米糠酚类物质的大孔树脂分离纯化工艺

doi:10.3864/j.issn.0578-1752.2016.19.013

Abstract

Abstract: 【Objective】The objective of this study is to screen a resin with a good adsorption and desorption performance to rice bran polyphenols (RBP) and optimize the purification technique, to compare the total phenols, total flavonoids content and antioxidant activity before and after purification, thus providing a theoretical basis for the deep processing and utilization of rice bran.【Method】The behavior of static adsorption and desorption, dynamic adsorption and desorption of 9 macroporous resins (HPD-100, HPD-200A, HPD-300, HPD-700, D101, HPD-722, AB-8, ADS17, and HPD-826) with different polarities to RBP was determined. Kinetic characteristics of static adsorption of the preferred resin were analyzed. The purification technique of macroporous resin to RBP was developed through the dynamic adsorption and desorption curves under different loading and elution conditions, including phenolic concentrations in loading samples, sampling rates, sampling volumes and ethanol concentrations in eluent. The phenolic profiles in RBP before and after resin purification were analyzed by HPLC analysis. Their total phenolic and flavonoid contents, oxygen radical absorption capacity (ORAC) and cellular antioxidant activity (CAA) were also estimated. 【Result】HPD-300 exhibited the best capability of adsorption and desorption to RBP among the 9 selected macroporous resins. The saturated absorption quantity of HPD-300 resin to RBP was 9.04 mg GAE·g^-1, and the desorption rate was 82.62%. It took 6 h for HPD-300 resin to reach absorption equilibrium. The optimized purification parameters were as follows: the phenolic concentration of loading sample is 1 mg GAE·mL^-1, sampling rate is 3.0 BV·h^-1, sampling volume is 3.5 BV, eluent is 70% ethanol, flow velocity is 3.0 B V·h^-1. After HPD-300 resin purification, the total phenolic content increased by 2.6 times from 88.07 µg GAE·mg^-1 to 320.72 µg GAE·mg^-1, and the total flavonoids content increased by 3.4 times from 30.04 µg CE·mg^-1 to 133.67 µg CE·mg^-1. Ten phenolic compounds were identified from rice bran phenolic extract, which were gallic acid, protocatechuic acid, vanillic acid, p-hydroxy benzoic acid, caffeic acid, clove acid, epicatechin, catechins, vanillic aldehyde, p-coumaric acid and ferulic acid by HPLC analysis. The phenolic profiles of the extract were not changed after HPD-300 resin purification, but the concentrations of 10 phenolic compounds increased by 0.4-2.4 times. The ORAC and CAA antioxidant activity of the extract changed from 1 327.51 µmol TE·g^-1 and 29.19 µmol QE·g^-1 before purification to 3 248.21 µmol TE·g^-1 and 95.24 µmol QE·g^-1 after purification, and increased by 1.4 and 2.2 times, respectively.【Conclusion】The total phenolic and flavonoid contents and antioxidant activity increased by 1-3 times after HPD-300 resin purification. The individual phenolic compounds in the extract were not lost significantly during purification. Therefore, HPD-300 macroporous resin was suitable to purify rice bran phenolic extract.

Key words: rice bran, phenolics, macroporous resin, purification, CAA, ORAC

TONG Xin, ZHANG Rui-fen, DENG Yuan-yuan, XIAO Juan, LIU Lei, ZHANG Yan, WEI Zhen-cheng, ZHANG Ming-wei . Separation and Purification of Polyphenols in Rice Bran by Macroporous Resins[J].Scientia Agricultura Sinica, 2016, 49(19): 3818-3830.

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