Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (13): 2431-2442.doi: 10.3864/j.issn.0578-1752.2023.13.001


The Genetic Basis of Flavonoid Contents in Wheat and Its Application in Functional Wheat Variety Breeding

CHEN Jie(), CHEN Wei   

  1. College of Plant Science and Technology, Huazhong Agricultural University/National Key Laboratory of Crop Genetics and Improvement, Wuhan 430070
  • Received:2023-03-29 Accepted:2023-05-10 Online:2023-07-01 Published:2023-07-06


Accompanying the elevated expenses on consumption, people’s urge upon food has been gradually changed from “eat to be fed” to “eat to be satisfied” and further to “eat to gain nutrition” and “eat to be healthy”. Accordingly, breeders considered the wheat breeding goals should be set as breeding wheat with better quality along with higher yield, wherein the phrase “functional wheat variety” was recently raised. Flavonoids comprise one of the most widely reported categories of metabolites, the contents of which have been included within the “functional wheat variety” breeding program for its connection with plant phenotypes and its contribution to human health. The combination of metabolomics approach and genetics design has been proved to be efficient in identifying the candidates that responsible for metabolite contents, that said its application in wheat was lagged behind due to the lately released wheat reference genome. Further, the deficient knowledge upon the genetic basis of metabolites has in turn constrained the application of breeding “functional wheat variety”. In the current manuscript, the research progresses on genetic basis of flavonoids are briefly summarized, and its application for wheat breeding is highlighted. Meanwhile, the metabolomics-assisted breeding frame is concepted. Ultimately, the “functional wheat variety” breeding program will be achieved through the combination of the fundamental researches and breeding applications.

Key words: flavonoid, functional wheat, genetic basis, breeding

Fig. 1

Diversity on the flavonoid skeletons A: Main sub-categories of flavonoid; B: Respective flavonoids with various hydroxyl moieties on the B-ring"

Table 1

Wheat homologs against the rice flavonoid biosynthetic pathway genes"

Wheat homologs
OsCGT AK071127[72] C-糖基转移酶CGT 7D03G0485300 7A03G0502700 7B03G0320000
CYP93G1 AK100972[73] 黄酮合酶 FNS 2B03G0120700 2D03G0079000 2A03G0084500
ROMT-9 DQ288259[74] 甲基转移酶 OMT 7D03G0805700 7A03G0821400 7B03G0680100
CYP75B4 AK070442[59] 羟化酶F3′ 5′H 7A03G0998300 7B03G0834400 7D03G0954300
DFR AB003496[75] 二氢黄酮醇还原酶DFR 3D03G0544800 3B03G0671600 3A03G0590800
CYP75B3 NP_001064338[76] 羟化酶F3′H 1B03G1273800 1D03G1038800 1A03G1079300
ROMT-15 XM_483167[77] 甲基转移酶OMT 7A03G0552300 7D03G0534100 7B03G0369400
ROMT-17 XM_507282[77] 甲基转移酶OMT 7B03G0369800 7D03G0535200 7A03G0552500
OsFNS I NM_001055334[78] 黄酮合酶FNS 4D03G0794300 4B03G0902300
OsFLS Os02g52840[60] 黄酮醇合酶FLS 6D03G0724900 6A03G0855700 6B03G1024000

Fig. 2

Flowchart of identifying candidates that affect metabolite contents and constructing the metabolite-phenotype network"

Fig. 3

Scheme of the metabolomics-assisted wheat breeding This scheme is exemplified by breeding wheat with high tricin contents, wherein metabolomics methods, hybridization protocols and the fast-breeding systems are combined. The elevated tricin content would be achieved without knowing key responsible genes as a premise. Meanwhile, the candidate for tricin content will be promisingly identified by discriminating the donor (which harbors high tricin content) chromosomal segments through molecular markers"

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