Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (22): 4746-4753 .doi: 10.3864/j.issn.0578-1752.2010.22.024

• RESEARCH NOTES • Previous Articles     Next Articles

A Proteomic Approach to Study Defense-Related Proteins Responses to GSH Treatment in Pea (Pisum sativum)

LIU Hong-xia, ZHAO Xin, BI Yang, ZHANG Zeng-yan, CHEN Bai-hong, AN Cheng-cai
   

  1. (中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室)
  • Received:2010-06-10 Revised:2010-08-14 Online:2010-11-15 Published:2010-11-15
  • Contact: AN Cheng-cai

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Abstract:

【Objective】 This study was conducted to isolate and identify the defense-related proteins in pea albino seedlings in response to GSH treatment, and to clarify the disease-resistant mechanism of these proteins involved in pea disease response. 【Method】 The method of proteomic analysis was used in the study. Four kinds of defense-related proteins closely related to pea disease response were successfully isolated from pea albino seedlings treated with 50 mmol?L-1 reduced glutathione (GSH) after 48 h of treatment. After the identification with MALDI-TOF MS, a pathogenesis-related protein PR10 (PI49), two ABA responsive proteins ABR17 and ABR18, and a glycine-rich RNA binding protein (GRPs) were identified. The possible roles of these four proteins in the disease response of pea seedlings to GSH treatment were discussed. 【Result】 Results showed that ABR17 and PI49 emerged concomitantly, indicating a potential cooperative effects of these two proteins in pea disease response to enhanced disease-resistance of pea. 【Conclusion】 Under GSH treatment, PR and ABA responsive proteins were the main anti-fungal proteins at 48 h after treatment, indicating that these two proteins would play pivotal roles in pea disease response to pathogen attacks.

Key words: pea (Pisum sativum), pathogenesis-related protein, 2-DE, MALDI-TOF MS, GSH

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