Abstract: 【Objective】The objective of this experiment is to improve and optimize the ABS vassal casting technique, establish a casting method for a Stereoscopic specimen of pig bronchial arteries, cast a stereoscopic specimens of pig bronchial arteries, thus providing an experimental way for the study of the branching structure and distribution of pig bronchial arteries in lung.【Method】With independent and combined casting methods of bronchial arteries, independent and combined bronchial artery casting specimens were obtained through improving and optimizing the ABS casting method. 【Result】 A method was established for making casts of the stereoscopic structure of bronchia arteries of a pig. This study proposed the steps and methods for making casts of the porcine independent and combined bronchial arteries. The steps and methods for making independent casts of the bronchial arteries were as follows: Firstly, the thoracic aorta was ligated and intubated in the proximal and distal ends of the thoracic aorta. Meanwhile the front end and the rear end of the esophagus were ligated at the corresponding position of the thoracic aorta intubation and the trachea was inserted the blind end sleeve and ligated in order to prevent the leakage around the trachea and esophagus vascular network during the infusion. Secondly, 60 mL of 10% ABS and 100-160 mL of 15% ABS were forced into the aorta through the aorta intubations indirectly. Infusion should be stopped when the middle part of rubber tube dilated. When the dilated part becomes smaller, 20% ABS should be infused to ensure the normal pressure after the end of the continuous infusion 5 h later. Thirdly, these specimens were hardened in water for 3 to 4 d, and then corroded with hydrochloric acid for about 10 d. At last, the integral specimens were obtained though washing under the running water and high pressure, and trimming elaborately. The major points for making combined casting of bronchial artery and bronchial tree were as follows: Firstly, the thoracic aorta, esophagus and trachea were simultaneously intubated and ligated. Secondly, the bronchial artery was infused with indirect aorta intubations. At last the combined casting of bronchial artery and bronchial tree was obtained through the process of hardening, corrosion, washing and repairing. The major points for making combined casting of bronchial artery and pulmonary artery were as follows: The method for making combined casting of bronchial artery and bronchial tree is similar to the method of making combined casting of bronchial artery and pulmonary artery. However, the former and the latter have the differences. For the former, the casting agent is infused into bronchial tree, while pulmonary artery for the latter. The integral independent bronchial artery cast specimens, the combined cast specimens of bronchial artery and bronchial tree and the combined cast specimens of bronchial artery and pulmonary artery were obtained in this study.【Conclusion】The three-dimensional structure of these cast specimens was clear, which could completely display the origin, course, branching and distribution. Meanwhile this model could progressively display the relationships between the bronchial artery and bronchial tree or pulmonary artery. The establishment of the methods could make a good foundation for the study of bronchial artery of pig and other animals.

Key words: pig , bronchial artery , specimen , casting method