Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (22): 4339-4351.doi: 10.3864/j.issn.0578-1752.2018.22.012

• HORTICULTURE • Previous Articles     Next Articles

Cloning and Function Analysis of Apple Gibberellin Oxidase Gene MdGA2ox8

LI FeiHong(),HOU YingJun,LI XueHan,YU XinYi,QU ShenChun()   

  1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095
  • Received:2018-06-04 Accepted:2018-07-16 Online:2018-11-16 Published:2018-11-16

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Abstract:

【Objective】The objective of this study is to clone the open reading frame (ORF) of a gibberellin 2-oxidase gene (gibberellin2-oxidase 8, GA2ox8) from apple cultivar ‘Sushuai’, to analyze its sequence characteristics and tissue expression specificity. The effects of overexpression of MdGA2ox8 on tobacco growth and development were studied in order to provide a theoretical reference for the functional analysis and application of the gene.【Method】The ORF region of MdGA2ox8 was cloned from apple by RT-PCR method. The amino acid sequence alignment and conserved domain analysis were performed by NCBI, DNAMAN and Pfam online software. The composition, theoretical molecular weight and isoelectric point (pI) of the amino acid were deduced by Expasy software online. The protein signal peptide and transmembrane domain were analyzed by SignalP and TMHMM Server V.2.0. The phylogenetic tree was constructed by a neighbor-joining (NJ) method using MEGA 7.0 program. The expression level in different tissues of apple was detected by RT-qPCR. To characterize the function of MdGA2ox8, MdGA2ox8 ORF driven by the 35S promoter was delivered into tobacco by Agrobacterium-mediated transformation approach. Transgenic plants with hygromycin resistance were obtained and identified by using GUS staining, genomic PCR, and RT-PCR. After transplanting, the plant height, internode length, blade aspect ratio, content of chlorophyll, and content of GA1, GA4 in tobacco were measured at the first flowering stage. The expression level of related genes in tobacco was analyzed by RT-qPCR.【Result】The ORF sequence of MdGA2ox8 obtained from ‘Sushuai’ is 1 122 bp in length, encoding a putative protein about 373 amino acids. The predicted molecular weight of MdGA2ox8 is 42.8 kD, the theoretical pI is 5.44 and the instability coefficient is 49.73. MdGA2ox8 contains conserved domains DIOX_N and 20G-Fell_Oxy, without obvious hydrophobic region, transmembrane domain and signal peptide. The MdGA2ox8 protein is a non-secreted protein. Phylogenetic analysis showed that the MdGA2ox8 protein was closely related to Pyrus bretschneideri GA2ox8 protein. The RT-qPCR results showed that MdGA2ox8 was differentially expressed in apple tissues, with the highest expression in flowers, followed by old leaf>young leaf>phloem, but almost no expression in xylem and fruitlet. Five transgenic positive lines were obtained by transferring MdGA2ox8 into model plant tobacco. Compared with the wild-type, the GA4 content decreased and GA1 content increased in overexpressed MdGA2ox8 tobacco plants. The average content of GA1 in wild-type and transgenic tobacco was 1.26 and 1.75 ng·g -1, respectively. The average content of GA4 in wild-type and transgenic tobacco was 5.43 and 1.07 ng·g -1, respectively. Compared with the wild-type, the total content of GA4 and GA1 in transgenic tobacco plants decreased, resulting in shorter internode length, dwarfing and delayed flowering. The average height of wild-type and transgenic tobacco plants was 38.50 and 7.36 cm, respectively. The average internode length of tobacco plants was 9.5 and 3.3 cm, respectively. The leaf of transgenic tobacco was dark green and the leaf aspect was reduced. Moreover, the expression level of endogenous gibberellin synthesis pathway-related genes NtGA3ox1 and NtGA3ox2 was positively regulated by MdGA2ox8 in transgenic tobacco.【Conclusion】The ORF of gibberellin 2-oxidase gene MdGA2ox8 was obtained. The difference of tissue expression in MdGA2ox8 was found. The total content of GA1 and GA4 in MdGA2ox8 overexpressed tobacco decreased, which resulted in the shortening of internode length and dwarfing of plants.

Key words: apple (Malus domestica), gibberellin oxidase, dwarf, MdGA2ox8, overexpression, tobacco

Table 1

Primers used for cloning, expression and functional analysis of MdGA2ox8"

引物名 Primer name 引物序列Primer sequence (5′-3′) 产物Product
MdGA2ox8-F1 ATGTCAATGAAGATTCTA 开放阅读框
ORF
MdGA2ox8-R1 TTATACGACAAATCTAGG
MdGA2ox8-F2 C/GAGCTC/ATGTCAATGAAGATTCTA 酶切位点SacⅠ/BamHⅠ
Restriction site SacⅠ/BamHⅠ
MdGA2ox8-R2 CG/GGATCC/TTATACGACAAATCTAGG
GA2ox8-RT-F GAGGAGTGCATGACGGAGAT 特异扩增
Special amplification
GA2ox8-RT-R ATGTGGCAGAAGGAGTTCCC
NtGA3ox1-RT-F ATCGACCTCGACAACTACATCA 特异扩增
Special amplification
NtGA3ox1-RT-R CAGGAGAACGAGCAGCCTTA
NtGA3ox2-RT-F GTAGTGAACCGAACCCGACA 特异扩增
Special amplification
NtGA3ox2-RT-R GCGCAAAGCTGAAAAGACGA
NtTubulin-F AGATGTTCCGTCGTGTCAGTG 烟草内参基因
Tubulin in tobacco
NtTubulin-R TGCTTCCTCTTCATCCTCATATCC
MdTubulin-F AGGATGCTACAGCCGATGAG 苹果内参基因
Tubulin in apple
MdTubulin-R GCCGAAGAACTGACGAGAATC

Fig. 1

Vector map of pCAMBIA1301-35SN-MdGA2ox8 (A) and agarose gel electrophoresis of MdGA2ox8 (B)"

Fig. 2

Alignment of amino acid sequences of MdGA2ox8 Red pane indicates the DIOX_N domain; black pane indicates the 2OG-FeII_Oxy domain"

Fig. 3

Phylogenetic tree of GA2ox8 from different plant species"

Fig. 4

Expression of MdGA2ox8 in different tissues of apple The different lowercases indicate significant difference (P<0.05). The same as below"

Fig. 5

GUS staining (A), PCR (B) and RT-PCR (C) amplification of transformation tobacco with MdGA2ox8"

Fig. 6

Determination of phenotype in transgenic tobacco plants"

Fig. 7

Effect of MdGA2ox8 overexpression on chlorophyll content in tobacco plants"

Fig. 8

Effect of MdGA2ox8 overexpression on gibberellin content in transgenic tobacco leaves"

Fig. 9

Detection of MdGA2ox8-overexpressed lines using RT-qPCR"

[1] 盛炳成, 渠慎春 . 苹果新品种‘苏帅’. 园艺学报, 2012,39(10):2075-2076.
SHENG B C, QU S C . A new apple cultivar ‘Sushuai’. Acta Horticulturae Sinica, 2012,39(10):2075-2076. (in Chinese)
[2] HEDDEN P, PHILLIPS A L . Gibberellin metabolism: new insights revealed by the genes. Trends in Plant Science, 2000,5(12):523-530.
doi: 10.1016/S1360-1385(00)01790-8 pmid: 11120474
[3] OLSZEWSKI N, SUN T, GUBLER F . Gibberellin signaling: biosynthesis, catabolism, and response pathways. The Plant Cell, 2002,14(Suppl. 1):S61-S80.
doi: 10.1105/tpc.010476
[4] DAYAN J, SCHWARZKOPF M, AVNI A, ALONI R . Enhancing plant growth and fiber production by silencing GA 2-oxidase. Plant Biotechnology Journal, 2010,8(4):425-435.
doi: 10.1111/j.1467-7652.2009.00480.x pmid: 20070875
[5] KOTODA N, MATSUO S, HONDA I, YANO K, SHIMIZU T . Gibberellin 2-oxidase genes from Satsuma mandarin (Citrus unshiu Marc.) caused late flowering and dwarfism in transgenic Arabidopsis. The Horticulture Journal, 2017,86(2):183-193.
[6] LEE D H, LEE I C, KIM K J, KIM D S, NA H J, LEE I J, KANG S M, JEON H W, LE P Y, KO J H . Expression of gibberellin 2-oxidase 4 from Arabidopsis under the control of a senescence-associated promoter results in a dominant semi-dwarf plant with normal flowering. Journal of Plant Biology, 2014,57(2):106-116.
[7] HUANG J, TANG D, SHEN Y, QIN B, HONG L, YOU A, LI M, WANG X, YU H, GU M, CHENG Z . Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.). Journal of Genetics and Genomics, 2010,37(1):23-36.
[8] 丁伟, 周葱, 刘超, 何家轩, 贾兵, 朱立武 . 缺铁胁迫对梨叶片中GA信号转导相关基因的影响. 西北植物学报, 2015,35(2):233-238.
doi: 10.7606/j.issn.1000-4025.2015.02.0233
DING W, ZHOU C, LIU C, HE J X, JIA B, ZHU L W . Effect of Iron-deficiency on the expression of GA signal transduction related genes in leaf ‘Dangshansuli’ pear (Pyrus bretschneideri Rehd.). Acta Botanica Boreali-Occidentalia Sinica, 2015,35(2):233-238. (in Chinese)
doi: 10.7606/j.issn.1000-4025.2015.02.0233
[9] 程飞飞 . 矮生梨‘中矮1号’ GA2-oxidase基因的克隆与功能分析[D]. 北京: 中国农业科学院, 2012.
CHENG F F . Cloning and function analysis of GA2-oxidase gene in dwarf pear of ‘Zhongai 1’[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012. ( in Chinese)
[10] 王西成, 任国慧, 房经贵, 李阿英, 刘洪, 吴伟民, 赵密珍 . 葡萄赤霉素合成相关基因克隆、亚细胞定位和表达分析. 中国农业科学, 2012,45(11):2224-2231.
doi: 10.3864/j.issn.0578-1752.2012.11.011
WANG X C, REN G H, FANG J G, LI A Y, LIU H, WU W M, ZHAO M Z . Cloning, subcellular localization and expression analysis of genes related to the synthesis of gibberellin from grapevine. Scientia Agricultura Sinica, 2012,45(11):2224-2231. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2012.11.011
[11] 石建斌 . 马铃薯赤霉素合成代谢途径关键酶基因GA2ox1、GA20ox1的克隆与功能分析[D]. 西宁: 青海大学, 2016.
SHI J B . The cloning and function analysis of key enzyme gene GA2ox1, GA20ox1 in gibberellin biosynthetic pathway of potato (Solanum tuberosum)[D]. Xining: Qinghai University, 2016. ( in Chinese)
[12] 孙同玉, 祝娟, 孙鹏, 廖登群, 李先恩, 祁建军 . 西洋参PqGA2ox基因的克隆与序列分析. 中草药, 2014,45(24):3599-3606.
doi: 10.7501/j.issn.0253-2670.2014.24.019
SUN T Y, ZHU J, SUN P, LIAO D Q, LI X E, QI J J . Cloning and sequence analysis of PqGA2ox gene in Panax quinquefolium. Chinese Traditional and Herbal Drugs, 2014,45(24):3599-3606. (in Chinese)
doi: 10.7501/j.issn.0253-2670.2014.24.019
[13] 丁铃, 江维克, 周涛, 李军, 郑伟, 龙登凯, 肖承鸿 . 太子参2个赤霉素2-氧化酶基因的克隆与序列分析. 中草药, 2017,48(15):3145-3152.
DING L, JIANG W K, ZHOU T, LI J, ZHENG W, LONG D K, XIAO C H . Molecular cloning and sequence analysis of two GA2ox genes in Pseudostellariae radix. Chinese Traditional and Herbal Drugs, 2017,48(15):3145-3152. (in Chinese)
[14] WUDDINEH W A, MAZAREI M, ZHANG J, POOVAIAH C R, MANN D G, ZIEBELL A, SYKES R W, DAVIS M F , STEWART JR C N. Identification and overexpression of gibberellin 2-oxidase (GA2ox) in switchgrass(Panicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance. Plant Biotechnology Journal, 2015,13(5):636-647.
[15] 肖政, 李纪元, 范正琪, 李辛雷, 殷恒福 . 荔波连蕊茶GA2ox1基因的克隆及表达分析. 林业科学研究, 2016,29(1):41-47.
XIAO Z, LI J Y, FAN Z Q, LI X L, YIN H F . Cloning and expression analysis of GA2ox1 gene from Camellia lipoensis. Forest Research, 2016,29(1):41-47. (in Chinese)
[16] YAN J, LIAO X, HE R, ZHONG M, FENG P, LI X, TANG D, LIU X, ZHAO X . Ectopic expression of GA 2-oxidase 6 from rapeseed (Brassica napus L.) causes dwarfism, late flowering and enhanced chlorophyll accumulation in Arabidopsis thaliana. Plant Physiology and Biochemistry, 2017,111:10-19.
doi: 10.1016/j.plaphy.2016.11.008
[17] ZHAO H, DONG J, WANG T . Function and expression analysis of gibberellin oxidases in apple. Plant Molecular Biology Reporter, 2010,28(2):231-238.
doi: 10.1007/s11105-009-0146-8
[18] KUSABA S, HONDA C, KANO-MURAKAMI Y . Isolation and expression analysis of gibberellin 20-oxidase homologous gene in apple. Journal of Experimental Botany, 2001,52(355):375-376.
doi: 10.1246/cl.2004.790 pmid: 11283183
[19] 白牡丹, 王彩虹, 田义轲, 陈宝印, 刘云龙 . 苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析. 华北农学报, 2012,27(5):55-59.
doi: 10.3969/j.issn.1000-7091.2012.05.011
BAI M D, WANG C H, TIAN Y K, CHEN B Y, LIU Y L . Gene structure identification and protein sequence bioinformation analysis for GA2ox from apical tissue of apple shoots. Acta Agriculturae Boreali-Sinica, 2012,27(5):55-59. (in Chinese)
doi: 10.3969/j.issn.1000-7091.2012.05.011
[20] 白牡丹, 王彩虹, 田义轲, 刘云龙, 陈宝印 . 柱型与普通型苹果茎尖组织中GA2ox基因的序列比较及表达量分析. 西北农业学报, 2012,21(11):124-127.
doi: 10.7606/j.issn.1004-1389.2012.11.023
BAI M D, WANG C H, TIAN Y K, LIU Y L, CHEN B Y . Sequence and expression analysis of GA2ox gene from shoots apical tissue between columnar and standard apple. Acta Agriculturae Boreali-occidentalis Sinica, 2012,21(11):124-127. (in Chinese)
doi: 10.7606/j.issn.1004-1389.2012.11.023
[21] 辛璐 . 短枝型苹果‘苏帅’赤霉素相关差异基因的筛选与表达分析[D]. 南京: 南京农业大学, 2015.
XIN L . Screening and expression of the difference for gibberellin related genes of spur-type apple ‘Sushuai’[D]. Nanjing: Nanjing Agricultural University, 2015. ( in Chinese)
[22] KUMAR S, STECHER G, TAMURA K . MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016,33(7):1870-1874.
doi: 10.1093/molbev/msw054
[23] HORSCH R B, FRY J E, HOFFMANN N L, EICHHOLTZ D, ROGERS S G, FRALEY R T . A simple and general method for transferring genes into plants. Science, 1985,227(4691):1229-1231.
doi: 10.1126/science.227.4691.1229 pmid: 17757866
[24] 赵勤 . 根癌农杆菌介导烟草K326遗传转化体系优化. 农业科学与技术, 2011,12(1):62-64.
doi: 10.3969/j.issn.0517-6611.2011.08.010
ZHAO Q . Optimization of genetic transformation system of tobacco K326 mediated by Agrobacterium. Agricultural Science and Technology, 2011,12(1):62-64. (in Chinese)
doi: 10.3969/j.issn.0517-6611.2011.08.010
[25] 李合生 . 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
LI H S. Physiological and Biochemical Experimental Principles and Techniques. Beijing: Higher Education Press, 2000. ( in Chinese)
[26] HEDDEN P, PHILLIPS A L . Manipulation of hormone biosynthetic genes in transgenic plants. Current Opinion in Biotechnology, 2000,11(2):130-137.
doi: 10.1016/S0958-1669(00)00071-9 pmid: 10753762
[27] THOMAS S G, PHILLIPS A L, HEDDEN P . Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation. Proceedings of the National Academy of Sciences of the United States of America, 1999,96(8):4698-4703.
doi: 10.1073/pnas.96.8.4698 pmid: 10200325
[28] SCHOMBURG F M, BIZZELL C M, LEE D J, ZEEVAART J A D, AMASINO R M . Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants. The Plant Cell, 2003,15(1):151-163.
doi: 10.1105/tpc.005975
[29] 王庆杰, 金仲鑫, 周李杰, 郝玉金, 姚玉新 . 苹果MdcyMDH过量表达对光合、激素和生长的影响. 中国农业科学, 2015,48(14):2868-2875.
doi: 10.3864/j.issn.0578-1752.2015.14.018
WANG Q J, JIN Z X, ZHOU L J, HAO Y J, YAO Y X . Impacts of MdcyMDH overexpression on photosynthesis, hormone and growth in apple. Scientia Agricultura Sinica, 2015,48(14):2868-2875. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2015.14.018
[30] 朱登峰 . 甘蓝型油菜中表达AtGA2ox8的表型分析及其对木质素生物合成的影响[D]. 海口: 海南大学, 2010.
ZHU D F . Effects of expressed AtGA20x8 on lignin biosynthesis and phenotypic analysis in transgenic Brassica napus plants[D]. Haikou: Hainan University, 2010. ( in Chinese)
[31] 刘露露, 鲁婷婷, 王爽, 李美靓, 赵双菁, 刘莹莹, 魏志刚 . 杨树PsnGA20ox1过表达对烟草叶片发育的影响. 北京林业大学学报, 2018,40(2):22-30.
LIU L L, LU T T, WANG S, LI M L, ZHAO S J, LIU Y Y, WEI Z G . Effects of poplar PsnGA20ox1 overexpression on leaf development of tobacco. Journal of Beijing Forestry University, 2018,40(2):22-30. (in Chinese)
[32] 李军, 赵爱春, DIANE U, 王茜龄, 刘长英, 鲁成, 余茂德 . 桑树MaDFR的克隆及功能分析. 中国农业科学, 2014,47(22):4524-4532.
LI J, ZHAO A C, DIANE U, WANG X L, LIU C Y, LU C, YU M D . Cloning and function analysis of a MaDFR gene from mulberry. Scientia Agricultura Sinica, 2014,47(22):4524-4532. (in Chinese)
[33] 李亚丽, 刘中来, 周洁, 徐国娟, 瞿绍洪 . 转mCherry基因水稻的遗传分析及T-DNA整合位点的研究. 分子植物育种, 2012,10(2):121-130.
doi: 10.3969/mpb.010.000121
LI Y L, LIU Z L, ZHOU J, XU G J, QU S H . Genetic analysis of mCherry transgenic rice and molecular characterization of T-DNA integration sites in the rice genome. Molecular Plant Breeding, 2012,10(2):121-130. (in Chinese)
doi: 10.3969/mpb.010.000121
[34] XIAO Z, FU R, LI J, FAN Z, YIN H . Overexpression of the gibberellin 2-oxidase gene from Camellia lipoensis induces dwarfism and smaller flowers in Nicotiana tabacum. Plant Molecular Biology Reporter, 2016,34(1):182-191.
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