Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3086-3097 .doi: 10.3864/j.issn.0578-1752.2011.15.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Assessment of Substitution Lines and Identification of QTL Related to Fiber Yield and Quality Traits in BC4F2 and BC4F3 Populations from G.hirsutum ×G.barbadense

LAN  Meng-Jiao, YANG  Ze-Mao, SHI  Yu-Zhen, GE  Rui-Hua, LI  Ai-Guo, ZHANG  Bao-Cai, LI  Jun-Wen, SHANG  Hai-Hong, LIU  Ai-Ying, WANG  Tao, YUAN  You-Lu   

  1.  中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室
  • Received:2010-12-27 Revised:2011-03-02 Online:2011-08-01 Published:2011-03-14
  • Contact: Meng-Jiao LAN E-mail:lanmj100@163.com

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Abstract: 【Objective】 A total of 276 SSR makers were used to assess the substitution lines and identify the QTLs related to fiber yield and quality traits in BC4F2 and BC4F3 populations from Gossypium hirsutum ×Gossypium barbadense with Gossypium hirsutum as recurrent parent, which could lay a foundation for further screening SSSLs (single segment substitution lines) , finely mapping QTL of fiber yield and quality-related traits and realizing molecular assistant breeding. 【Method】 GGT32 was employed to analyze the genotype of BC4F2 and BC4F3 individuals. Possible QTL were identified by one-way analysis of variance on SAS PROC GLM. 【Result】 A total of 50 SSSLs were detected in BC4F2 and BC4F3 individual populations, in which 9 plants contained the single homozygosis chromosome segment of Gossypium barbadense. Twelve plants were screened out with excellent fiber quality trait and a few chromosome segments from Gossypium barbadense. Fifteen QTLs for fiber yield-related traits and 19 QTLs for fiber quality-related traits were mapped in twelve linkage groups, which accounted for 2.80%-14.13% of phenotypic variance. 【Conclusion】 This study indicated that 4 QTLs related to fiber length could be detected in the two populations and 1 QTL for fiber length was reported in previous research, pleiotropism or linked genes existed in some loci that controlled different traits, and not all of additive genes were from the parent with excellent traits.

Key words: G.hirsutum cotton, G.barbadense cotton, Chromosome segment substitution lines, QTL, Fiber yield , Fiber quality traits

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