Abstract: 【Objective】 β-glucosidase (BG, 4-β-D-glueosidase) is a kind of hydrolase which hydrolyzes glycosidic bond from glycopolymer or oligosaccharide to release non reducing sugar group, which plays an important role in controlling anther dehiscence. The anthers of the photoperiod-temperature sensitive genic male sterile (PTGMS) wheat line BS366 did not dehiscence in sterile environment, but completely or partially cracked in fertile environment. In order to study the regulatory function of BG gene on anther dehiscence in wheat, the TaBG was cloned from BS366 and its potential function in anther dehiscence was analyzed, which provided theoretical basis for further analysis of molecular mechanism of abnormal anther dehiscence in photoperiod thermo sensitive male sterile line of wheat.【Method】TaBG was cloned from the anther of PTGMS line BS366, and the primary structure, secondary structure, tertiary structure of TaBG protein were predicted. In addition, the cis-acting elements in promoter region prediction, phylogenetic tree construction with other species, interaction between miRNA and TaBG were performed using bioinformatics software. The expression levels of TaBG and the interacted tae-miR395a in anthers and glumes under MeJA and SA treatment were determined. 【Result】 The total length of TaBG was 1473 bp, which encoded 490 amino acids, and the theoretical PI was 8.12, belonging to the glycosylhydrolase superfamily. The results showed that TaBG may be regulated by stress resistance-related miRNAs, such as miR169 and miR395a. Based on the prediction of protein interaction, it was found that TaBG might interact with glucose-methanol-choline oxidoreductase (GMC) and endoglucanase (EG). TaBG is located in the liquid bubble of the wheat proton. The qPCR results showed that the expression of TaBG in Bilocular stage (stage 13), Dehiscence (stage14) and Senescence (stage15) showed an upward and then downward trend. TaBG was the highest expression in Dehiscence, and the expression of TaBG in No dehiscenc of anther was 2.8 times higher than that of Complete Dehiscenc of anther. After treatment of MeJA, the expression of TaBG in anther and glume showed dowenward. The tae-miR395a expression pattern was the opposite.【Conclusion】 The expression of TaBG in the sterile anther was higher than that in the fertile environment. High expression of TaBG could increase the content of soluble sugar in the anther under the sterile environment, and then increase the osmotic potential in the anther, so as to slow down the dehydration of anther dehiscence. This study will lay a foundation for elucidating the biological function of BG in regulating anther dehiscence.

Key words: wheat, 4-β-D-glueosidase, miRNA, anther dehiscence