花铃期棉花黄萎病抗病与感病品种对 土壤细菌群落结构的影响

doi:10.3864/j.issn.0578-1752.2020.05.007

Abstract

Abstract:

【Objective】The objective of this study is to research soil bacterial community structure of cotton verticillium wilt resistant and susceptible varieties at flowering and boll stage, understand the relationship between soil bacterial community structure and soil physicochemical properties, and to provide a theoretical basis for monitoring of cotton verticillium wilt and green ecological control. 【Method】The susceptible (EJ) and resistant (J863) varieties were used in field plot experiments. The ITS gene copy number of Verticillium dahliae and soil bacterial community structure at different flowering and boll stages (flowering, late flowering, and boll-forming stages) were studied by real-time fluorescence quantitative PCR (real-time PCR) and high throughput sequencing (Illumina MiSeq), respectively. The redundancy analysis (RDA) was used to determine the correlation between bacterial community structure and soil physicochemical properties. 【Result】There were different degrees of relevance between the occurrence of verticillium wilt and the ITS gene copy number of V. dahliae in soil. The incidence and disease index of susceptible variety EJ were positively correlated with the ITS gene copy number of V. dahliae, while those of resistant variety J863 were not significantly correlated with the ITS gene copy number of V. dahliae. The ITS gene copy number of V. dahliae in soil of resistant variety J863 was lower than that of susceptible variety EJ at late flowering and boll-forming stages. The high throughput sequencing analysis showed that the bacterial richness indices (Chao1 and ACE) of resistant variety J863 were higher than those of susceptible variety EJ at flowering and boll-forming stages. The principal component analysis showed that there were differences in soil bacterial community structure between resistant and susceptible varieties and at different flowering and boll stages. At phylum level, the average relative abundance of some dominant bacteria in susceptible variety EJ was lower than that of resistant variety J863, such as Actinobacteria, Gemmatimonadetes, Chloroflexi, Bacteroidetes, Nitrospirae, Patescibacteria, and Armatimonadetes, which was decreased by 16.38%, 4.05%, 2.25%, 6.58%, 7.10%, 20.60%, and 35.78%, respectively. At genus level, the average relative abundance of some dominant bacteria of susceptible variety EJ was lower than that of resistant variety J863, including Sphingomonas, Gemmatimonas, Bryobacter, Iamia, Pseudarthrobacter, Blastococcus, Rubrobacter, Nocardioides, Pontibacter, Streptomyces, Gemmatirosa, Micromonospora, and Solirubrobacter, which was decreased by 5.09%, 19.41%, 13.79%, 2.36%, 10.78%, 34.47%, 46.76%, 61.84%, 52.75%, 48.61%, 74.79%, 9.13%, and 26.42%, respectively. The redundancy analysis (RDA) showed that the bacterial community structure was affected by nitrate nitrogen (NO₃ ^--N), available phosphorus (AP), ammonium nitrogen (NH₄ ⁺-N), inorganic phosphorus (IP), pH and organic matter (OM). 【Conclusion】The correlation between the ITS gene copy number of V. dahliae in soil and the occurrence of cotton verticillium wilt of susceptible and resistant varieties is different, and the incidence of verticillium wilt in susceptible variety is positively correlated with the ITS gene copy number of V. dahliae in soil. The bacterial community structure of resistant variety is better than susceptible variety at flowering, late flowering, and boll-forming stages. There are some differences in the dominant bacterial community at different growth stages. The diversity, relative abundance and composition of bacteria in soil are affected by organic matter, pH, nitrogen type and available phosphorus. Meanwhile, the growth stage of cotton had a significant influence on the bacterial community structure in soil.

Key words: cotton verticillium wilt, Verticillium dahliae, disease resistance, flowering and boll stage, bacterial community structure, Illumina MiSeq, soil physicochemical properties

ZHAO WeiSong,GUO QingGang,LI SheZeng,WANG PeiPei,LU XiuYun,SU ZhenHe,ZHANG XiaoYun,MA Ping. Effect of Wilt-Resistant and Wilt-Susceptible Cotton on Soil Bacterial Community Structure at Flowering and Boll Stage[J].Scientia Agricultura Sinica, 2020, 53(5): 942-954.

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References 42

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指标 Index	鄂荆1号 EJ			冀863 J863
指标 Index	盛花期 Flowering stage	开花后期 Late flowering stage	结铃期 Boll-forming stage	盛花期 Flowering stage	开花后期 Late flowering stage	结铃期 Boll-forming stage
铵态氮NH₄⁺-N (mg·kg^-1)	59.20±1.32a	35.24±0.96b	65.35±2.11a	28.70±1.25b	22.71±1.05c	36.32±0.95a
硝态氮NO₃^--N (mg·kg^-1)	66.28±2.03a	4.74±1.41b	2.70±0.73c	45.77±1.49a	15.00±2.35b	7.31±0.93c
pH	7.98±0.04a	7.64±0.08b	7.65±0.03b	7.65±0.04a	7.68±0.02a	7.55±0.04a
速效磷AP (mg·kg^-1)	676.39±2.48b	625.76±1.53c	689.13±2.58a	624.07±2.05b	597.07±3.27c	656.84±2.55a
无机磷IP (μg·g^-1)	252.37±3.55b	365.93±2.49a	89.69±5.02c	277.60±3.27b	353.31±2.95a	100.95±2.63c
有机质OM (%)	0.45±0.06a	0.37±0.02b	0.34±0.05b	0.40±0.05a	0.31±0.01b	0.24±0.03c

品种 Variety	时期 Stage	测序 Reads	丰富度 Richness		均匀度Evenness
品种 Variety	时期 Stage	测序 Reads	Chao1指数 Chao1 index	ACE指数 ACE index	辛普森指数 Simpson index	香浓指数 Shannon index
鄂荆1号 EJ	盛花期Flowering	36808.00±1137.88a	2982.50±219.77b	3200.66±212.43b	0.9982±0.0001a	10.21±0.03a
	开花后期Late flowering	43256.67±2401.77b	3526.68±0.18c	3711.64±199.50b	0.9982±0.0001a	10.27±0.10a
	结铃期 Boll-forming	34437.33±1815.87a	2002.00±41.30a	2002.35±41.61a	0.9980±0.0001a	10.13±0.04a
冀863 J863	盛花期Flowering	39820.33±1893.73b	3292.79±46.52b	3460.79±154.20b	0.9977±0.0005a	10.20±0.14a
	开花后期Late flowering	39683.33±2082.20b	3330.36±101.99b	3470.68±113.89b	0.9979±0.0002a	10.12±0.12a
	结铃期 Boll-forming	36219.67±870.40a	2880.41±146.59a	3005.45±85.32a	0.9980±0.0001a	10.18±0.01a

Effect of Wilt-Resistant and Wilt-Susceptible Cotton on Soil Bacterial Community Structure at Flowering and Boll Stage

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