Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (16): 3155-3163.doi: 10.3864/j.issn.0578-1752.2017.16.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Response of Anammox Bacteria Community Structure and Vertical Distribution to Different Long-Term Fertilizations in Calcareous Purple Paddy Soil

WANG YingYan1, LU ShengE1, LI YueFei1, TU ShiHua2, ZHANG XiaoPing1, GU YunFu1   

  1. 1Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 611130; 2Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066
  • Received:2017-01-03 Online:2017-08-16 Published:2017-08-16

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Abstract: 【Objective】The impacts of long-term fertilizations on the anaerobic ammonium oxidation bacteria (AAOB) community structure and vertical distribution were studied in order to deeply understand the microbial mediated mechanism of anammox reaction, develop a sound fertilization regime and provide theoretical foundations for maintaining the soil quality in this region.【Method】Chemical analysis, real-time PCR and terminal restriction fragment length polymorphism (T-RFLP) were used to analyze the soil physico-chemical properties, the 16S rRNA gene abundance and composition of AAOB community, respectively.【Result】The results showed that compared to no fertilizer application control (CK), all fertilizers decreased the soil pH and nitrate content, while increased soil organic matter (SOM), total nitrogen (TN) and ammonia content. With increasing of soil depth, soil parameters including soil pH, TN, and nitrate content were decreased, while the ammonia content was not obviously changed. The qPCR results showed: the abundance of AAOB 16S rRNA gene peaked in the 0-20 cm layer, while being the lowest in the 20-40 cm depth. Mere nitrogen treatment (N) had an evident positive effect on the abundance of AAOB. The T-RFLP results indicated that in the 0-20 cm layer, the community compositions of AAOB were the most complex, which were also supported by the highest Shannon-wiener diversity index. Compositions of AAOB community in the soil fertilized with N only were the simplest, while those in the soil amended with CK were the richest. Besides, the predominant AAOB were phylogenetically affiliated to Candidatus Brocadia. Redundant gradient analysis (RDA) showed that pH was the key factor in shaping the AAOB community in calcareous purple paddy soil amended with different fertilizer treatments.【Conclusion】This study suggested that mere N fertilizer would decrease the compositions of AAOB community in calcareous purple paddy soil but increase their abundances. The topsoil (0-20 cm) was the main distribution soil depth in calcareous purple paddy soil for AAOB.

Key words: long-term fertilization, Calcareous Purple Paddy soil, T-RFLP, anammox bacteria

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