Abstract:

【Objective】 The method for constructing core collection of Malus sieversii based on molecular markers data was proposed. 【Method】 According to 128 SSR allele of 109 M. sieversii, an allele preferred sampling strategy was used to construct M. sieversii core collection using UPGMA cluster method according to Nei & Li, SM and Jaccard genetic distances by stepwise clustering and compared with the random sampling strategy. The number of lost allele and t-test of Nei's gene diversity and Shannon's Information index were used to evaluate representative of core collections. 【Result】 Compared with the random sampling strategy, allele preferred sampling stragegy could construct more representative core collections. SM, Jaccard and Nei & Li genetic distance had not distinct difference for construction of M. sieversii core collection. SRAP data and morphological data showed that allele preferred sampling strategy was a good sampling strategy for constructing core collection of M. sieversii.【Conclusion】 Allele preferred sampling strategy combined with SM, Jaccard and Nei & Li genetic distances using stepwise clustering is a suitable method for constructing M. sieversii core collection.

Key words: Malus sieversii, core collection, allele preferred sampling strategy, genetic distance, SSR markers, SRAP markers