Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (6): 1208-1212.doi: 10.3864/j.issn.0578-1752.2013.06.014

• HORTICULTURE • Previous Articles     Next Articles

Evaluation on Resistance of Grape Gray Mold Pathogen Botrytis cinerea to Pyrimethanil in China

 ZHANG  Wei, QIAO  Guang-Xing, HUANG  Jin-Bao, WANG  Zhong-Yue, LI  Xing-Hong   

  1. 1.Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097
    2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2012-12-11 Online:2013-03-15 Published:2013-01-25

PDF

967

Cited

21

Abstract: 【Objective】The resistance to pyrimethanil of Botrytis cinerea isolated from diseased grape berries in China was studied.【Method】A total of 104 single conidial isolates of B. cinerea were isolated from diseased grape berries which were collected from vineyards located in 14 different cities or suburban counties in China. The method of mycelial growth inhibition was used to determine the resistance of B. cinerea to anilinopyrimidine fungicides pyrimethanil.【Result】The findings in the current work demonstrated that the resistance frequency of B. cinerea to pyrimethanil was 22.22% to 62.5%. Most of the resistant isolates showed middle resistant or high resistant phenotype, and the resistant frequency of high resistant isolates reached up to 44.23%. Resistance to pyrimethanil of B. cinerea differs in different viticulture climate zones. 【Conclusion】Resistance to pyrimethanil of B. cinerea isolated from grape diseased samples in China is commonly present, and the resistant isolates showed cross resistance. Accordingly, the frequency of use of pyrimethanil should be limited in the prevention of grape gray mold, and fungicides such as dicarboximide and carbamates can be used alternatively. It was suggested that novel fungicides or biofungicides should be introduced for the control of grape gray mold.

Key words: grape , Botrytis cinerea , pyrimethanil , resistance

[1]丁中, 刘峰, 王会利, 吴春先, 慕立义. 番茄灰霉菌的多重抗药性研究. 山东农业大学学报: 自然科学版, 2001, 32(4): 452-456.

Ding Z, Liu F, Wang H L, Wu C X, Mu L Y. Study on multiple fungicide resistance to Botrytis cinerea. Journal of Shandong Agricultural University: Nature Science, 2001, 32(4): 452-456. (in Chinese)

[2]Bouchra C, Achouri M, Idrissi Hassani L M, Hmamouchi M. Chemical composition and antifungal activity of essential oils of seven Moroccan Labiatae against Botrytis cinerea Pers: Fr.. Journal of Ethnopharmacology, 2003, 89(1): 165-169.

[3]Elad Y, Yunis H, Katan T. Multiple fungicide resistance to benzimidazole, dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel. Plant Pathology, 1992, 41(1): 41-46.

[4]Raposo R, Delcan J, Gomez V, Melgarejo P. Distribution and fitness of isolates of Botrytis cinerea with multiple fungicide resistance in Spanish greenhouses. Plant Pathology, 1996, 45(3): 497-505.

[5]Kim B S, Park E W, Cho K Y. Population dynamics of sensitive and resistance phenotypes of Botrytis cinerea to benzimidazole, dicarboximide and N-phenylearbamate fungicides in Korea．Journal of Pesticide Science, 2000, 25(4):385-386.

[6]严红, 燕继晔, 王忠跃, 李亚宁, 金桂华, 李兴红. 葡萄灰霉病  菌对3种杀菌剂的多重抗药性检测. 果树学报, 2012, 29(4): 625-629.

Yan H, Yan J Y, Wang Z Y, Li Y N, Jin G H, Li X H. Multiple fungicide resistance of Botrytis cinerea from grapevine to three fungicides. Journal of Fruit Science, 2012, 29(4): 625-629. (in Chinese)

[7]朱桂宁, 黄福新, 蔡健和, 秦碧霞, 刘志明. 广西番茄灰霉病菌的多重抗药性检测. 中国蔬菜, 2003(4): 14-16.

Zhu G N, Huang F X, Cai J H, Qin B X, Liu Z M. Study on multiple fungicide resistance in Botrytis cinerea from tomato in Guangxi. China Vegetables, 2003(4): 14-16. (in Chinese)

[8]乔广行, 严红, 金桂华, 李兴红. 北京地区番茄灰霉病菌的多重抗药性检测. 植物保护, 2011(5): 176-180.

Qiao G H, Yan H, Jin G H, Li X H. Detection of multiple fungicide resistance in Botrytis cinerea from tomato in Beijing. Plant Protection, 2011(5): 176-180. (in Chinese)

[9]Northover J, Matteon J A. Resistance of Botrytis cinerea to benomyl and iprodione in vineyards and greenhouses after exposure to the fungicides alone or mixed with captan. Plant Disease, 1986, 70: 398-402.

[10]Latorre B, Flores V, Sara A, Roco A. Dicarboximide-resistant strains of Botrytis cinerea from table grapes in Chile: Survey and characterization. Plant Disease, 1994, 78(10): 990-994.

[11]Esterio M, Muñoz G, Ramos C, Cofré G, Estévez R, Salinas A, Auger J. Characterization of Botrytis cinerea isolates present in Thompson seedless table grapes in the Central Valley of Chile. Plant Disease, 2011, 95(6): 683-690.

[12]Rosslenbroich H J, Stuebler D. Botrytis cinerea history of chemical control and novel fungicides for its management. Crop Protection, 2000, 19: 557-561.

[13]Heye U J, Speicb J, Siegle H, Steinemann A, Forster B, Knauf-Beiter G, Herzog J, Hubele A. CGA 219417: a novel broad-spectrum fungicide. Crop Protection, 1994, 13(7): 541-549.

[14]Leroux, P. Recent development in the mode of action of fungicides. Pesticides Science, 1996, 47(2): 191-197.

[15]Hilber U W, Hilber-Bodmer M. Genetic basis and monitoring of resistance of Botryotinia fuckeliana to anilinopyrimidines. Plant Disease, 1998, 82(5): 496-500.

[16]Leroux P, Chapeland F, Desbrosses D, Gredt M. Patterns of cross-resistance to fungicides in Botryotinia fuckeliana (Botrytis cinerea) isolates from French vineyards. Crop Protection, 1999, 18(10): 687-697.

[17]Latorre B A, Spadaro I, Rioja M E. Occurrence of resistant strains of Botrytis cinerea to anilinopyrimidine fungicides in table grapes in Chile. Crop Protection, 2002, 21(10): 957-961.

[18]纪明山, 祁之秋, 王英姿, 程根武，谷祖敏. 番茄灰霉病菌对嘧霉胺的抗药性. 植物保护学报, 2003, 30(4): 396-400.

Ji M S, Qi Z Q, Wang Y Z, Cheng G W, Gu Z M. Resistance of Botrytis cinerea to pyrimethanil in tomato. Acta Phytophylacica Sinica, 2003, 30(4): 396-400. (in Chinese)

[19]贾晓华. 番茄灰霉病菌和油菜菌核病菌对嘧霉胺的敏感性基线及番茄灰霉病菌抗药性研究[D]. 南京: 南京农业大学, 2004.

Jia X H. [D]. Sensitivity Baselines of Botrytis cinerea and Sclerotinia sclerotiorum to Pyrimethanil and Resistance of Botrytis cinerea[D]. Nanjing: Nanjing Agricultural University, 2004. (in Chinese)

[20]印利梅, 礼茜, 李红叶. 浙江省茄科蔬菜灰霉病菌对嘧霉胺的抗性. 浙江农业学报, 2007, 19(2): 123-126.

Yin L M, Li Q, Li H Y. Resistance of Botrytis cinerea to fungicide pyrimethanil from Solanaceae vegetables in Zhejiang Province. Acta Agriculturae Zhejiangensis, 2007, 19(2): 123-126. (in Chinese)

[21]赵晓军, 任璐, 周建波, 殷辉. 山西省蔬菜灰霉病菌对嘧霉胺的抗药性检测. 植物保护, 2012, 38(5): 120-123.

Zhao X J, Ren L, Zhou J B, Yin H. Resistance of Botrytis cinerea to pryimethanil on vegetables in Shanxi Province. Plant Protection, 2012, 38(5): 120-123. (in Chinese)

[22]李兴红, 乔广行, 黄金宝, 林雪, 刘建华. 北京地区番茄灰霉病菌对嘧霉胺的抗药性检测. 植物保护, 2012, 38(4): 141-143.

Li X H, Qiao G H, Huang J B, Lin X, Liu J H. Detection of the resistance of Botrytis cinerea to pyrimethanil from tomato in Beijing. Plant Protection, 2012, 38(4): 141-143. (in Chinese)

[23]贺普超. 葡萄学. 北京: 中国农业出版社, 1994.

He P C. Grapevine. Beijing: China Agriculture Press, 1994. (in Chinese)

[24]赵琳, 纪明山, 祁知秋, 程根武. 辽宁省蔬菜灰霉病菌对嘧霉胺的抗药性. 植物保护, 2008, 34(3): 85-88.

Zhao L, Ji M S, Qi Z Q, Cheng G W. Study on resistance of Botrytis cinerea to pyrimethanil in vegetable of Liaoning Province. Plant Protection, 2008, 34(3): 85-88. (in Chinese)
[1] ZHANG DongMei, ZHOU XinXin, XIAO GuiLin, ZENG XiangGuo, WANG ChunYan, WANG ZeXian, HAN YongChao. Phenotypic Characteristics of Strawberry Floral Organs in Response to Botrytis cinerea Infection and Methods for Gray Mold Resistance Evaluation [J]. Scientia Agricultura Sinica, 2026, 59(7): 1456-1466.
[2] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[3] LIU HaiQing, JIN JiaoJiao, SUN WanCang, CHAI Peng, QI WeiLiang, YANG Gang, LI Chan, LUO XueMei, SU YunYun, QIN XueXue. Morphogenesis of the Low-Growth Point and Its Multi-Hormonal Regulatory Mechanism During Overwintering in Winter Rapeseed (Brassica napus L.) [J]. Scientia Agricultura Sinica, 2026, 59(5): 951-966.
[4] CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[5] FENG WeiQing, NI YuanQian, FEI Teng, LI YouMei, XIE ZhaoSen. Differences in Vascular Bundle Morphological Structure, Distribution, and Water Transport Function in Grape Fruits of Different Shapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 161-178.
[6] WANG SiQi, ZOU LiRen, BAI RuiWen, YAN Ke, WANG SiYang, QI XiaoGuang, SHEN HaiLin, WEN JingHui. Screening of Key Genes Related to Gibberellic Acid Regulation of Rachis Hardening in Honey Grapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 179-189.
[7] TAN XiBei, LAN XuYing, LIU ChongHuai, FAN XiuCai, JIANG JianFu, SUN Lei, LI Peng, YU ShuXin, ZHANG Ying. Changes of Secondary Metabolites in Grapes with Different Resistance Levels in Response to White Rot Infection [J]. Scientia Agricultura Sinica, 2025, 58(9): 1767-1778.
[8] TANG XueShen, DANG ShiZhuo, ZHOU Juan, LI JiaHao, LI MeiHua, HU Hao, ZHANG YaHong. Analysis of VvBES1-1 Involvement in Flower Bud Differentiation of Red Globe Grape Based on Red and Blue Light Regulation [J]. Scientia Agricultura Sinica, 2025, 58(8): 1650-1662.
[9] YANG CaiLi, LI YongZhou, HE LiangLiang, SONG YinHua, ZHANG Peng, LIU ZhaoXian, LI PengHui, LIU SanJun. Genome-Wide Identification and Analysis of TPS Gene Family and Functional Verification of VvTPS4 in the Formation of Monoterpenes in Grape [J]. Scientia Agricultura Sinica, 2025, 58(7): 1397-1417.
[10] YUE RunQing, LI WenLan, DING ZhaoHua, MENG ZhaoDong. Molecular Characteristics and Resistance Evaluation of Transgenic Maize LD05 with Stacked Insect and Herbicide Resistance Traits [J]. Scientia Agricultura Sinica, 2025, 58(7): 1269-1283.
[11] ZOU XiaoWei, XIA Lei, ZHU XiaoMin, SUN Hui, ZHOU Qi, QI Ji, ZHANG YaFeng, ZHENG Yan, JIANG ZhaoYuan. Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2025, 58(6): 1116-1130.
[12] ZHANG TianYu, LI Bai, ZANG JinPing, CAO HongZhe, DONG JinGao, XING JiHong, ZHANG Kang. Genome-Wide Identification and Expression Analysis of HMG Family Genes in Botrytis cinerea [J]. Scientia Agricultura Sinica, 2025, 58(4): 704-718.
[13] GUO AoLin, LIN JunXuan, LAI GongTi, HE LiYuan, CHE JianMei, PAN Ruo, YANG FangXue, HUANG YuJi, CHEN GuiXin, LAI ChengChun. Effect of VdF3′5′H2 Overexpression on the Accumulation of Anthocyanin Composition in Spine Grape Cells [J]. Scientia Agricultura Sinica, 2025, 58(4): 802-818.
[14] ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.
[15] QIN Lu, SHEN DanDan, JIANG XiaoLi, XIE HePing, AO YiJun, YANG Yang, ZHU Feng, XU RangWei, LIAO WenYue, CHENG YunJiang. Effect of Water Status on the Storability of Citrus Fruits Harvested Under Continuous Rainy Weather [J]. Scientia Agricultura Sinica, 2025, 58(24): 5259-5273.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd