Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1144-1149.doi: 10.3864/j.issn.0578-1752.2012.06.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of Exogenous Divalent Cations Ca2+, Mg2+, and Cu2+ and Acriflavine on Ascorbate Biosynthesis in Rosa roxburghii Fruits

 ZHANG  Xue, YANG  Man, AN  Hua-Ming, HUANG  Wei, LIU  Wei   

  1. 贵州大学农学院/贵州省果树工程技术研究中心，贵阳 550025
  • Received:2011-09-22 Online:2012-03-15 Published:2011-11-24

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Abstract: 【Objective】 The effects of divalent cations and organic inhibitors on GalLDH activity and AsA biosynthesis in Rosa roxburghii fruits were studied.【Method】Fruits of Rosa roxburghii Tratt cv. Guinong 5 was used to investigate the effects of divalent cations Ca2+, Mg2+, and Cu2+ and organic inhibitors acriflavine and lycorine on GalLDH activity and AsA biosynthesis, by using the methods of incubation in vitro and injection in vivo.【Result】The results showed that the activity of purified GalLDH was promoted by Ca2+ and Mg2+, but extremely inhibited by Cu2+. Even so, when flesh tissues of the fruit was incubated respectively in the mixed solution of the metal ions together with L-galactono-1,4-lactone (GalL), an immediate substrate of AsA biosynthesis, both Ca2+ and Mg2+ could not effectively improve the AsA biosynthesis, while the AsA content in the flesh tissues pre-incubated in presence of Cu2+ decreased markedly. AsA biosynthesis and accumulation in developing fruit was obviously promoted by injection of Ca2+ solution and inhibited by that of Cu2+, but not influenced by Mg2+. Acriflavine, suggested an organic inhibitor of AsA biosynthesis, was able to inhibit the activity of Rosa roxburghii GalLDH about 70%, but lycorine, another specific inhibitor of AsA biosynthesis, had a very slight influence on it. The similar inhibitory effect was detected in incubating fresh fruit tissues in the presence of the two inhibitors together with GalL respectively, although injection of acriflavine presented a visible inhibiting effect on AsA accumulation in developing fruit, and lycorine had no influence.【Conclusion】Exogenous Ca2+ could promote but Cu2+ or acriflavine inhibit AsA biosynthesis in Rosa roxburghii fruits.

Key words: Rosa roxburghii Trattl divalent cations, aciflavine, lycorine, GalLDH, ascorbate

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