Abstract: 【Objective】The aim of the study was to explore the effects of genetic variation of ADIPOQ on growth traits of sheep, and to find the molecular genetic markers related to growth traits in Ningxia high-quality mutton sheep breeding, so as to achieve the purpose of molecular assisted breeding. 【Method】Firstly, the mutation sites of ADIPOQ in Dupo sheep, Tan sheep and small-tailed Han sheep were obtained by Allegro Targeted Genotyping. At the same time, ear tissues of 383 different hybrid progenies of three breeds were collected. The SNPs were genotyped by Sequenom Mass ARRAY?SNP. Haploview was used to analysis linkage disequilibrium and construct haplotypes of the polymorphic loci. Association analysis between the SNPs in ADIPOQ with growth traits of newborn and 3-month-old sheep.【Result】A total of 7 SNPs were screened, and 7 SNPs showed polymorphism in the hybrid population. The dominant genotypes of SNP1-SNP7 were CC, GG, GG, CT, AG, GG and AA, and the dominant alleles were C, G, G, C, G, G and A. X² test showed that all loci were in Hardy Weinberg equilibrium (except SNP7 site deviated from the equilibrium in all individuals). SNP1 (except F2), SNP4, SNP5 and SNP6 were moderately polymorphic (0.25 ≤ PIC < 0.50), SNP2 and SNP3 were moderately polymorphic (0.25 ≤ PIC < 0.50) in F2 and SNP7 were moderately polymorphic (0.25 ≤PIC < 0.50) in H1, and were low polymorphic (PIC < 0.25) in other populations. The results of linkage imbalance analysis showed that SNP2-SNP3 and SNP5-SNP6 formed two strong linkage, each of which constructed three haplotypes, and formed 4 and 6 genotypes respectively after combination, among which the dominant genotype was H1H1 and H4H6. 13 genotypes were produced after the haplotypes formed by SNP2-SNP3 and SNP5-SNP6 were recombined, and the dominant genotype was H1H1H4H6. Single SNP correlation analysis revealed that: SNP1 site in F1 population, the primary chest of GG genotype was significantly higher than that of CG genotype(P<0.05), and in H2 population, the primary body length of CC genotype was significantly higher than that of GG genotype(P<0.05). SNP2 site in H1 population, the primary body height of CC genotype was significantly lower than that of CG and GG genotypes (P < 0.05). SNP3 site in F1 population, the three months body weight of AG genotype was significantly higher than that of GG genotype, and in H1 population, the primary body height of AA genotype was significantly lower than AG and GG genotypes (P<0.05). SNP4 site in F1 population, the three months weight of CC genotype was significantly higher than that of CT and TT genotypes(P<0.05), in F2 population, the primary weight and primary chest of CT genotype were significantly higher than that of TT genotype(P<0.05). and that of TT genotype was significantly lower than that of CC and CT genotype(P<0.05) in H1 population, and in H2 population, the primary body height and primary body length of TT genotype were significantly higher than that of CT genotype(P<0.05). SNP5 site in F2 population, AA genotype had significantly higher primary body height and primary body length than GG genotype(P<0.05). There were significant differences in primary weight, body height, body length, three months body height and chest among different genotypes of SNP6(P<0.05). SNP7 site in H2 population, AA genotype had significantly higher primary body length than GA genotype(P<0.05).When combined with all groups, we found that , the primary weight and body length of CG genotype at SNP2 were significantly higher than GG genotype(P<0.05), the primary weight of TT genotype at SNP4 was significantly lower than CC and CT genotypes(P<0.05), the primary weight of AA genotype at SNP6 was significantly higher than GG and GA genotype(P<0.05), and the primary chest was significantly higher than GG genotypes(P<0.05). There was no significant difference in growth traits among the other five genotypes(P>0.05). The results of haplotype association analysis showed that the primary weight and primary body length of H2H3 genotype were significantly higher than those of other genotypes(P<0.05), while the primary weight of H5H5 genotype was significantly higher than that of H5H6 and H6H6(P<0.05), the primary body height was significantly higher than that of H5H6(P<0.05), the primary chest was significantly higher than that of H6H6(P<0.05), and the three months weight of H4H4 genotype was significantly higher than that of H5H5 genotype(P<0.05), the body weight and body length at three months were significantly higher than those of H4H6 genotype(P<0.05), and the chest of three months was significantly higher than that of H4H5,H5H6 and H5H5 genotype(P<0.05). After SNP2-SNP3 and SNP5-SNP6 haplotypes were recombined, the primary weight, body height, body length and chest of H2H3H4H4 genotype were the highest, which were different from other genotypes. The body length of H1H2H4H4 and H2H2H4H4 genotypes(P<0.05).【Conclusion】The results showed that the different SNPs and combined genotypes in ADIPOQ had effect on sheep different growth traits, the seven SNPs in this study can be used as potential molecular markers for growth traits in Ningxia high quality mutton sheep breeding.

Key words: sheep, ADIPOQ, polymorphisms, growth traits, haplotype