLoS ONE, 2012, 7(9): e44840. doi: 10.1371/journal.pone.0044840
[16]	郑长英, 马亚斌, 冯志国, 李洪刚, 李红霞, 李娜, 张彬. 一种大量收集西花蓟马卵的方法: CN105210997A[P].(2018-01-26) [2022-01-12].
	ZHENG C Y, MA Y B, FENG Z G, LI H G, LI H X, LI N, ZHANG B. A method for mass collection of Frankliniella occidentalis eggs: CN105210997A[P].(2018-01-26) [2022-01-12]. (in Chinese)
[17]	刘俊珍. 颅内动脉瘤破裂风险相关血清蛋白差异表达的分析[D]. 南昌: 南昌大学, 2021.
	LIU J Z. Analysis of the difference expression of serum protein related to the risk of intracranial aneurysm rupture[D]. Nanchang: Nanchang University, 2021. (in Chinese)
[18]	涂晓辉. 菜粉蝶超氧化物歧化酶基因的鉴定、表达模式与抗氧化功能分析[D]. 合肥: 安徽农业大学, 2020.
	TU X H. Identification, expression pattern, and antioxidant function of superoxide dismutase genes from Pieris rapae[D]. Hefei: Anhui Agricultural University, 2020. (in Chinese)
[19]	ZHUO X F, LING X Y, GUO H J, ZHU-SALZMAN K, SUN Y C. Serratia symbiotica enhances fatty acid metabolism of pea aphid to promote host development. International Journal of Molecular Sciences, 2021, 22(11): 5951. doi: 10.3390/ijms22115951
[20]	ZHANG S, LUO J Y, LV L M, WANG C Y, LI C H, ZHU X Z, CUI J J. Effects of Lysiphlebia japonica (Ashmead) on cotton-melon aphid Aphis gossypii Glover lipid synthesis. Insect Molecular Biology, 2015, 24(3): 348-357. doi: 10.1111/imb.12162
[21]	HEMINGWAY J, HAWKES N J, MCCARROLL L, RANSON H. The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology, 2004, 34(7): 653-665. doi: 10.1016/j.ibmb.2004.03.018
[22]	李茂业, 黄岩, 蒋秀云, 何佶弦, 刘苏. 小地老虎谷胱甘肽-S-转移酶AiGSTs1的分子特性及其对杀虫剂胁迫的响应. 应用昆虫学报, 2021, 58(4): 938-948.
	LI M Y, HUANG Y, JIANG X Y, HE J X, LIU S. Molecular characterization of glutathione-S-transferase (AiGSTs1) in Agrotis ipsilon subject to insecticide stress. Chinese Journal of Applied Entomology, 2021, 58(4): 938-948. (in Chinese)
[23]	杨晓敏. 螺虫乙酯抑制西花蓟马卵孵化的生理生化机制[D]. 青岛: 青岛农业大学, 2021.
	YANG X M. Physiological and biochemical mechanism of spirotetramat inhibiting hatching of Frankliniella occidentalis[D]. Qingdao: Qingdao Agricultural University, 2021. (in Chinese)
[24]	胡艳红. 白蜡虫脂酰辅酶A还原酶基因 (EpFAR) 的克隆表达与功能研究[D]. 南京: 南京林业大学, 2018.
	HU Y H. Cloning, expression characterization and functional assay of a fatty acyl-CoA reductase gene in scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidea)[D]. Nanjing: Nanjing Forestry University, 2018. (in Chinese)
[25]	李晓龙. 扶桑绵粉蚧脂质合成相关基因的特性和功能研究[D]. 杭州: 浙江农林大学, 2016.
	LI X L. Characterization and functions of lipid biosynthesis related genes of the cotton mealbug Phenacoccus solenopsis[D]. Hangzhou: Zhejiang A&F University, 2016. (in Chinese)
[26]	侯丽. 两种小GTP酶Rab4b,Rab32及蛋白激酶C delta (PKC δ) 在棉铃虫变态过程中的功能研究[D]. 济南: 山东大学, 2012.
	HOU L. Function analysis of Rab4b, Rab32 and protein kinase C delta (PKC δ) during metamorphosis in Helicoverpa armigera[D]. Ji’nan: Shandong University, 2012. (in Chinese)
[27]	WILLIS J H. Structural cuticular proteins from arthropods: Annotation, nomenclature, and sequence characteristics in the genomics era. Insect Biochemistry and Molecular Biology, 2010, 40(3): 189-204.
[28]	LU J B, LUO X M, ZHANG X Y, PAN P L, ZHANG C X. An ungrouped cuticular protein is essential for normal endocuticle formation in the brown planthopper. Insect Biochemistry and Molecular Biology, 2018, 100: 1-9. doi: 10.1016/j.ibmb.2018.06.001
[29]	XIONG G, TONG X L, GAI T T, LI C, QIAO L, MONTEIRO A, HU H, HAN M J, DING X, WU S Y, XIANG Z H, LU C, DAI F Y. Body shape and coloration of silkworm larvae are influenced by a novel cuticular protein. Genetics, 2017, 207(3): 1053-1066. doi: 10.1534/genetics.117.300300
[30]	QIAO L, XIONG G, WANG R X, HE S Z, CHEN J, TONG X L, HU H, LI C L, GAI T T, XIN Y Q, LIU X F, CHEN B, XIANG Z H, LU C, DAI F Y. Mutation of a cuticular protein, BmorCPR2, alters larval body shape and adaptability in silkworm, Bombyx mori. Genetics, 2014, 196(4): 1103-1115. doi: 10.1534/genetics.113.158766
[31]	SUMAN D S, WANG Y, BILGRAMI A L, GAUGLER R. Ovicidal activity of three insect growth regulators against Aedes and Culex mosquitoes. Acta Tropica, 2013, 128(1): 103-109. doi: 10.1016/j.actatropica.2013.06.025
[32]	贾盼, 张晶, 杨洋, 刘卫敏, 张建珍, 赵小明. 飞蝗内表皮结构糖蛋白基因 LmAbd-2 的表达与功能分析. 中国农业科学, 2019, 52(4): 651-660.
	JIA P, ZHANG J, YANG Y, LIU W M, ZHANG J Z, ZHAO X M. Expression and function analysis of endocuticle structural glycoprotein gene LmAbd-2 in Locusta migratoria. Scientia Agricultura Sinica, 2019, 52(4): 651-660. (in Chinese)
[33]	袁江江. 西花蓟马抗药性监测及SgAbd-1-like基因的克隆和定量分析[D]. 荆州: 长江大学, 2019.
	YUAN J J. Resistance monitoring and cloning and quantitative analysis of SgAbd-1-like gene in Frankliniella occidentalis (Pergande)[D]. Jingzhou: Yangtze University, 2019. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Quantitative Proteomic Analysis of Spirotetramat Inhibiting Hatching of Frankliniella occidentalis Eggs

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 5

References 33

Related Articles 15

Metrics

Comments

Recommended 0

[1]	PEI HongXia, WANG LuYao, JIANG YaPing, LI ShengMei, GAO JingXia. Identification of Nuclear Male Sterility Genes in Pepper Based on BSA-Seq and Proteomics [J]. Scientia Agricultura Sinica, 2026, 59(3): 637-654.
[2]	ZHANG YiRu, HAN Xue, YAO XinJie, FENG Jun, WEI AiLi, LI WenChao, ZHANG Bin, HAN YuanHuai, LI HongYing. Integrated Multi-Omics Elucidates the Pigmentation Dynamics During Post-Harvest Maturation in Foxtail Millet (Setaria italica) [J]. Scientia Agricultura Sinica, 2025, 58(9): 1702-1718.
[3]	ZHANG LinLin, GONG Rui, CUI YanLing, ZHONG XiongHui, LI Ye, LI RanHong, QIAN ZongWei. Effect Analysis of SmWRKY30 in Eggplant Resistance to Ralstonia solanacearum by Virus Induced Gene Silencing (VIGS) [J]. Scientia Agricultura Sinica, 2025, 58(3): 548-563.
[4]	YANG YongNian, ZENG XiangCui, LIU QingSong, LI RuYue, LONG RuiCai, CHEN Lin, WANG Xue, HE Fei, KANG JunMei, LI MingNa. Differential Proteomic Analysis of Alfalfa Seedlings Under Salt- Alkaline Stress [J]. Scientia Agricultura Sinica, 2025, 58(21): 4512-4527.
[5]	LIU HongXiang, ZHANG XuePing, WANG YiFei, WANG ZhiCheng, GU HaoTian, SONG WeiTao, TAO ZhiYun, XU WenJuan, ZHANG ShuangJie, LU LiZhi, LI HuiFang, ZHU ChunHong. Genome-Wide Association Study of Egg Production Traits in Jinding Duck [J]. Scientia Agricultura Sinica, 2025, 58(15): 3145-3158.
[6]	ZHANG HuiHui, KANG HanYe, LIU Hui, ZHANG JinRui, HUO Fan, GUO WeiQi, YE XiaoFang, JI Rong, HU HongXia. Differentially Expressed Proteins Analysis of Locusta migratoria Infected by Paranosema locustae Based on TMT Proteomics Technique [J]. Scientia Agricultura Sinica, 2024, 57(24): 4884-4893.
[7]	ZHU BingLin, YU JiaLi, CHEN JiaYue, TIAN Yuan, WAN Yuan, LIU ChenYang, WANG XiaoYu, WANG MiaoLi, CHENG Gong. Cloning, Expression Characterization, and Functional Analysis of the Snail1 in Qinchuan Cattle and Its Impact on Proliferation of Bovine Adipocytes [J]. Scientia Agricultura Sinica, 2024, 57(13): 2674-2686.
[8]	YIN YanDie, YANG YanMei, FU QiChun, WANG Qin, LI YongQing, DUAN JinFeng, LIU YuZhu, WANG QiaoMei, HU XianQi. The Effects of Potato Root Exudates on the Hatching and Chemotaxis of Globodera rostochiensis and Verification of Exogenous Acid Substances [J]. Scientia Agricultura Sinica, 2024, 57(11): 2161-2175.
[9]	ZHANG HaiQing, ZHANG HengTao, GAO QiMing, YAO JiaLong, WANG YaRong, LIU ZhenZhen, MENG XiangPeng, ZHOU Zhe, YAN ZhenLi. Transcriptome Analysis for Screening Key Genes Related to Regulating Branching Ability in Apple [J]. Scientia Agricultura Sinica, 2024, 57(10): 1995-2009.
[10]	XIAO Tao, LI Hui, LUO Wei, YE Tao, YU Huan, CHEN YouBo, SHI YuShi, ZHAO DePeng, WU Yun. Screening of Candidate Genes for Green Shell Egg Shell Color Traits in Chishui Black Bone Chicken Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2023, 56(8): 1594-1605.
[11]	SUN YanFa, WU Qiong, LIN RuLong, CHEN HongPing, GAN QiuYun, SHEN Yue, WANG YaRu, XUE PengFei, CHEN FeiFan, LIU JianTao, ZHOU ChenXin, LAN ShiShi, PAN HaoZhe, DENG Fan, YUE Wen, JIANG XiaoBing, LI Yan. Genome-Wide Association Study of Egg Quality Traits in Longyan Shan-Ma Duck [J]. Scientia Agricultura Sinica, 2023, 56(3): 572-586.
[12]	CHEN JinRong, LÜ ZiJian, FAN LiSha, YOU Qian, LI Tao, GONG Chao, SUN GuangWen, LI ZhiLiang, SUN BaoJuan. Analysis of Genetic Effect of Fruit Color Controlled by Epistatic Genes in Eggplant [J]. Scientia Agricultura Sinica, 2023, 56(23): 4729-4741.
[13]	GAO GuangLiang, ZHANG KeShan, ZHAO XianZhi, XU GuoYang, XIE YouHui, ZHOU Li, ZHANG ChangLian, WANG QiGui. Identification of Molecular Markers Associated with Goose Egg Quality Through Genome-Wide Association Analysis [J]. Scientia Agricultura Sinica, 2023, 56(19): 3894-3904.
[14]	CHEN Qiu, HUANG JingJing, WANG ZhePeng. Establishment of Quantization Method and Genetic Basis Analysis of Brown Eggshell Color in the Lüeyang Black-Boned Chicken [J]. Scientia Agricultura Sinica, 2023, 56(17): 3452-3460.
[15]	GAO LiBing, CHEN Gang, WANG Jing, QI GuangHai, ZHANG HaiJun, QIU Kai, WU ShuGeng. Effects of Different Caging Regimes on Egg Yolk Flavor of Laying Hens [J]. Scientia Agricultura Sinica, 2023, 56(13): 2609-2619.