Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (01): 70-75 .

• PLANT PROTECTION • Previous Articles     Next Articles

Analysis on Genetic Relationship of Oxya chinensis and O. japonica from Xuzhou and Pingshan

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  1. 山西大学生命科学与技术学院
  • Received:2004-07-26 Revised:1900-01-01 Online:2005-01-10 Published:2005-01-10

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Abstract: Genetic Relationships among Oxya chinensis and O. japonica populations collected from Xuzhou City, Jiangsu Province and Pingshan County , Hebei Province were analyzed by using random amplified polymorphic DNA (RAPD) markers. 125 clear and stable bands of 43 grasshopper individuals with molecular weight ranging from 200 to 2 200 bps revealed on the electropherogram amplified by 10 random primers,and 123 (99%) of this bands were polymorphic, Shannon 's index showed that the genetic diversity within O. chinensis(0.3432) was more higher than that of O. japonica (0.2781 ). Nei's genetic distance of O. chinensis and O. japonica from same area was less than that from two far apart areas. The dendrogram based on Nei's genetic distance of RAPD markers was constructed with UPGMA and Neighbor-Joining methods. Cluster analysis indicated that all the individuals were grouped into two main clusters,namely, O. chinensis from Xuzhou and Pingshan has been clustered into one cluster,while O. japonica from the two regions belonged to the other cluster. The results showed that RAPD can provide polymorphisms even at slight difference leval among individuals, indicated that RAPD is an effective molecular marker to distinguish the closed related species.

Key words: Oxya chinensis, Oxya japonica, RAPD, Genetic relationship

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