宰后鸭肉骨骼肌中caspase-3活化对嫩度的影响

doi:10.3864/j.issn.0578-1752.2012.07.015

Abstract

Abstract: 【Objective】The objective of the experiment is to investigate whether the caspase-3 is activated postmortem and involved in the hydrolysis of the cytoskeleton process in duck, provide new experimental evidence to explain the postmortem conditioning of waterfowl by the mechanism of apoptosis.【Method】The cytoplasm of cytochrome-C levels, active caspase-3, -8 and -9 and cleaved PARP fragment expression of duck breast and thigh meat after slaughter were detected by immunochemical analysis, changes of caspase-3,-8 and -9 activity and the changes of shear force value were also evaluated.【Result】Results showed that cytochrome-c levels in cytosol were increased significantly (P＜0.05), caspase-3, -8 and -9 were split into 17 kD,18 kD and 23 kD fragments and there were higher activities at postmortem respectively (P＜0.05). PARP as a marker protein of caspase enzyme system was split into small fragments. Shear force values of breast and thigh meat were also significantly decreased at 4-24 h after slaughter (P＜0.05). Correlation analysis showed that caspase-8 and -9 activities were positively correlated to the reported caspase-3 activities and the shear force of muscles were negatively correlated with the activities of caspase-3 after slaughter in muscles (P＜0.01).【Conclusion】The results of procaspase-3 was significantly activated and the activities were increased which indicated that the postmortem activation of caspase-3 appears to be involved in both caspase-8 and caspase-9 pathway. Cleaved PARP and shear force values were reduced which indicated that caspase protease might have direct or indirect effects on maturation process during postmortem conditioning.

Key words: caspase-3, caspase activity, shear force values, duck, postmortem conditioning

HUANG Wei, CAO Jin-Xuan, WANG Dao-Ying, XU Wei-Min, ZHANG Mu-Han. Impact of Caspase-3 Activation on the Tenderness of Duck Skeletal Muscle During Postmortem Conditioning[J].Scientia Agricultura Sinica, 2012, 45(7): 1372-1379.

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Impact of Caspase-3 Activation on the Tenderness of Duck Skeletal Muscle During Postmortem Conditioning

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