Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 990-998.doi: 10.3864/j.issn.0578-1752.2012.05.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Comparison of the In vitro Antiviral Activity Between the Chicken Mutant-Type and Wild-Type Mx Proteins

 FU  De-Zhi, SUN  Min, ZHENG  Meng-Meng, GAO  Bo, ZHANG  Ya-Ni, DOU  Tao-Cun, WANG  Ke-Hua, LI  Bi-Chun, SUN  Huai-Chang   

  1. 1.扬州大学动物科学与技术学院/江苏省动物遗传繁育与分子设计重点实验室，江苏扬州 225009
    2. 扬州大学兽医学院, 江苏扬州 225009
    3.中国农业科学院家禽研究所，江苏扬州 225001
  • Received:2011-06-23 Online:2012-03-01 Published:2012-01-09

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Abstract: 【Objective】This experiment was conducted to assess the relationship between the 631 amino acid variations of chicken Mx protein and the disease resistance. 【Method】 Mutant pcDNA3.0-MMx and wild-type pcDNA3.0-Mx plasmids of Mx protein gene of Chinese Langshan chicken were constructed successfully, then the CEF and NIH-3T3 cells were transfected by mutant pcDNA3.0-MMx and wild-type pcDNA3.0-Mx plasmids, respectively. RT-PCR was used to identify the expression of mutant MMx and wild-type Mx genes and the mini-cytopathic effect inhibition assay was used to evaluate the antiviral activity for Newcastle disease virus (NDV) and vesicular stomatitis virus (VSV). 【Result】 The recombinant Mx protein protected the CEF cells from NDV infection up to 48-h incubation while the NIH-3T3 cells transfected by pcDNA3.0-MMx were not infected by 60-h incubation with VSV, however, the CEF and NIH-3T3 cells transfected by pcDNA3.0-Mx developed pathological changes during 24-h incubation with the viruses. 【Conclusion】 Recombinant Mx protein could delay the infection of NDV and VSV at single-cell level.

Key words: Mx protein, antiviral activity, NDV, VSV, Langshan chicken

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