基于TMT蛋白质组学技术分析蝗虫微孢子虫感染飞蝗后的差异蛋白

doi:10.3864/j.issn.0578-1752.2024.24.005

Abstract

Abstract:

【Objective】The objective of this study is to determine differentially expressed proteins in Locusta migratoria before and after Paranosema locustae infection by using tandem mass tag (TMT) quantitative proteomics techniques, screen differentially expressed immune and metabolic related proteins, and to explore the pathogenic mechanism of P. locustae, so as to provide a scientific basis for better use of P. locustae to control locusts in the future. 【Method】The healthy nymphs obtained by laboratory incubation were inoculated with 5 µL of 1×10⁶ spores/mL P. locustae. The uninfected nymphs were used as the control group and fed under the same conditions as the infected group. The hemolymph of L. migratoria was taken as a sample. TMT technique was used to analyze the quantitative proteomics of the L. migratoria hemolymph in the infected group and the control group, and the differential proteins were identified. The biological process, molecular function and cellular component of differential proteins were analyzed by the Gene Ontology (GO) method. The differential proteins metabolic pathways were annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway website. 【Result】A total of 128 proteins were significantly different in abundance between the infected group and the control group, of which 66 proteins were up-regulated and 62 proteins were down-regulated in the infected group. GO analysis showed that the differential proteins were mainly involved in metabolic processes and were mainly distributed in cells. KEGG analysis showed that 16 proteins were significantly enriched in five pathways. Immune-related proteins, including six glutathione S-transferases (GSTs), one superoxide dismutase (SOD), four heat shock proteins (HSPs) and two peroxide dismutases (PODs), were significantly changed. Besides, metabolism-related proteins, including six glycometabolism-related proteins, two amino acids and one lipid metabolism-related proteins, were significantly changed. 【Conclusion】There were significant differences in the protein levels of L. migratoria before and after infection with P. locustae. Several differentially expressed proteins with different functions were screened, which were mainly distributed in cells. Immune-related proteins such as GST, HSP, SOD and POD were significantly up-regulated, indicating that immune and stimulus response-related proteins play an important role in the immune defense of locust hosts. The significant increase in metabolism-related proteins suggests that P. locustae infection promotes L. migratoria metabolism and provides energy for its proliferation.

Key words: Paranosema locustae, Locusta migratoria, hemolymph, proteomics, immune-related protein, metabolism-related protein

ZHANG HuiHui, KANG HanYe, LIU Hui, ZHANG JinRui, HUO Fan, GUO WeiQi, YE XiaoFang, JI Rong, HU HongXia. Differentially Expressed Proteins Analysis of Locusta migratoria Infected by Paranosema locustae Based on TMT Proteomics Technique[J].Scientia Agricultura Sinica, 2024, 57(24): 4884-4893.

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References 39

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通路 Pathway	P值 P value	通路号 Pathway ID
过氧化物酶体Peroxisome	0.015	ko04146
糖酵解Glycolysis	0.035	ko00010
核苷酸糖的生物合成 Biosynthesis of nucleotide sugars	0.048	ko01250
HIF-1信号通路 HIF-1 signaling pathway	0.048	ko04066
内吞作用Endocytosis	0.048	ko04144

蛋白类型 Protein type	登录号 Accession number	蛋白名称 Protein name	上调/下调表达倍数 Ratio of up/down expression
谷胱甘肽S-转移酶 Glutathione S-transferase	E7BTM5	GST-1	1.67↑
	A0A6G7K2Y6	GST-2	1.78↑
	V9Q4G7	GST-3	1.46↑
	V9Q315	GST-4	1.42↑
	F4YUJ0	GST-5	1.41↑
	A0A140DD58	GST-6	1.36↑
超氧化物歧化酶Superoxide dismutase	G9C5D1	SOD-1	1.78↑
热休克蛋白 Heat shock protein	G9C5E1	HSP-1	1.78↑
	Q0ZLZ4	HSP-2	1.43↑
	Q6SXP5	HSP-3	1.31↑
	Q6WAW3	HSP-4	1.20↑
过氧化物酶 Peroxide dismutase	A0A1B3PEJ4	POD-1	1.32↑
过氧化物酶 Peroxide dismutase	A0A7U0QRH0	POD-2	1.51↑

蛋白类型 Protein type	登录号 Accession number	蛋白名称 Protein name	上调/下调表达倍数 Ratio of up/down expression
糖代谢 Glycometabolism	C0KJJ7	己糖激酶Hexokinase	1.35↑
	C0KJJ8	磷酸葡萄糖变位酶Phosphoglucomutase	1.37↑
	G9C5D5	果糖二磷酸醛缩酶，I类Fructose-bisphosphate aldolase, class I	2.03↑
	O96558	甘油醛-3-磷酸脱氢酶（磷酸化） Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)	1.55↑
	H8YU84	甘油醛-3-磷酸脱氢酶Glyceraldehyde-3-phosphate dehydrogenase	1.46↑
	A8D372	海藻糖-6-磷酸合酶Trehalose-6-phosphate synthase	1.42↑
氨基酸代谢 Amino acid metabolism	G9C5D9	谷氨酰胺合成酶Glutamine synthetase	1.53↑
氨基酸代谢 Amino acid metabolism	A0A290WIX5	苯丙氨酸羟化酶Phenylalanine hydroxylase	1.41↑
脂代谢 Lipid metabolism	P10762	脂载蛋白Apolipophorin-3b	2.31↑

Differentially Expressed Proteins Analysis of Locusta migratoria Infected by Paranosema locustae Based on TMT Proteomics Technique

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