Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (06): 1159-1165 .doi: 10.3864/j.issn.0578-1752.at-2006-7062

• PLANT PROTECTION • Previous Articles     Next Articles

ISSR Marker and ITS Sequence Study of Melampsora larici-populina

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  • Received:2006-01-17 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10

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Abstract: 【Objective】An experiment was condnced to compare the differences of ITS-nrDNA lied between the China isolates and foreign isolates of Melampsora larici-populina. 【Method】ITS sequences and ISSR markers based on 11 isolates of 5 races collected from different parts in China were studied. 【Results】There were no differences in the ITS sequence among 11 China isolates. ITS sequence of China isolates was more homogeneous with that of UK than that of France, German and Canada. ISSR marker study showed that the tested 11 isolates can be divided into western population and northern population. Genetic diversity index of race 2 was significantly different from race 4, race 3 and race 1, No significant difference was found among the other races. Race origination division was not harmonize with its genetic division, except race 2. 【Conclusion】ITS region is conservative, and ITS sequence was not fit for studying differences among races. ISSR marker can be used for intra-species population study, and Melampsora larici-populina in China can be divided into two populations.

Key words: Melampsora larici-populina, Race, ITS sequence, ISSR marker

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