Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1939-1945.doi: 10.3864/j.issn.0578-1752.2013.09.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Expression of Ghrelin and GHS-R1a mRNA in the Bovine Oocytes During in Vitro Maturation

 MI  Yan, ZHANG  Ling-Li, LI  Hai-Jun, DU  Chen-Guang, CAO  Gui-Fang   

  1. 1.College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018
    2.Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109, Inner Mongolia
  • Received:2012-12-04 Online:2013-05-01 Published:2013-03-18

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Abstract: 【Objective】 The aim of this study was to investigate the possibility of Ghrelin and GHS-R1a mRNA expression in bovine oocytes produced in vitro and the relationship between FSH and the expression of Ghrelin and GHS-R1a mRNA. 【Method】Relative real time RT-PCR technology was applied to detect the pattern of Ghrelin and GHS-R1a mRNA expression in oocytes, and the effect of different concentrations FSH and the combination of FSH and FSH binding inhibitor on the expression of Ghrelin and GHS-R1a mRNA were observed. 【Result】 Relative real time RT-PCR experiments confirmed that the Ghrelin and GHS-R1a mRNA levels in oocyte varied depending on the developmental stage, with the significant decrease of Ghrelin and GHS-R1a mRNA expression at 8 h in oocyte maturation process. And addition of FSH inhibited the expression of Ghrelin and GHS-R1a mRNA within oocytes, and this inhibition effect could be attenuated by FSH binding inhibitor. 【Conclusion】 The expression of Ghrelin and GHS-R1a mRNA could be declined with the rising of FSH concentration during in vitro maturation of bovine oocytes.

Key words: bovine , oocytes , in vitro maturation , FSH , Ghrelin , GHS-R1a

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