Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1150-1158.doi: 10.3864/j.issn.0578-1752.2012.06.013

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Free and Bound Phenolic Contents and Antioxidant Activity of Five Varieties of Indica Rice Husk

 LI  Qing, ZHANG  Ming-Wei, ZHANG  Rui-Fen, WEI  Zhen-Cheng, DENG  Yuan-Yuan, TANG  Xiao-Jun, ZHANG  Ye-Hui, LI  Wu, MA  Yong-Xuan   

  1. 1.华中农业大学食品科技学院，武汉 430070
    2.广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室，广州 510610
  • Received:2011-10-18 Online:2012-03-15 Published:2011-12-15

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Abstract: 【Objective】 The contents and compositions of free and bound phenolics and antioxidant activity of husks of different rice varieties were compared. 【Method】 The contents of free and bound phenolics and flavonoids of rice husks of five different varieties were determined. High performance liquid chromatography (HPLC) method was used to measure the compositions and contents of phenolics in rice husks. The antioxidant activity was analyzed using the ferric reducing ability of plasma (FRAP) and ABTS radical cation scavenging activity assays. 【Result】 The free , bound and total phenolic contents of the husks of 5 tested indica rice varieties ranged from 42.8 to 123.0, 260.9 to 325.2 and 320.2 to 398.3 mg of gallic acid equivalents/100 g dry weight (DW), respectively. The average percentage contribution of bound phenolics to the total was 78.9%. The free, bound and total flavonoids contents of the husks ranged from 34.0 to 58.0, 47.9 to 64.4 and 82.0 to 115.7 mg of catechin equivalents/100 g DW, respectively. The average percentage contribution of bound flavonoids to the total was 56.4%. Gallic acid, protocatechuic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid and ferulic acid were detected in phenolic extracts of rice husks. Syringic acid and p-coumaric acid were the major free phenolics, with average of 25.09 μg•g-1 DW and 31.21 μg•g-1 DW, respectively. The most abundant bound phenolics was p-coumaric acid, and its average content was 2141.61 μg•g-1 DW. The free , bound and total FRAP antioxidant activity of the husks ranged from 37.7 to 106.0, 217.9 to 281.0, and 269.3 to 370.3 mg Trolox equivalents/100 g DW. The average percentage contribution of bound FRAP antioxidant activity to the total was 77.3%. The free , bound and total ABTS antioxidant activities of the husks ranged from 26.3 to 85.8, 67.2 to 111.9, and 93.4 to155.2 mg Trolox equivalents/100 g DW. The average percentage contribution of bound ABTS antioxidant activity to the total was 64.8%.【Conclusion】 Rice husk is rich in phenolics and possesses high antioxidant activity. Total phenolics and flavonoids, individual phenolics and antioxidant activity of 5 the tested rice husks were mainly contributed in bound form. Significant varietal differences were found. It is indicated that rice husk can be considered as a potential valuable source of the natural antioxidants.

Key words: rice variety, husk, phenolics, antioxidant activity

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