基于13C脉冲标记揭示施氮对牧草光合碳分配的影响

doi:10.3864/j.issn.0578-1752.2025.21.004

Abstract

Abstract:

【Objective】Field experiments were conducted to examine the effects of nitrogen fertilization on the biomass yields of alfalfa and oat by measuring the plant’s ¹³C fixation and the ¹³C abundance in different plant organs, aiming to shed light on the transport and distribution of photosynthetic carbon in the plant-soil system and to explore effective means of soil carbon sequestration and soil quality improvement. 【Method】The ¹³C pulse labeling method was adopted in the field experiment. Two treatments were incorporated for alfalfa and oat crops, including nitrogen fertilization at 75 kg·hm^-2 in addition to a control experiment (no nitrogen). Plant aboveground and belowground biomass were measured. ¹³C abundances in different plant organs were also measured. 【Result】Nitrogen fertilization increased soil organic carbon (SOC) content in oat plots, while no significant SOC effects were observed in alfalfa plots. However, nitrogen fertilization increased the leaf, stem, and root biomass in alfalfa by 117.5% for leaf and stem and 97.8% for roots. The biomass increases in oat under nitrogen fertilization were smaller than that in alfalfa. The aboveground and belowground biomass under nitrogen fertilization in oat was increased by 19.1% and 9.6%, respectively, compared with the control experiment. Moreover, nitrogen fertilization also increased ¹³C abundance in alfalfa and oat by 54.36‰ and 28.6‰, respectively. Additionally, more carbon was allocated to roots in alfalfa under nitrogen fertilization than the control experiment (11.5% versus 5%). No significant differences in carbon allocation were observed in oat, despite slight increases in root carbon allocation in oat. 【Conclusion】Nitrogen fertilization increased soil organic carbon content of alfalfa and oat, increased alfalfa biomass yield, and stimulated carbon allocation to roots in alfalfa. Nevertheless, nitrogen fertilization showed no significant effects on both carbon fixation and allocation in oat.

Key words: ¹³C pulse labeling, nitrogen fertilizer, alfalfa, oat, carbon allocation

XU XiuYuan, ZHANG HongZhi, XU LiJun, XUE Wei, NIE YingYing, GUO MingYing, LI JinXia, ZHAO YaRu, SHI MingJiang. Effects of Nitrogen Fertilization on Photosynthetic Carbon Allocation in Pasture Based on ¹³C Pulse-Labeling Experiments[J].Scientia Agricultura Sinica, 2025, 58(21): 4346-4356.

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土层 Soil layer (cm)	处理 Treatment	土壤有机碳 SOC (g·kg^-1)	pH	黏粒 Clay (%)	粉粒 Silt (%)	砂粒 Sand (%)
0-10	A1	19.90±1.33Ba	7.40±0.27Aa	5.13±0.26Aa	47.23±2.29Aa	47.64±2.55Aa
	A2	19.95±1.35Ba	7.67±0.47Aa	4.88±0.32Aa	46.99±0.68Aa	48.15±1.00Aa
	B1	25.68±0.46Aa	6.95±0.09Ab	5.34±0.06Aa	45.90±1.11Ab	48.77±1.05Aab
	B2	25.45±0.23Aa	7.17±0.12Aa	4.84±0.07Aa	50.08±2.03Aa	45.09±1.95Aa
10-20	A1	19.28±0.92Ba	7.55±0.56Aa	4.33±0.32Aa	44.90±2.75Aa	50.78±3.06Aa
	A2	20.83±2.23Ba	7.84±0.82Aa	4.84±0.31Aa	47.17±3.99Aa	47.99±4.31Aa
	B1	28.13±0.53ABa	8.08±0.09Aa	5.21±0.13Aa	53.90±0.71Aa	40.89±0.58Aa
	B2	23.86±0.24Ab	7.44±0.68Aa	4.75±0.21Aa	51.63±0.93Aa	43.63±1.14Aa
20-30	A1	16.95±2.22Aa	7.36±0.28Aa	4.47±0.36Aa	44.82±0.42Aa	50.71±0.78Aa
	A2	17.41±0.50Aa	7.66±0.64Aa	4.88±0.36Aa	42.82±0.33Aa	52.31±0.03Aa
	B1	20.31±4.74Aa	7.00±0.95Ab	3.59±0.57Ab	43.10±2.57Ab	53.31±3.14Ab
	B2	19.93±0.46Ac	7.19±0.05Aa	4.68±0.68Aa	43.90±5.75Aa	51.42±6.43Aa

Effects of Nitrogen Fertilization on Photosynthetic Carbon Allocation in Pasture Based on ¹³C Pulse-Labeling Experiments

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Effects of Nitrogen Fertilization on Photosynthetic Carbon Allocation in Pasture Based on 13C Pulse-Labeling Experiments

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Effects of Nitrogen Fertilization on Photosynthetic Carbon Allocation in Pasture Based on ¹³C Pulse-Labeling Experiments