基于隶属函数分析的甘薯薯苗耐冷性基因型差异研究

doi:10.3864/j.issn.0578-1752.2019.17.002

Abstract

Abstract:

【Objective】 It is of great significance to explore the low temperature tolerance mechanism of sweet potato seedlings and identify the key factors affecting the low temperature tolerance of the seedlings for evaluation and screening of cold tolerance genetic resources of the seedlings and research on the mechanism of low temperature tolerance of sweet potatoes.【Method】 In order to investigate the cold tolerance of different varieties of sweet potato seedlings, 30 varieties of sweet potato seedlings were selected and tested under low temperature conditions of 4℃ in this study, their physiological indexes and antioxidant enzyme indexes were measured so as to observe the effects of low temperature stress on physiological characteristics of sweet potato seedlings. The low temperature tolerant sweet potato varieties were screened by the subordinate function analysis of the physiological characteristics of sweet potato seedlings.【Result】 Great difference of the physiological indexes of 30 different genotypes of sweet potato seedlings were found under low temperature stress. After low temperature stress the relative electrical conductivity and proline contents of all sweet potato seedlings were increased to different extents. The relative conductivity of Sushu 16 changed the most, up to 40.16%, and the most increased proline content was Liaoshu 15 up to 3.66 μg·g ^-1 FW; malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), Ascorbate peroxidase (APX) showed an upward trend; among the 30 varieties of potato seedlings, the most significant change in MDA content was Suyu 303; the SOD activity of all sweet potato seedlings increased, and the most variable SOD activity was Yushu 17. The change before and after stress was as high as 27.59 U·g ^-1 FW, but Ningzishu 1, Shangxuzi 1 and Ziluolan seedlings were in a state of wilting and death, and the change of SOD activity showed a negative value; the POD activity of all potato seedlings showed an upward trend. The highest change in POD activity was Gan 10-20, up to 29.21 U·(g·min) ^-1, and the change of POD content in Ziluolan was negative; the highest increase in CAT activity was Hongxiangjiao; The change of APX activity showed an upward trend, and the potato 25, Hongxiangjiao and Longzishu 6 greatest change, Pu 32, Shangshu 8, Ziluolan the minimum change; proline content is increased up to Liao 15, up to 3.66, It is 1.5 times that of Shangxuzi 1. Correlation analysis of physiological indexes of sweet potato seedlings showed that relative conductivity and MDA content were negatively correlated with cold tolerance of sweet potato seedlings. SOD, POD, CAT and APX activities were positively correlated with cold tolerance of sweet potato seedlings, proline content and cold tolerance of sweet potato seedlings. Sexuality is positively correlated.【Conclusion】 Comprehensive conductivity and antioxidant enzymes and proline indicators, according to membership function analysis. Different varieties of sweet potatoes were different in low temperature resistance. At the temperature of 4℃, the order of low temperature resistance of all sweet potatoes was: Longshu515>Gan10-20>Nanshu010>Hongxiangjiao>Guangshu87>Sushu8>Yushu17>Longzishu6>Yanshu25>Zheshu13>Yizhi138>Sweet potato 166-7>Qingzishu2>Xushu55-2>Liaoshu19>Liaoshu15>Longshu14>Xushu22>Shangshu19>Pushu32>Ningzishu2>Longshu9>Shangshu8>Xuzishu8>Hongdong>Sushu16>Taiwanyingou>Shangxuzishu1>Suyu303>Ziluolan.

Key words: sweet potato, low temperature stress, antioxidant, proline, membership function analysis

XU Shu,LI Ling,ZHANG SiMeng,CAO RuXia,CHEN LingLing,CUI Peng,Lü ZunFu,WU LieHong,LU GuoQuan. Evaluation of Genotype Differences of Cold Tolerance of Sweet Potato Seedlings by Subordinate Function Value Analysis[J].Scientia Agricultura Sinica, 2019, 52(17): 2929-2938.

Figures/Tables 4

Table 1

Membership values of different genotypes of sweet potato seedlings"

品种 Variety.	电导率 Conductivity	丙二醛 Malondialdehyde	超氧化物歧化酶 Superoxide dismutase	过氧化物酶 Peroxidase	过氧化氢酶 Catalase	抗坏血酸过氧化酶 Aseorbate peroxidase	脯氨酸 Proline	平均值 Average
龙薯515 Longshu 515	0.89	0.88	0.54	0.72	0.65	0.29	0.87	0.69
赣10-20 Gan 10-20	1.00	0.73	0.20	1.00	0.56	0.14	0.63	0.61
南薯010 Nanshu 010	0.95	0.81	0.30	0.23	1.00	0.21	0.57	0.58
红香蕉 Hongxiangjiao	0.87	0.38	0.11	0.80	0.14	0.86	0.91	0.58
广薯87 Guangshu 87	0.86	0.73	0.26	0.25	0.37	0.54	1.00	0.57
苏薯8号 Sushu 8	1.00	0.66	0.72	0.19	0.21	0.14	0.97	0.56
渝薯17 Yushu 17	0.88	0.80	0.61	0.18	0.07	0.50	0.79	0.55
龙紫薯6号 Longzishu 6	0.71	1.00	0.27	0.75	0.05	0.54	0.48	0.54
烟薯25 Yanshu 25	0.71	0.66	0.27	0.15	0.26	1.00	0.46	0.50
浙薯13 Zheshu 13	0.75	0.70	1.00	0.15	0.03	0.29	0.31	0.46
遗字138 Yizi 138	0.63	0.76	0.12	0.24	0.05	0.68	0.63	0.44
甘薯166-7 Ganshu 166-7	0.75	0.87	0.27	0.18	0.08	0.50	0.34	0.43
秦紫薯2号 Qinzishu 2	0.72	0.79	0.44	0.15	0.16	0.18	0.17	0.37
徐薯55-2 Xushu 55-2	0.60	0.65	0.49	0.31	0.02	0.07	0.32	0.35
辽薯19 Liaoshu 19	0.73	0.31	0.20	0.49	0.03	0.14	0.57	0.35
辽薯15 Liaoshu 15	0.57	0.47	0.26	0.38	0.39	0.07	0.25	0.34
龙薯14 Longshu 14	0.71	0.58	0.30	0.23	0.08	0.29	0.20	0.34
徐薯22 Xushu 22	0.47	0.42	0.12	0.14	0.07	0.39	0.69	0.33
商薯19 Shangshu 19	0.53	0.74	0.32	0.31	0.05	0.04	0.28	0.32
普薯32 Pushu 32	0.35	0.63	0.46	0.18	0.04	0.00	0.53	0.31
宁紫薯2号 Ningzishu 2	0.69	0.74	0.20	0.17	0.01	0.18	0.20	0.31
龙薯9号 Longshu 9	0.70	0.64	0.12	0.17	0.10	0.29	0.14	0.31
商薯8号 Shangshu 8	0.79	0.62	0.44	0.19	0.02	0.00	0.04	0.30
徐紫薯8号 Xuzishu 8	0.49	0.36	0.41	0.17	0.15	0.07	0.33	0.28
红东 Hongdong	0.60	0.59	0.12	0.12	0.00	0.18	0.17	0.25
苏薯16 Sushu 16	0.00	0.43	0.30	0.19	0.10	0.43	0.32	0.25
台湾英沟 Taiwanyinggou	0.59	0.31	0.12	0.47	0.08	0.18	0.00	0.25
商徐紫1号 Shangxuzi 1	0.41	0.18	0.30	0.19	0.17	0.18	0.12	0.22
苏渝303 Suyu 303	0.51	0.00	0.27	0.29	0.07	0.11	0.19	0.20
紫罗兰 Ziluolan	0.45	0.03	0.05	0.00	0.45	0.04	0.18	0.17

Table 1

Fig. 1

Fig. 2

Table 2

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Evaluation of Genotype Differences of Cold Tolerance of Sweet Potato Seedlings by Subordinate Function Value Analysis

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