绿盲蝽G蛋白偶联受体激酶2基因（AlGRK2）的表达分析及在绿盲蝽生长发育中的功能

doi:10.3864/j.issn.0578-1752.2021.22.009

Abstract

Abstract:

【Objective】The objective of this study is to clone the full-length cDNA of the G protein-coupled receptor kinase 2 (AlGRK2) in Apolygus lucorum, clarify its expression profiles and the effect of exogenous ecdysterone hormone 20E on the expression of AlGRK2, analyze the role of AlGRK2 in the growth and development of A. lucorum, and to provide a preliminary research basis for further study of its function in the ecdysone signaling transduction pathway. 【Method】The AlGRK2 was cloned and obtained by RACE method. Using the qRT-PCR method, the relative expression levels of AlGRK2 in different days (1-16 day-old) and tissues (head, thorax, wing, leg, midgut, ovary and fat body) in female adults in A. lucorum were determined. Finally, the response of the AlGRK2 mRNA expression in A. lucorum after exogenous 20E induction and RNAi treatment and their effects on the main parameters (development progress, nymphs weight and adult emergence rate) in the growth and development of the A. lucorum were analyzed.【Result】The full length of AlGRK2 cDNA is 2 715 bp, and ORF is 2 106 bp, which encodes 701 amino acids. ExPASy predicts that the protein molecular weight is 80.2 kD and the theoretical isoelectric point is 6.56. Protein structure analysis shows that AlGRK2 contains 4 domains: G protein signalling domain (RGS, 54-175 aa), serine/threonine protein kinases domain (S-TKc, 191-454 aa), extension to Ser/Thr-type protein kinases (S-TK-X, 455-534 aa) and pleckstrin homology domain (PH, 558-655 aa), and the PH domain is a typical protein feature in the GRK2 subtype. Phylogenetic analysis showed that GRK2 in A. lucorum had the closest genetic relationship with Halyomorpha halys GRK2. qRT-PCR results showed that AlGRK2 was expressed in 1-day-old to 16-day-old nymphs of A. lucorum, and the mRNA expression showed a fluctuating downward pattern. The expression level of AlGRK2 was higher in the initial instar, but decreased significantly in the last instar of A. lucorum. The AlGRK2 was highly expressed in the ovary and fat body of female adults and less expressed in the thorax and leg. After exogenous 20E treatment, the expression of AlGRK2 was significantly down-regulated in nymphs at the 1-day-old and 3-day-old. The relative expression of AlGRK2 in all tissues of females was up-regulated after treating with 20E which compared with ethanol control, especially in ovary and fat body. In contrast, the expression of AlGRK2 treated with U73122 (PLC inhibitor) was down-regulated. Compared with ethanol control, the developmental duration of nymph, the body weight of the last instar nymph and the emergence rate of adult in A. lucorum were significantly decreased after 20E treatment. On the contrary, the development duration of nymph stage in U73122 treatment group was significantly prolonged. In addition, compared with the dsGFP group, AlGRK2 expression level of A. lucorum was significantly decreased after injection of dsAlGRK2, and nymphs mortality and developmental stages were significantly increased, while adult emergence rate and weight of the 5th instar nymph were significantly decreased. 【Conclusion】The expression profile of AlGRK2 in A. lucorum showed the specificity of the developmental stage and the tissue. Exogenous 20E inhibitor and RNAi treatment can inhibit the expression of AlGRK2, and have adverse effects on the growth and development of A. lucorum, such as delaying the development progress of A. lucorum, reducing the weight of 5th instar nymph and adult emergence rate.

Key words: Apolygus lucorum, G protein-coupled receptor kinase 2 (GRK2), ecdysterone hormone, gene cloning, expression profile, RNAi

TAN YongAn,JIANG YiPing,ZHAO Jing,XIAO LiuBin. Expression Profile of G Protein-Coupled Receptor Kinase 2 Gene (AlGRK2) and Its Function in the Development of Apolygus lucorum[J].Scientia Agricultura Sinica, 2021, 54(22): 4813-4825.

Figures/Tables 9

Table 1

Fig. 1

Fig. 2

Fig. 3

Table 2

Fig. 4

Fig. 5

Fig. 6

Fig. 7

References 47

[1]	陆宴辉, 吴孔明. 棉花盲椿象及其防治. 北京: 金盾出版社, 2008.
	LU Y H, WU K M. The Cotton Mirids and Its Control. Beijing: Jindun Publishing House, 2008. (in Chinese)
[2]	LU Y H, WU K M, JIANG Y Y, XIAO B, LI P, FENG H Q, WYCKHUYS K A G, GUO Y Y. Mirid bug outbreaks in multiple crops correlated with wide-scale adoption of Bt cotton in China. Science, 2010, 328(5982): 1151-1154. doi: 10.1126/science.1187881
[3]	PAN H S, LIU B, LU Y H, WYCKHUYS K A G. Seasonal alterations in host range and fidelity in the polyphagous mirid bug, Apolygus lucorum (Heteroptera: Miridae). PLoS ONE, 2015, 10(2): e0117153. doi: 10.1371/journal.pone.0117153
[4]	COSTANTINO B F B, BRICHE D K, ALEXANDRE K, SHEN K, MERRIAM J R, ANTONIEWSKI C, CALLENDER J L, HENRICH V C, PRESENTE A, ANDRES A J. A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila. PLoS Genetics, 2008, 4(6): e1000102. doi: 10.1371/journal.pgen.1000102
[5]	CHEN C H, PAN J, DI Y Q, LIU W, HOU L, WANG J X, ZHAO X F. Protein kinase C delta phosphorylates ecdysone receptor B1 to promote gene expression and apoptosis under 20-hydroxyecdysone regulation. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(34): E7121-E7130.
[6]	谭永安, 肖留斌, 郝德君, 赵静, 孙洋, 柏立新. 绿盲蝽AlEcR-A的单克隆抗体制备及在外源20E诱导下的应答. 中国农业科学, 2017, 50(1): 86-93.
	TAN Y A, XIAO L B, HAO D J, ZHAO J, SUN Y, BAI L X. Preparation of monoclonal antibody against AlEcR-A protein and its response induced by exogenous 20-hydroxyecdysone in Apolygus lucorum. Scientia Agricultura Sinica, 2017, 50(1): 86-93. (in Chinese)
[7]	LIU W, CAI M J, WANG J X, ZHAO X F. In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology, 2014, 155(5): 1738-1750. doi: 10.1210/en.2013-2020
[8]	谭永安. 磷脂酶C、E75在绿盲蝽蜕皮激素信号传导中的功能分析[D]. 南京: 南京林业大学, 2019.
	TAN Y A. The functional of phospholipase C and E75 in 20E pathway of Apolygus lucorum[D]. Nanjing: Nanjing Forestry University, 2019. (in Chinese)
[9]	PENELA P, RIBAS C, MAYOR F. Mechanisms of regulation of the expression and function of G protein-coupled receptor kinases. Cellular Signalling, 2003, 15(11): 973-981. doi: 10.1016/S0898-6568(03)00099-8
[10]	ZHANG X, KIM K M. Multifactorial regulation of G protein-coupled receptor endocytosis. Biomolecules and Therapeutics, 2017, 25(1): 26-43. doi: 10.4062/biomolther.2016.186
[11]	GUREVICH E V, TEAMER J J G, MUSHEGIAN A, GUREVICH V V. G protein-coupled receptor kinases: More than just kinases and not only for GPCRs. Pharmacology and Therapeutics, 2012, 133(1): 40-69. doi: 10.1016/j.pharmthera.2011.08.001
[12]	KOMOLOV K E, BENOVIC J L. G protein-coupled receptor kinases: Past, present and future. Cellular Signalling, 2018, 41: 17-24. doi: 10.1016/j.cellsig.2017.07.004
[13]	INGLESE J, KOCH W J, CARON M G, LEFKOWITZ R J. Isoprenylation in regulation of signal transduction by G-protein- coupled receptor kinases. Nature, 1992, 359(6391): 147-150. doi: 10.1038/359147a0
[14]	BENOVIC J L, MAYOR F, SOMERS R L, CARON M G, LEFKOWITZ R J. Light-dependent phosphorylation of rhodopsin by β-adrenergic receptor kinase. Nature, 1986, 321(6073): 869-872. doi: 10.1038/321869a0
[15]	BOEKHOFF I, INGLESE J, SCHLEICHER S, KOCH W J, LEFKOWITZ R J, BREER H. Olfactory desensitization requires membrane targeting of receptor kinase mediated by βγ-subunits of heterotrimeric G proteins. The Journal of Biological Chemistry, 1994, 269(1): 37-40. doi: 10.1016/S0021-9258(17)42306-4
[16]	OPPERMANN M, DIVERSE-PIERLUISSI M, DRAZNER M H, DYER S L, FREEDMAN N J, PEPPEL K C, LEFKOWITZ R J. Monoclonal antibodies reveal receptor specificity among G-protein- coupled receptor kinases. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(15): 7649-7654.
[17]	STOFFEL R H, RANDALL R R, PREMONT R T, LEFKOWITZ R J, INGLESE J. Palmitoylation of G protein-coupled receptor kinase, GRK6. Lipid modification diversity in the GRK family. The Journal of Biological Chemistry, 1994, 269(45): 27791-27794. doi: 10.1016/S0021-9258(18)46852-4
[18]	PREMONT R T, MACRAE A D, STOFFEL R H, CHUNG N J, PITCHER J A, AMBROSE C, INGLESE J, MACDONALD M E, LEFKOWITZ R J. Characterization of the G protein-coupled receptor kinase GRK4. Identification of four splice variants. The Journal of Biological Chemistry, 1996, 271(11): 6403-6410. doi: 10.1074/jbc.271.11.6403
[19]	ZHAO W L, WANG D, LIU C Y, ZHAO X F. G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling. Scientific Reports, 2016, 6: 29205. doi: 10.1038/srep29205
[20]	GARCIA-GUERRA L, VILA-BEDMAR R, CARRASCO-RANDO M, CRUCES-SANDE M, MARTÍN M, RUIZ-GÓMEZ A, RUIZ-GÓMEZ M, LORENZO M, FERNÁNDEZ-VELEDO S, MAYOR F, MURGA C, NIETO-VÁZQUEZ I. Skeletal muscle myogenesis is regulated by G protein-coupled receptor kinase 2. Journal of Molecular Cell Biology, 2014, 6(4): 299-311. doi: 10.1093/jmcb/mju025
[21]	KUBO I, KLOCKE J A, ASANO S. Insect ecdysis inhibitors from the East African medicinal plant Ajuga remota (Labiatae). Agricultural and Biological Chemistry, 1981, 45(8): 1925-1927.
[22]	BLACKFORD M J P, DINAN L. The effects of ingested 20- hydroxyecdysone on the larvae of Aglais urticae, Inachis io, Cynthia cardui (Lepidoptera: Nymphalidae) and Tyria jacobaeae (Lepidoptera: Arctiidae). Journal of Insect Physiology, 1997, 43(4): 315-327. pmid: 12769893
[23]	KLEIN R R, BOURDON D M, COSTALES C L, WAGNER C D, WHITE W L, WILLIAMS J D, HICKS S N, SONDEK J, THAKKER D R. Direct activation of human phospholipase C by its well known inhibitor U73122. The Journal of Biological Chemistry, 2011, 286(14): 12407-12416. doi: 10.1074/jbc.M110.191783
[24]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 2001, 25: 402-408. doi: 10.1006/meth.2001.1262
[25]	KARLSON P. On the hormonal control of insect metamorphosis. A historical review. The International Journal of Developmental Biology, 1996, 40(1): 93-96.
[26]	LANOT R, THIEBOLD J, COSTET-CORIO M F, BENVENISTE P, HOFFMANN J A. Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera). Developmental Biology, 1988, 126(1): 212-214. doi: 10.1016/0012-1606(88)90255-2
[27]	LOEB M J, DE LOOF A, GELMAN D B, HAKIM R S, JAFFE H, KOCHANSKY J P, MEOLA S M, SCHOOFS L, STEEL C, VAFOPOULOU X, WAGNER R M, WOODS C W. Testis ecdysiotropin, an insect gonadotropin that induces synthesis of ecdysteroid. Archives of Insect Biochemistry and Physiology, 2010, 47(4): 181-188. doi: 10.1002/(ISSN)1520-6327
[28]	DAIMON T, UCHIBORI M, NAKAO H, SEZUTSU H, SHINODA T. Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(31): E4226-E4235.
[29]	MALAUSA T, SALLES M, MARQUET V, GUILLEMAUD T, ALLA S, MARION-POLL F, LAPCHIN L. Within-species variability of the response to 20-hydroxyecdysone in peach-potato aphid (Myzus persicae Sulzer). Journal of Insect Physiology, 2006, 52(5): 480-486. doi: 10.1016/j.jinsphys.2006.01.007
[30]	SUN L J, LIU Y J, SHEN C P. The effects of exogenous 20-hydroxyecdysone on the feeding, development, and reproduction of Plutella xylostella (Lepidoptera: Plutellidae). Florida Entomologist, 2015, 98(2): 606-612. doi: 10.1653/024.098.0233
[31]	MARINISSEN M J, GUTKIND J S. G-protein-coupled receptors and signaling networks: Emerging paradigms. Trends in Pharmacological Sciences, 2001, 22(7): 368-376. doi: 10.1016/S0165-6147(00)01678-3
[32]	BELMONTE S L, BLAXALL B C. G protein coupled receptor kinases as therapeutic targets in cardiovascular disease. Circulation Research, 2011, 109(3): 309-319. doi: 10.1161/CIRCRESAHA.110.231233
[33]	KANG J H, TOITA R, KAWANO T, MURATA M, ASAI D. Design of substrates and inhibitors of G protein-coupled receptor kinase 2 (GRK2) based on its phosphorylation reaction. Amino Acids, 2020, 52(6): 863-870. doi: 10.1007/s00726-020-02864-x
[34]	APPLE R T, FRISTROM J W. 20-Hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes. Developmental Biology, 1991, 146(2): 569-582. doi: 10.1016/0012-1606(91)90257-4
[35]	DOCTOR J, FRISTRIM D, FRISTRIM J W. The pupal cuticle of Drosophila: Biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone. The Journal of Cell Biology, 1985, 101(1): 189-200. doi: 10.1083/jcb.101.1.189
[36]	SALCEDO A, MAYOR F, PENELA P. Mdm2 is involved in the ubiquitination and degradation of G-protein-coupled receptor kinase 2. The EMBO Journal, 2006, 25(20): 4752-4762. doi: 10.1038/sj.emboj.7601351
[37]	HUANG J A, NALLI A D, MSHAVADI S, KUMAR D P, MURTHY K S. Inhibition of Gα_i activity by Gβγ is mediated by PI 3-kinase-γ- and cSrc- dependent tyrosine phosphorylation of Gα_i and recruitment of RGS12. American Journal of Physiology Gastrointestinal and Liver Physiology, 2014, 306(9): G802-G810. doi: 10.1152/ajpgi.00440.2013
[38]	WALDSCHMIDT H V, HOMAN K T, CRUZ-RODRIGUEZ O, CATO M C, WANINGER-SARONI J, LARIMORE K M, CANNAVO A, SONG J, CHEUNG J Y, KIRCHHOFF P D, KOCH W J, TESMER J J G, LARSEN S D. Structure-based design, synthesis and biological evaluation of highly selective and potent G protein-coupled receptor kinase 2 inhibitors. Journal of Medicinal Chemistry, 2016, 59(8): 3793-3807. doi: 10.1021/acs.jmedchem.5b02000
[39]	JABER M, KOCH W J, ROCKMAN H, SMITH B, BOND R A, SULIK K K, ROSS J, LEFKOWITZ R J, CARON M G, GIROS B. Essential role of β-adrenergic receptor kinase 1 in cardiac development and function. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(23): 12974-12979.
[40]	PHILIPP M, FRALISH G B, MEIONI A R, CHEN W, MACINNES A W, BARAK L S, CARON M G. Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase. Molecular Biology of the Cell, 2008, 19(12): 5478-5489. doi: 10.1091/mbc.e08-05-0448
[41]	WANG J J, LUO J S, ARYAL D K, WETSEL W C, NASS R, BENOVIC J L. G protein-coupled receptor kinase-2 (GRK-2) regulates serotonin metabolism through the monoamine oxidase AMX-2 in Caenorhabditis elegans. The Journal of Biological Chemistry, 2017, 292(14): 5943-5956. doi: 10.1074/jbc.M116.760850
[42]	ISAAC R E, REES H H. Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria. The Biochemical Journal, 1984, 221(2): 459-464. doi: 10.1042/bj2210459
[43]	JIANG X, YANG P, MA L. Kinase activity-independent regulation of cyclin pathway by GRK2 is essential for zebrafish early development. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(25): 10183-10188.
[44]	杨中侠, 文礼章, 吴青君, 王少丽, 徐宝云, 张友军. RNAi技术在昆虫功能基因研究中的应用进展. 昆虫学报, 2008, 51(10): 1077-1082.
	YANG Z X, WEN L Z, WU Q J, WANG S L, XU B Y, ZHANG Y J. Application of RNA interference in studying gene functions in insects. Acta Entomologica Sinica, 2008, 51(10): 1077-1082. (in Chinese)
[45]	MCFARLANE M, LAURETI M, LEVEE T, TERRY S, KOHL A, PONDEVILLE E. Improved transient silencing of gene expression in the mosquito female Aedes aegypti. Insect Molecular Biology, 2021, 30(3): 355-365. doi: 10.1111/imb.v30.3
[46]	ZHU J H, LIU X Q, ZHU K M, ZHOU H Y, LI L, LI Z X, QIN W W, HE Y P. Knockdown of TRPV genes affects the locomotion and feeding behavior of Nilaparvata lugens (Hemiptera: Delphacidae). Journal of Insect Science, 2020, 20(1): 9.
[47]	黄海山. 家蚕脂动激素受体信号转导机制研究[D]. 杭州: 浙江大学, 2010.
	HUANG H S. The mechanism of signal transduction of adipokinetic hormone receptor in Bombyx mori[D]. Hangzhou: Zhejiang University, 2010. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

目的 Purpose	引物名称 Primer name	引物序列Primer sequence (5′ to 3′)
克隆Cloning	AlGRK-F	AGYGTNMGVAGYGTNATGCA
	AlGRK-R	TCVGCKGCRTARAAYTTCAT
	5′-AlGRK2-F	TGAAGAGAAGGAATCCCAGA
	5′-AlGRK2-R	ACTTCGTTCTTCTTTTCGAG
	3′-AlGRK2-F	GCTGGCACTCAACGAAAGGATCAT
	3′-AlGRK2-R	GTATGACTTACGCCTTCCACACGC
dsRNA	AlGRK2-F	TTCCCGACTCCTTCTCATC
	AlGRK2-R	TTTCCGTTTCTGCTCCG
	AlGRK2-T7F	TAATACGACTCACTATAGGGTTCCCGACTCCTTCTCATC
	AlGRK2-T7R	TAATACGACTCACTATAGGGTTTCCGTTTCTGCTCCG
	GFP-F	CACAAGTTCAGCGTGTCCG
	GFP-R	CACCTTGATGCCGTTC
	GFP-T7F	TAATACGACTCACTATAGGGCACAAGTTCAGCGTGTCCG
	GFP-T7R	TAATACGACTCACTATAGGGCACCTTGATGCCGTTC
qRT-PCR	AlGRK2-QF	AGGAGCGTGATGCACAAATA
	AlGRK2-QR	CGCAGTAGTCCTTGAAGAGAAG
	β-Actin-QF	ACCTGTACGCCAACACCGT
	β-Actin-QR	TGGAGAGAGAGGCGAGGAT

处理 Treatment	若虫历期Developmental period of nymphs (d)
处理 Treatment	1龄1st instar	2龄2nd instar	3龄3rd instar	4龄4th instar	5龄5th instar	若虫期Nymph
乙醇Ethanol (CK)	2.62±0.07bc	3.51±0.07b	3.60±0.07b	3.55±0.07a	3.77±0.09a	17.04±0.18b
20E	2.42±0.07c	3.17±0.05c	3.02±0.09c	2.96±0.04b	3.38±0.08b	14.94±0.19c
U73122	2.93±0.09a	3.85±0.05a	3.80±0.06a	3.67±0.07a	3.83±0.09a	18.09±0.18a
20E+U73122	2.79±0.07ab	3.66±0.07b	3.79±0.06ab	3.62±0.07a	3.89±0.11a	17.77±0.21a

Expression Profile of G Protein-Coupled Receptor Kinase 2 Gene (AlGRK2) and Its Function in the Development of Apolygus lucorum

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 9

References 47

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHANG Qi, CHEN ErHu, SUN DeHong, TANG PeiAn. Relationship Between Glutathione S-Transferase Genes CfGSTe1 and CfGSTd1 and Ethyl Formate Tolerance in Cryptolestes ferrugineus [J]. Scientia Agricultura Sinica, 2026, 59(5): 1008-1019.
[2]	YAN WenYing, ZHANG YuanZhen, WU HongXin, PANG Rui, CHEN ZePeng, JIN FengLiang, XU XiaoXia. Construction of an RNAi-Enhanced Metarhizium anisopliae Targeting PxGNBP3 and Its Immunoregulatory Mechanism in Plutella xylostella [J]. Scientia Agricultura Sinica, 2026, 59(3): 556-574.
[3]	TENG MengXin, XU Ya, HE Jing, WANG Qi, QIAO Fei, LI JingYang, LI XinGuo. Identification and Functional Analysis of Ca²⁺-ATPase Gene Family in Banana [J]. Scientia Agricultura Sinica, 2025, 58(7): 1418-1433.
[4]	CHEN ErHu, TANG JingJie, HU ShunJie, TANG PeiAn. The Roles of Heat Shock Protein Genes CfHsp70-1 and CfHsp70-2 in Enhancing the High-Temperature Tolerance after Heat Acclimation in Cryptolestes ferrugineus [J]. Scientia Agricultura Sinica, 2025, 58(5): 918-928.
[5]	ZHENG YaQin, LIU XueQing, WU SiWen, TANG XiaoYan, YANG DanNi, WANG YongKang, AHMAD Aftab, KHAN Afrsyab, WANG ChengGang, CHEN GuoHu. Cloning and Expression of BcDET2 Gene and Functional of Its Regulatory Effect on Bolting and Flowering in Wucai (Brassica campestris L.) [J]. Scientia Agricultura Sinica, 2025, 58(5): 991-1003.
[6]	ZOU PeiYi, LIU MeiYan, WANG Ying, LI RanHong. Cloning and Functional Study of AkNAC2 from Actinidia kolomikta [J]. Scientia Agricultura Sinica, 2025, 58(19): 3985-3999.
[7]	ZHANG ShuHong, GAO FengJu, WU QiuYing, JI JingXin, ZHANG YunFeng, XU Ke, GU ShouQin, FAN YongShan. Cloning and Expression Analysis of Heat Shock Protein HSP 9/12 Genes in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2025, 58(18): 3648-3663.
[8]	XIAO ZhuoDan, QIAO JiaZheng, GAO YuLan, SHANG ZhangYin, LIU Huai, WANG Jia. Silencing of Cytochrome P450 Genes CYP6CY53 and CYP302A1 in Aphis craccivora Enhances the Sensitivity to Flonicamid [J]. Scientia Agricultura Sinica, 2025, 58(18): 3664-3675.
[9]	YANG WenJuan, GAO JiaCheng, WANG YanTing, LI Yan, GUO Ming, WANG JunCheng, MENG YaXiong, WANG HuaJun, SI ErJing. Function of Effector Pg00778 Regulation on the Pathogenicity of Pyrenophora graminea to Barley [J]. Scientia Agricultura Sinica, 2025, 58(15): 3020-3035.
[10]	LÜ ShuWei, TANG Xuan, LI Chen. Research Progress on Seed Shattering of Rice [J]. Scientia Agricultura Sinica, 2025, 58(1): 1-9.
[11]	CHEH ErHu, YUAN GuoQing, CHEN Yan, CHEN MengQiu, SUN ShengYuan, TANG PeiAn. Mitochondrial Protein-Coding Genes Nad5, Nad6 and Atp6 are Involved in Phosphine Resistance of Cryptolestes ferrugineus [J]. Scientia Agricultura Sinica, 2024, 57(9): 1722-1733.
[12]	LUO LiDan, CHEN JiaMing, AN Qi, LIU Lei, SUN QinZhe, LIU Huan, WANG SenShan, SONG LiWen. Effects of Extreme High Temperature on Trehalose Content and Trehalose Transporter Gene in Tetranychus truncatus [J]. Scientia Agricultura Sinica, 2024, 57(6): 1091-1101.
[13]	GUAN ZhiLin, JIN FengWei, LIU TingTing, WANG Yi, TAN YingYing, YANG ChunHui, LI RuiTong, WANG Bo, LIU KeDe, DONG Yun. Genetic Analysis and Gene Mapping of Glossy Leaf in Brassica napus [J]. Scientia Agricultura Sinica, 2024, 57(4): 650-662.
[14]	ZHAO YiYan, GUO HongFang, LIU WeiMin, ZHAO XiaoMing, ZHANG JianZhen. Effects of Apolipophorin on Ovarian Development and Lipid Deposition in Locusta migratoria [J]. Scientia Agricultura Sinica, 2024, 57(4): 711-720.
[15]	FENG WenMi, ZHOU FangXue, YU Zhe, MOU KeXin, JING Yan, LI HaiYan. Cloning and Functional Analysis of GmRHF1 Gene Against Soybean Mosaic Virus [J]. Scientia Agricultura Sinica, 2024, 57(23): 4632-4643.