Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2139-2146.doi: 10.3864/j.issn.0578-1752.2012.11.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Functional Analysis of Resistance Gene SDR1 to Phytophthora sojae in Soybean

 FAN  Su-Jie, WU  Jun-Jiang, CHEN  Chen, WANG  Xin, JIANG  Liang-Yu, WANG  Jin-Sheng, LI  Wen-Bin, XU  Peng-Fei, ZHANG  Shu-Zhen   

  1. 1.东北农业大学大豆研究所/大豆生物学教育部重点实验室，哈尔滨 150030
    2.黑龙江省农业科学院大豆研究所/博士后工作站，哈尔滨 150086
  • Received:2011-12-19 Online:2012-06-01 Published:2012-03-08

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Abstract: 【Objective】 The objective of this research is to obtain resistance-related genes to Phytophthora sojae in soybean, and to provide a theoretical basis for breeding of resistant soybean cultivars.【Method】In previous study, a cDNA library was constructed from the resistant soybean cultivar Suinong 10 in order to identify resistance-related genes in response to P. sojae infection in soybean. An up-regulated EST sharing high sequence similarities with the DR1 genes in other plants was identified and selected in this study. The gene was cloned from Suinong 10 by RT-PCR and constructed to the plant expression vector pCAMBIA3301/SDR1 and transformed into susceptible soybean cultivar Dongnong 50 via Agrobacterium mediated method.【Result】The full-length of this gene was 805 bp, encoding 156 amino acids, with ORF of 471 bp, defined as SDR1 in this study. Three transgenic plants overexpressing SDR1 were obtained and confirmed by real-time PCR, with an increase of gene expression levels by more than 20-fold compared to that of non-transgenic plants, and dot blot assay of DNA of the plants showed that 3 plants had positive hybridization signals. Detached leaf inoculation assay showed significantly increased resistance in the transgenic plants to P. sojae infection. 【Conclusion】The soybean SDR1 was cloned and preliminarily confirmed to confer the resistance to P. sojae by creation and pathogenicity assays of transgenic plants overexpressing the gene.

Key words: soybean, phytophthora sojae, defense-related gene, SDR1, genetic transformation

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