卵丘细胞凋亡与增殖对牛卵母细胞体外发育的影响

doi:10.3864/j.issn.0578-1752.2011.08.020

Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (8): 1702-1709 .doi: 10.3864/j.issn.0578-1752.2011.08.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles Next Articles

Effect of Cumulus Cell Apoptosis and Proliferation on Bovine Oocyte Development in vitro

LI Hai-jun1, JIN Mu-zi2, ZHANG Rui-biao3, DU Chen-guang1, LIU Dong-jun2, CANG Ming2

1.内蒙古农业大学兽医学院
2.内蒙古大学生命科学学院/哺乳动物生殖生物学及生物技术教育部重点实验室
3.包头稀土高新区滨河新区管委会

Received:2011-01-04 Revised:2011-03-10 Online:2011-04-15 Published:2011-04-15
Contact: Dong-Jun liu

Abstract

Abstract:

【Objective】The aim of this study was to investigate the relationship between the appearance of bovine cumulus-oocyte complexes (COCs) and apoptosis or proliferation of cumulus cells and oocyte developmental capacity.【Method】Cumulus-oocyte complexes (COCs) were selected and classified into 4 groups according to oocyte cytoplasm and cumulus status. Apoptosis and cell cycle of cumulus cells from each group were detected by TUNEL staining combined with flow cytometry. Developmental competence was evaluated by development rates at different stages following in vitro fertilization.【Result】Results indicated that, compared to the COCs with five or more layers of cumulus cells and homogeneous ooplasm, that with two-five layers of cumulus cells and slight granulation of the ooplasm showed the highest TUNEL positive ratio of cumulus cells and subsequent embryo developmental potential.【Conclusion】It indicated that apoptosis in cumulus cells is associated with improved developmental potential in bovine immature oocytes, and it makes good markers for embryo development of COCs morphology, apoptosis and the ratio to the stage of G2-M in cumulus cells.

Key words: bovine')">bovine, embryonic development, cumulus cells, apoptosis, proliferation

LI Hai-jun,JIN Mu-zi,ZHANG Rui-biao,DU Chen-guang,LIU Dong-jun,CANG Ming2. Effect of Cumulus Cell Apoptosis and Proliferation on Bovine Oocyte Development in vitro[J].Scientia Agricultura Sinica, 2011, 44(8): 1702-1709 .

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