Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (20): 4115-4124.doi: 10.3864/j.issn.0578-1752.2023.20.015

• ANIMAL SCIENCE·VETERINARY SCIENCE • Previous Articles     Next Articles

Fatty Liver Model Construction and Its Effectiveness Evaluation Induced by Dexamethasone in Broilers

WANG ChaoHui(), SUN Xi, WANG QiangGang, LIU RuiBing, LI Ni, YANG XiaoJun, LIU YanLi()   

  1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi
  • Received:2022-12-20 Accepted:2023-05-30 Online:2023-10-16 Published:2023-10-31
  • Contact: LIU YanLi

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Abstract:

【Background】Excessive abdominal fat deposition and fatty liver syndrome are important industrial problems of lipid metabolism disorder in poultry breeding. The liver is the main site of de novo fatty acid synthesis in poultry, which plays a vital function in nutrients metabolism, formation of bile acids and detoxication. Therefore, the mechanism analysis of lipid metabolism disorder will provide reference for the healthy development of poultry industry, but the disease model is necessary for pathogenesis research. 【Objective】This experiment was conducted to evaluate the effect of dexamethasone-induced fatty liver in broilers, aiming to provide the reference for the construction of lipid metabolism disorder model in poultry. 【Method】Sixteen 35-day-old AA broilers with similar body weight were randomly divided into control group and dexamethasone group (DXM), with 8 replicates in each group. The birds in the DXM were injected subcutaneously with dexamethasone sodium phosphate (4.5 mg·kg-1) for 7 days, and the birds in the control group were given the administration of normal saline injection. After 7 days, oil red O staining was used to analyze the histopathological changes of the liver, and serum biochemical and antioxidant indexes were detected. RT-PCR was applied to detect genes expression related to lipid metabolism and inflammatory response in the liver. 【Result】Compared with the control group, the liver index of broilers in the DXM was significantly increased (P<0.05). Oil red O sections showed a large number of red lipid droplets, and the contents of TG and TC in the liver were higher than those in the control group (P<0.05). Serum biochemical results showed that aspartate amino transferase (AST), total bilirubin (TBIL), uric acid (UA), total protein (TP), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), total cholesterol (TC) and triglyceride (TG) levels in the DXM were significantly higher than those in the control group (P<0.05), but there was no significant difference about alanine aminotransferase (ALT) and glucose (GLU) (P>0.05). Dexamethasone injection significantly increased genes expression about lipid synthesis, such as ACC, FAS, SCD1, PPARγ, ChREBP, SREBP-1c, IGF2 and GR in the liver (P<0.05), but ELOVL6 expression was not affected (P>0.05). In addition, genes expression about lipid catabolism such as CPT1, LPL and PPARα were deceased in DXM group (P<0.05). However, the expression of ATGL gene related to lipid hydrolysis, CETP and MTTP gene related to lipid transport were significantly higher in DXM group (P<0.05). On the other hand, DXM injection significantly increased the genes expression of MyD88, NFκB, IL-6, TNF-α in the liver of broilers (P<0.05). Antioxidant analysis showed that there was no significant change in the total antioxidant capacity of liver and serum (P>0.05), but the content of malondialdehyde in serum was significantly higher in DXM group (P<0.05). 【Conclusion】Neck subcutaneous injection of DXM could cause lipid metabolism disorders in broilers, and lead to oxidative stress and liver inflammation, which was similar to the characteristics of fatty liver disease, indicating that this method could be used to establish the fatty liver model in broilers quickly.

Key words: dexamethasone, broiler, fatty liver syndrome

Table 1

The primer sequences"

引物 Primer 登录号 Accession number 引物序列 Primer sequences (5′ - 3′) 产物大小 Product size(bp)
β-actin L_08165 F:ATTGTCCACCGCAAATGCTTC
R:AAATAAAGCCATGCCAATCTCGTC		 113
ACC XM_046929960 F:GCTTCCCATTTGCCGTCCTA
R:GCCATTCTCACCACCTGATTACTG		 185
FAS NM_205155 F:TTTGGTGGTTCGAGGTGGTA
R:CAAAGGTTGTATTTCGGGAGC		 212
SCD1 NM_204890 F:GTTTCCACAACTACCACCATACATT
R:CCATCTCCAGTCCGCATTTT		 175
PPARγ NM_001001460 F:CCAAGGCAGCGGCAAAATAA
R:GTGCCCATAAATGATGGCCTAA		 188
ELOVL6 XM_046916529 F:GGTGGTCGGCACCTAATGAA
R:TCTGGTCACACACTGACTGC		 169
ChREBP EU_152408 F: ATTGACCCGACCCTGACG
R: CATACTGGATGTACCACGCTCT		 160
SREBP1c XM_046927256 F: GCCCTCTGTGCCTTTGTCTTC
R: ACTCAGCCATGATGCTTCTTC		 130
IGF2 NM_001030342 F: ATTGTCCACCGCAAATGCTTC
R: AAATAAAGCCATGCCAATCTCGTC		 131
GR NM_001037826 F: TCCTGTGCTTTGCACCAGAT
R: GATGGCCTTTCCCAGCTCTT		 245
ATGL NM_001113291 F: TCCTAGGGGCCTACCACATC
R: CCAGGAACCTCTTTCGTGCT		 195
CETP NM_001034814 F: AGTCTCGCCCTTCCTGAGAT
R: GCAGCTTGGATAGTGACCGT		 149
CPT1 XM_046918285 F: TAGAGGGCGTGGACCAATAA
R: TGGGATGCGGGAGGTATT		 229
LPL NM_205282 F: CCGATCCCGAAGCTGAGATG
R: ACATTCCTGTCACCGTCCAC		 186
MTTP NM_001109784 F: GCAGATGGACAGAGTTGGCT
R: ACACCAAAAGTGCAAGGTGC		 224
PPARα XM_046906400 F: TTTAACGGAGTTCCAATCGC
R: AACCCTTACAACCTTCACAAGC		 224
IL-6 NM_204628 F: CTCGTCCGGAACAACCTCAA
R: GGAGAGCTTCGTCAGGCATT		 96
MyD88 NM_001030962 F: CGTGCCAAAGACTTCAGAGC
R: CTGGCAAGACATCCCGATCA		 191
NFκB NM_001396038 F: CAGCCCATCTATGACAACCG
R: CAGCCCAGAAACGAACCTC		 151
TNFα XM_046927265 F: TGTGTATGTGCAGCAACCCGTAGT
R: GGCATTGCAATTTGGACAGAAGT		 229

Fig. 1

Effects of dexamethasone on hepatic histopathology, TG and TC content in broilers"

Table 2

Effects of dexamethasone on serum biochemical indices of broilers"

项目 Item 对照组 Control 地塞米松组Dexamethasone P值 P-value
谷丙转氨酶ALT（U·L-1） 0.86±0.34 2.00±0.53 0.096
谷草转氨酶AST（U·L-1） 317.33±14.72 466.43±52.36* 0.029
总胆红素TBIL（µmol·L-1） 20.13±1.06 34.14±2.45** <0.001
尿酸UA（µmol·L-1） 318.43±37.52 532.86±48.32** 0.004
血清总蛋白TP（g·L-1） 30.44±1.42 45.00±1.30** <0.001
血清葡萄糖GLU（mmol·L-1） 12.11±0.25 15.04±1.29 0.063
高密度脂蛋白胆固醇HDL-c（mmol·L-1） 1.71±0.09 4.42±0.21** <0.001
低密度脂蛋白胆固醇LDL-c（mmol·L-1） 0.48±0.26 1.53±0.14** <0.001
总胆固醇TC（mmol·L-1） 2.75±0.14 7.93±0.34** <0.001
甘油三酯TG（mmol·L-1） 0.65±0.05 1.54±0.28* 0.018

Fig. 2

Effects of dexamethasone on lipid metabolism related gene expression in broilers"

Fig. 3

Effects of dexamethasone on expression of genes related to liver inflammation in broilers"

Fig. 4

Effects of dexamethasone on antioxidant indices in serum and liver of broilers"

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