Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1668-1675.doi: 10.3864/j.issn.0578-1752.2013.08.016

• HORTICULTURE • Previous Articles     Next Articles

Fluoride Accumulation and Its Subcellular Distribution in Three Tea Plants

 CAI  Hui-Mei, PENG  Chuan-Yi, LI  Cheng-Lin, GAO  Zhu, HOU  Ru-Yan, WAN  Xiao-Chun   

  1. 1.College of Tea&Food Science and Technology, Anhui Agricultural University/Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education & ministry of Agriculture, Anhui Agricultural University, Hefei 230036
    2.Anhui Lükui tea industry Co.,   Ltd, Xuancheng,242131, Anhui
    3.China National Center for Quality Supervision and Test of Agricultural-Avocation        Processed Food, Hefei 230001
  • Received:2012-08-17 Online:2013-04-15 Published:2013-01-23

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Abstract: 【Objective】The objective of this study was to give some experimental basis for studying the absorption, accumulation of F and the mechanism of detoxification.【Method】Solution culture and differential centrifugation were conducted to study the characteristics of F accumulation and its subcellular distribution of Pingyangtezao, Wuniuzao and Fuzao No.2, with comparison of the differences between varieties.【Result】F concentration in roots, stems and leaves of three kinds of tea plants increased with F treatment, under 0-10 mg•L-1 F treatments, the ground part of fluoride content was greater than the root(Translation correlation ＞1) and 56% of F was distributed in above-ground part. Except for the control, most of the fluoride was distributed in cell wall (76.84%-91.58%) and soluble fraction (53.24%-80.35%) of leaf and root. F treatment generally increased the percentage of cell wall and soluble fraction, but decreased that of cell organelle. In F treatment, the change tendency of F concentration of subcellular fractions of leaf of Pingyangtezao and Wuniuzao ranked in the following order: cell wall＞ soluble fraction＞ cell organelle, while soluble fraction＞cell wall＞ cell organelle for Fuzao No.2 . In low F (0-5 mg•L-1 ) treatment , the change tendency of F concentration of subcellular fractions of root of three kinds of tea plants ranked in the following order: cell organelle≥cell wall＞soluble fraction, while cell wall＞soluble fraction≥cell organelle in high F (10-50 mg•L-1 ) treatment.【Conclusion】Translation correlation , percentage of F for above-ground part and F subcellular distribution in root and leaf suggested tea plant was of strong upward transportation capacity. The subcellular distribution of F in leaves and roots were related with tea species and solution F treatment, and so did the correlation between the concentration of T-F and subcellular fractions in leaf, with some differences, but no in root. Cell wall and soluble fraction were the main storage sites in high F treatment, suggesting cell wall binding and vacuolar compartmentalization may be main reasons for tea plant to detoxify fluoride toxicity.

Key words: tea varieties , fluoride , accumulation , subcellular distribution

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