Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (23): 4423-4428.doi: 10.3864/j.issn.0578-1752.2019.23.023

• SPECIAL FOCUS: PREVENTION AND CONTROL OF WATERFOWL INFECTIOUS DISEASE • Previous Articles     Next Articles

Correlation Research of the Serological Test (HI) and Challenge Test of Duck Tembusu Virus Inactivated Vaccine

WANG XiaoLei,LIN Jian,YANG ZhiYuan,LIU LiXin,DUAN HuiJuan,CHENG HuiMin,ZHAO JiCheng,PAN Jie,LIU YueHuan()   

  1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097
  • Received:2019-04-17 Accepted:2019-08-23 Online:2019-12-01 Published:2019-12-01
  • Contact: YueHuan LIU E-mail:liuyuehuan@sina.com

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Abstract:

【Objective】The objective of this paper was to evaluate the correlation of the serological test (HI) and challenge test of duck Tembusu virus inactivated vaccine.【Method】Three batches of vaccines ( laboratory trial products) were used to immunize the 51-day-old young ducks and 260-day-old laying ducks by intramuscular injection in 0.15, 0.3, 0.5 and 1.0 mL, respectively. Secondary immunization was proceeded in the same doses and route at 14 day after primary immunization. Ducks were regrouped according to the HI titer and challenged with 0.5 mL (100 DID50) DTMUV at day 28 after secondary immunization. Serum samples were collected at 2 days later for virus isolation and laying ducks were dissected at 8 days later to observe the ovarian lesions. All the results were counted to evaluate the immune potency of vaccines. The correlation of HI titer and immune protection rate was analyzed.【Result】The protection rate of immunized young ducks with HI titer of 1:5 or lower, 1:10, 1:20, 1:40, 1:80, 1:160, 1:320, 1:640 was 50% (20/40), 43.8% (7/16), 74.3% (26/35), 86.7% (26/30), 96.6% (57/59), 92.7% (51/55), 95% (38/40) and 100% (11/11), respectively. The protection rate was 48.2% with HI titer lower than 1:20, while the rate was 74.3% with HI titer of 1:20 and 93.8% with HI titer of 1:40 or greater. In laying ducks, the protection rate of flocks with HI titer of 1:5 or lower, 1:20, 1:40 and 1:80 was 66.7% (12/18), 83.3% (10/12), 95.2% (20/21) and 100% (15/15), respectively. The protected proportion of ducks with HI titer of 1:10, 1:160, 1:320 and 1:640 was 2/3, 7/7, 1/1 and 2/2, respectively. The protection rate was 66.7% with HI titer of 1:10 or below, while the rate was 83.3% with HI titer of 1:20 and 97.8% with HI titer of 1:40 or greater. There were significant positive correlations between the HI titer and the immune protection rate both in young ducks and laying ducks. The Spearman’s correlation coefficient was 0.905 (P<0.01) and 0.932 (P<0.01), respectively. 【Conclusion】There were positive correlations between the HI titer and the immune protection rate of duck Tembusu virus inactivated vaccine (strain HB). The HI detection could be used to evaluate the efficacy of duck Tembusu virus inactivated vaccine (strain HB) instead of challenge test. The HI titer of 1:20 could be defined as the criteria of protection for the duck Tembusu virus inactivated vaccine after immunization.

Key words: duck Tembusu virus, HI test, antibody titer, challenge, correlation

Table 1

Batchs of vaccine, dose and No. of immunization"

疫苗
Vaccines		 免疫鸭日龄
Day age of immunized ducks		 免疫剂量（mL）
Doses of immunization		 免疫途径
Immunization route		 免疫鸭数量
No. of immunized ducks
实验室制品01
Laboratory trial products 01		 51 0.15 / 0.3 / 0.5 / 1 胸肌注射
Intramuscular injection		 100 (25 / 25 / 25 / 25)
260 0.3 / 0.5 / 1 30 (10 / 10 / 10)
实验室制品02
Laboratory trial products 02		 51 0.15 / 0.3 / 0.5 / 1 100 (25 / 25 / 25 / 25)
260 0.3 / 0.5 / 1 30 (10 / 10 / 10)
实验室制品03
Laboratory trial products 03		 51 0.15 / 0.3 / 0.5 / 1 96 (24 / 24 / 24 / 24)
260 0.3 / 0.5 / 1 30 (10 / 10 / 10)
对照组 51 / / 40
Control 260 / / 20

Table 2

HI titer and immune efficiency in young ducks and laying ducks"

分组
Groups		 青年鸭保护率（%）
Protection rate of young ducks		 产蛋鸭保护率（%）
Protection rate of laying ducks
≤1:5 50 (20A / 40B) 66.7 (12 / 18)
1:10 43.8 (7 / 16) 2 / 3
1:20 74.3 (26 / 35) 83.3 (10 / 12)
1:40 86.7 (26 / 30) 95.2 (20 / 21)
1:80 96.6 (57 / 59) 100 (15 / 15)
1:160 92.7 (51 / 55) 7 / 7
1:320 95 (38 / 40) 1 / 1
1:640 100 (11 / 11) 2 / 2
合计
Total		 82.5 (236 / 286) 87.3 (69 / 79)
对照组
Control		 0% (0 / 40) 10% (2 / 20)
[1] SU J, LI S, HU X, YU X, WANG Y, LIU P, LU X, ZHANG G, HU X, LIU D, LI X, SU W, LU H, MOK N S, WANG P, WANG M, TIAN K, GAO G F . Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLoS ONE, 2011,6(3):e18106.
doi: 10.1371/journal.pone.0018106 pmid: 21455312
[2] CAO Z, ZHANG C, LIU Y, LIU Y, YE W, HAN J, MA G, ZHANG D, XU F, GAO X, TANG Y, SHI S, WAN C, ZHANG C, HE B, YANG M, LU X, HUANG Y, DIAO Y, MA X, ZHANG D . Tembusu virus in ducks, China. Emerging Infectious Diseases, 2011,17(10):1873-1875.
doi: 10.3201/eid1710.101890
[3] YAN P, ZHAO Y, ZHANG X, XU D, DAI X, TENG Q, YAN L, ZHOU J, JI X, ZHANG S, LIU G, ZHOU Y, KAWAOKA Y, TONG G, LI Z . An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China. Virology, 2011,417(1):1-8.
doi: 10.1016/j.virol.2011.06.003
[4] 刘建涛, 孙法良, 杨同涛, 崔京腾, 宁明刚 . 再谈鸭黄病毒病. 家禽科学, 2017(9):44-46.
LIU J T, SUN F L, YANG T T, CUI J T, NING M G . Re-discussion on duck flavivirus disease. Poultry Science, 2017(9):44-46. (in Chinese).
[5] 张大丙 . 鸭出血性卵巢炎的研究进展. 中国家禽, 2011,33(14):37-38.
ZHANG D B . Research progress of duck hemorrhagic ovaritis. China Poultry, 2011,33(14):37-38. (in Chinese).
[6] LIN J, LIU Y, WANG X, YANG B, HE P, YANG Z, DUAN H, XIE J, ZOU L, ZHAO J . Efficacy evaluation of an inactivated duck Tembusu virus vaccine. Avian Diseases, 2015,59(2):244-248.
doi: 10.1637/10960-101514-Reg pmid: 26473674
[7] LI G, GAO X, XIAO Y, LIU S, SHAN P, LI X, YING S, ZHANG Y, LEI Y, WU X . Development of a live attenuated vaccine candidate against duck Tembusu viral disease. Virology, 2014, 450-451(1):233-242.
doi: 10.1016/j.vetmic.2019.03.022 pmid: 30955813
[8] 张欣 . 坦布苏病毒SDS-70株弱毒苗的制备及安全性与免疫效力的初步评价[D]. 泰安: 山东农业大学, 2017.
ZHANG X . The preparation, safety and efficacy study of an attenuated live SDS-70 duck Tembusu virus strain[D]. Taian: Shandong Agricultural University, 2017. (in Chinese)
[9] 章丽娇 . 鸭坦布苏病毒细胞适应毒免疫原性及致弱分子基础研究[D]. 北京: 中国农业大学, 2017.
ZHANG L J . Immunogenicity and molecular basis for attenuation of BHK-21 cell-adapted duck tembusu virus[D]. Beijing: China Agricultural University, 2017. (in Chinese)
[10] 常超, 姜宇 . 鸡新城疫活疫苗血清学效力检验与靶动物免疫攻毒保护结果相关性试验. 饲料博览, 2018(4):36-37.
CHANG C, JIANG Y . Newcastle disease live vaccine serological efficacy test and the correlation test of the protective results of target animal immune attack. Feed Review, 2018, ( 4):36-37. (in Chinese)
[11] 潘杰, 秦涛, 范娟, 刘俊斌, 马若楠, 阴银燕 . 血清抗体检测代替攻毒保护法评估小鹅瘟灭活疫苗免疫效果的比较研究. 中国家禽, 2018,40(16):53-55.
PAN J, QIN T, FAN J, LIU J B, MA R N, YIN Y Y . A comparative study on the immune efficacy of inactivated gosling plague vaccine evaluated by serum antibody detection and by the challenging test. China Poultry, 2018,40(16):53-55. (in Chinese)
[12] 李新生, 彭志领, 李燕, 孟迪, 高文明, 崔保安 . 鸡新城疫、传染性支气管炎和禽流感(H9)联合免疫血清学效力与攻毒保护关系. 中国兽医学报, 2014,34(6):869-873.
LI X S, PENG Z L, LI Y, MENG D, GAO W M, CUI B A . The relationships of serological efficacy with the attack against newcastle disease virus, infectious bronchitis virus and avian influenza (H9) virus. Chinese Journal of Veterinary Science, 2014,34(6):869-873. (in Chinese)
[13] 藏玉婷, 张春媛 . 猪传染性胃肠炎、猪流行性腹泻二联灭活疫苗血清学效力检验与靶动物免疫攻毒保护相关性研究. 饲料博览, 2018(3):27-31.
ZANG Y T, ZHANG C Y . Correlation research of the antibody titer and virus attack protection of porcine transmissible gastroenteritis and porcine epidemic diarrhea inactivated vaccine. Feed Review, 2018(3):27-31. (in Chinese)
[14] 李慧姣, 蒋桃珍, 李启红, 谷红, 吴涛 . 鸡新城疫灭活疫苗免疫攻毒试验与血清学(HI)试验的平行关系研究. 中国兽药杂志, 2004(8):5-8.
LI H J, JIANG T Z, LI Q H, GU H, WU T . Studies on the relationship of challenge against newcastle disease and serology (HI) test. Chinese Journal of Veterinary Drug, 2004(8):5-8. (in Chinese)
[15] 王红琳, 汪宏才, 杨峻, 温国元, 罗青平, 罗玲, 艾地云, 张蓉蓉, 张腾飞, 邵华斌 . 禽霍乱、新城疫二联灭活疫苗免疫攻毒试验与HI试验的平行关系研究. 中国家禽, 2016,38(2):42-43.
WANG H L, WANG H C, YANG J, WEN G Y, LUO Q P, LUO L, AI D Y, ZHANG R R, ZHANG T F, SHAO H B . Parallel relationship between immune challenge test and HI test of avian cholera and Newcastle disease inactivated vaccine. China Poultry, 2016,38(2):42-43. (in Chinese)
[16] 白军, 刘淑红, 焦铁军, 王运宏, 黄建斌, 李河林, 马建玲, 赵希斌, 于三科 . 鸡新城疫、禽流感(H9亚型)二联油乳剂灭活疫苗免疫效力试验. 动物医学进展, 2013,34(3):58-61.
BAI J, LIU S H, JIAO T J, WANG Y H, HUANG J B, LI H L, MA J L, ZHAO X B, YU S K . Immune potency test of combined oil adjuvant inactivated vaccine against Newcastle disease and avian influenza (H9 Subtype). Progress in Veterinary Medicine, 2013,34(3):58-61. (in Chinese)
[17] 舒跃龙, 王敏, 张烨, 李梓, 郭俊峰, 郭元吉 . 一种敏感的H5禽流感病毒血凝抑制试验方法. 公共卫生与预防医学, 2007(2):9-10.
SHU Y L, WANG M, ZHANG Y, LI Z, GUO J F, GUO Y J . A more sensitive HI method for H5 avian influenza serum test. Journal of Public Health and Preventive Medicine, 2007(2):9-10. (in Chinese)
[18] 文英 . 青海省部分地区鸡新城疫与传染性支气管炎免疫抗体检测与分析[D]. 杨凌: 西北农林科技大学, 2009.
doi: 10.1016/j.ijfoodmicro.2019.108471 pmid: 31841786
WEN Y . Detection and analysis of chiken serum antibody levels against Newcastle disease and avian infectious bronchitis in partial area of Qinghai Province[D]. Yangling: Northwest A&F University, 2009. (in Chinese)
doi: 10.1016/j.ijfoodmicro.2019.108471 pmid: 31841786
[19] 中华人民共和国兽药典（三部）. 2015版. 北京: 中国农业出版社, 2016.
Veterinary Pharmacopoeia of the People's Republic of China （III）.2015. Beijing: China Agricultural Publishing House, 2016. (in Chinese)
[20] ZHANG W, CHEN S, MAHALINGAM S, WANG M, CHENG A . An updated review of avian-origin Tembusu virus: a newly emerging avian Flavivirus. Journal of General Virology, 2017,98(10):2413-2420.
doi: 10.1099/jgv.0.000908 pmid: 28874226
[21] LI X, LI G, TENG Q, YU L, WU X, LI Z . Development of a blocking ELISA for detection of serum neutralizing antibodies against newly emerged duck tembusu virus. PLoS ONE, 2012,7(12):83.
doi: 10.1371/journal.pone.0053026 pmid: 23300851
[22] ZHANG L, LI Z, JIN H, HU X, SU J . Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species. BMC Veterinary Research, 2018,14(1):201.
doi: 10.1186/s12917-018-1537-6 pmid: 29940964
[23] FU Y, JI Y, LIU B, DAFALLAH R M, ZHU Q . Development of a solid-phase competition ELISA to detect antibodies against newly emerged Duck Tembusu virus. Journal of Virological Methods, 2015,224:73-76.
doi: 10.1016/j.jviromet.2015.08.017 pmid: 26320577
[24] WANG Q, WEN Y, YIFAN H, WU Y, CAI Y, XU L, WANG C, LI A, WU B, CHEN J . Isolation and identification of duck Tembusu virus strain lH and development of latex-agglutination diagnostic method for rapid detection of antibodies. Avian Diseases, 2014,58(4):616-622.
[25] CASALS J, BROWN L V . Hemagglutination with arthropod-borne viruses. Journal of Experimental Medicine, 1954,99(5):429-449.
doi: 10.1084/jem.99.5.429 pmid: 13163320
[26] WANG X, YANG Z, WANG X, DUAN H, LIU L, CHENG H, YANG C, HOU L, PAN J, ZHAO J, LIU Y, LIN J . Development of a hemagglutination inhibition assay for duck tembusu virus.Avian Diseases, 2019(63):296-301.
doi: 10.1637/11954-082018-Reg.1 pmid: 31251530
[27] 林健, 杨志远, 何平有, 段会娟, 邹立宏, 杨保收, 赵际成, 潘洁, 王小蕾, 刘立新, 刘月焕 . 鸭坦布苏病毒病卵巢病变标准的判定. 中国农业科学, 2016,49(14):2830-2836.
doi: 10.3864/j.issn.0578-1752.2016.14.017
LIN J, YANG Z Y, HE P Y, DUAN H J, ZOU L H, YANG B S, ZHAO J C, PAN J, WANG X L, LIU L X, LIU Y H . Criterion of ovarian lesions of duck Tembusu virus disease. Scientia Agricultura Sinica, 2016,49(14):2830-2836. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2016.14.017
[28] 陈光华 . 实验动物与兽用生物制品检验和研发. 中国比较医学杂志, 2011,21(10/11):20-23.
CHEN G H . Experimental animals and quality control and development of veterinary biologicals. Chinese Journal of Comparative Medicine, 2011(10/11):20-23. (in Chinese)
[29] 王晓卉, 李翠, 蒋卉, 张秀英, 温芳, 陆连寿, 王在时 . 动物试验替代方法在兽用疫苗检验中的应用概况. 中国兽药杂志, 2015,49(4):60-64.
WANG X H, LI C, JIANG H, ZHANG X Y, WEN F, LU L S, WANG Z S . Application of alternatives to animal experiments in the evaluation of veterinary vaccine. Chinese Journal of Veterinary Drug, 2015,49(4):60-64. (in Chinese)
[30] 王兴童, 韩凌霞 . 兽用疫苗中替代方法的研究进展. 实验动物科学, 2018,35(1):67-71.
pmid: 3981961
WANG X T, HAN L X . Advance on non-animal alternative methods in animal vaccines. Laboratory Animal Science, 2018,35(1):67-71. (in Chinese)
pmid: 3981961
[31] 刘玉梅, 魏学峰, 陈九连, 黄凯, 苍枫, 董俊余, 张立华, 孙艳琪, 高换河, 王伟峰, 卢永干 . 牛口蹄疫Asia-Ⅰ型灭活疫苗LPB-E抗体滴度与免疫保护相关性研究. 中国兽药杂志, 2007(7):19-22.
LIU Y M, WEI X F, CHEN J L, HUANG K, CANG F, DONG J Y, ZHANG L H, SUN Y Q, GAO H H, WANG W F, LU Y G . Studies on the correlation between LPB-E antibody titer and immunoprotection rate of inactivated cattle foot-and-mouth disease Asia-Ⅰvaccine. Chinese Journal of Veterinary Drug, 2007(7):19-22. (in Chinese)
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