Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2345-2352.doi: 10.3864/j.issn.0578-1752.2013.11.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Optimization of Reaction Conditions for Cello-Oligosaccharides Production from Wheat Straw by β-Glucanase Hydrolysis

 LIU  Sha-Sha, LI  Jing-Mei, SHI  Bo, LIANG  Ping, LI  Chao   

  1. 1.Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081
    2.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijng 100081
    3.Food Science and Nutritional Engineering College, China Agricultural University,Beijing 100083
  • Received:2012-10-15 Online:2013-06-01 Published:2013-03-06

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Abstract: 【Objective】 The aim of this study was to explore the optimum conditions of wheat straw degradation by β-glucanase.【Method】The mixed-acid hydrolysis and chromatography were selected for the separation process, in which the cello- oligosaccharides with degrees of polymerization from 2 to 5 were obtained. The single factor experiments for optimal conditions were temperature, pH, E/S concentration of wheat straw and the hydrolysis time. The optimal conditions for hydrolysis of wheat straw were obtained through analyzing the yield of cellobiose and the conversion rate of wheat straw.【Result】The experimental results showed that by activated carbon column chromatography, the standard cello-oligosaccharides were obtained and the optimum conditions for wheat straw hydrolysis were as follows: the reaction temperature was 50℃, pH was 5.5, E/S was 0.4, concentration of wheat straw was 2%, and hydrolysis time was 10 h. Using the optimum conditions, the cellobiose yield was 148.15 mg•g-1 wheat straw and the conversion rate of wheat straw was 55.57%. 【Conclusion】 The yield of cellobiose and the conversion rate of wheat straw were both increased under the optimum conditions. It was found that the chromatographic columns composed of charcoal could separate well cello-oligosaccharides with degrees of polymerization from 2 to 5.

Key words: wheat straw , cellobiose , percent conversion , hydrolysis

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