Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (5): 882-888 .

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Isolating Soil Drought-induced Genes from maize Seedlings leaves through Suppression Subtractive Hybridization

  

  1. 中国农业科学院作物科学研究所
  • Received:2006-03-22 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10

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Abstract: A forward SSH library was constructed to investigate gene expression profiles, using seedling leaves of “CN165”, a drought-tolerant maize inbred line. In the SSH library, 672 positive clones were picked up randomly. After PCR of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster anslysis of the ESTs sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, eight uniESTs were unknown proteins and putative proteins, and four uniESTs had not protein homology. Many drought stress-induced genes involving many metabolism pathways were found by this approach.

Key words: maize seedings, drought stress, suppression subtractive hybridization(SSH), drought-tolerant genes

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