复方天然植物制剂对奶牛回乳效果及调控激素的影响

doi:10.3864/j.issn.0578-1752.2017.18.017

Abstract

Abstract: 【Objective】the objective of this study is to explore the impact of compound natural plant preparation on the milk withdrawal and serum insulin(INS), hydrocortisone(HC), growth hormone(GH) and insulin growth factor 1(TGF-1) levels in dairy cows.【Method】A total of 150 Chinese Holstein cows, weighing (582±41) kg, pregnant with 2-4 fetuses, and fed in a large-scale dairy farm in Sichuan Province from January 2016 to June 2016, were used in the present study. Eighty healthy cows with normal milk and udder that in late pregnancy and gave (15.42±0.71) kg milk per day were chosen. The cows were randomly divided into four groups: groups A, B, C and D, with 20 of them in each group. Stop dairy cows’ milk by gradually dry milk method and the day when cows gave less milk was recorded as 0 d. Group A was a control group, did not feed compound natural plant preparation and the groups B, C and D were fed 400g, 500g, 600g compound natural plant preparation, respectively, at 8.00 am from 1 d to the day of dry milk completely. Compound natural plant preparation comprises fructus hordei germinatus, mirabilite, rhizoma cimicifugae, bupleurum, rhizoma cyperi, coix seed, silkworm slough, rhizome atratylodis, scutellaria baicalensis, rhizome anemarrhenae, caulis perllae, semen euryales, schisandra chinensis, dandelion, licorice and so on. The cows’ venous blood was collected from tail on the day 0, 1, 3, 5, 7, 9 and 11, the samples were placed in the centrifuge tubes without anticoagulant at room temperature for 1h. The suspension was centrifuged at 352 × g for 10 min and then put into the refrigerator at -20℃. ELISA was used to evaluate the expression levels of serum INS, HC and IGF-I and the daily milk production was recorded.【Result】Groups A, B, C and D returned milk on the 11 d, 7 d, 5 d and 5 d respectively. The expression levels of serum IGF-1 and HC were declining in all 4 groups and the decline trend of the control group was slower than compound natural plant preparation groups. The expression levels of serum IGF-1, HC and GH were raised after the period of milk withdrawal, the upward trend of the expression levels of serum INS in the control group was slower than compound natural plant preparation groups. From the day 0 to the 1, the expression levels of serum HC, IGF-1 and GH had no significant difference (P＞0.05). From the day 3 to the day7, the expression levels of serum HC, GH and IGF-1 in group A were highly and significantly higher than those of groups B, C and D (P＜0.01), while the expression level of INS in group A was highly significant loser than those of groups B, C and D (P＜0.01). On the day 9, the expression levels of serum HC in groups A and D were significantly higher than that of group C (P＜0.05), the expression levels of serum GH and IGF-1 in groups A and B were highly and significantly lower than those of groups C and D (P＜0.01), the expression levels of serum INS in group A was highly and significantly lower than that of groups B, C and D (P＜0.01). On the day 11, the expression levels of serum HC, GH and IGF-1 in group A were highly significantly lower than those of groups B, C and D (P＜0.01), the expression levels of serum INS in group A was highly significant lower than that of groups B and C (P＜0.01) and significantly lower than that of group D (P＜0.05). The expression levels of serum IGF-1, GH, HC and INS had no significant difference between group C and D during the period of milk withdrawal (P＞0.05). During the period of milk withdrawal, there were significant positive correlations among milk production, HC, GH and IGF-1 (P＜0.01) and there was a significant negative correlation between INS and milk production, HC, GH, IGF-1 (P＜0.01).【Conclusion】 Compound natural plant preparation can help cows milk withdrawal significantly, feeding 500g·d^-1 can shorten the period of milk withdrawal to 5 days can make the best effect on milk withdrawal. The expression levels of serum INS, HC, IGF-1 and milk production had a highly significant correlation. IGF-1 GH and HC are negative regulation factors of milk withdrawal, while INS is a positive regulation factor.

Key words: dairy cow, milk withdrawal, compound natural plant preparation, galactin, dairy cow, milk withdrawal, compound natural plant preparation, galactin

SHEN LiuHong, XIAO JinBang, WU XiaoFeng, JIANG SiXun, JIANG Tao, DENG JunLiang, ZUO ZhiCai, YU ShuMin, CAO SuiZhong . Effects of Compound Natural Plant Preparation on Milk Withdrawal and Galactin in Dairy Cows[J].Scientia Agricultura Sinica, 2017, 50(18): 3620-3630.

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References

[1] 王春璈. 奶牛临床疾病学[M]. 北京：中国农业科学技术出版社, 2007.

WANG C A. Dairy Cow Clinical Disease[M]. Beijing: China Agricultural Science and Technology Press, 2007. (in Chinese)

[2] ZOBEL G, LESLIE K, WEARY D M, von KEYSERLINGK M A G. Gradual cessation of milking reduces milk leakage and motivation to be milked in dairy cows at dry-off. Journal of Dairy Science, 2013, 96(8): 5064-5071.

[3] 李嫔, 向正华, 蔡德培. 中药对大鼠下丘脑生长抑素及垂体生长激素基因表达与蛋白表达的调节作用. 中国中西医结合杂志, 2003, 23(3): 207-210.

LI B, XIANG Z H, CAI D P. Regulative effect of chinese herbal medicine on gene and protein expression levels of somatostatin and growth hormone in hypothalamus and hypophysis. Chinese Journal of Integrated Traditional and Western Medicine, 2003, 23(3): 207-210. (in Chinese)

[4] TUCKER C B, LACY-HULBERT S J, WEBSTER J R. Effect of milking frequency and feeding level before and after dry off on dairy cattle behavior and udder characteristics. Journal of Dairy Science, 2009, 92(7): 3194-3203.

[5] OGATA Y,YU G M, HIDAKA T, MATZUSHIGE T, MAEDA T. Effective embryo production from Holstein cows treated with gonadotropin-releasing hormone during early lactation. Theriogenology, 2016, 86(6): 1421-1426.

[6] 文静, 卜登攀, 王建发, 孙鹏. 激素调控乳蛋白合成的作用及其分子机制. 华北农学报, 2012, 27(s1): 111-115.

WEN J, BU D P, WANG J F, SUN P. Progress in the regulation role of lactoprotein synthesis by hormone and its molecular mechanism. Acta Agriculturae Boreali-Sinica, 2012, 27 (s1):111-115. (in Chinese)

[7] BACH L A. Insulin-like growth factor binding proteins--an update. Pediatric Endocrinology Reviews: Per, 2015, 13(2):521-30.

[8] 王璟. 中药对胰岛素治疗2型糖尿病低血糖发生率的影响. 西部中医药, 2014, 27(4): 94-95.

WANG J. Effects of herbs on the incidence rate of hypoglycemia of the patients with type two diabetes mellitus. Western Journal of Traditional Chinese Medicine, 2014, 27(4): 94-95. (in Chinese)

[9] 张秋华, 付彦君. 肥胖相关胰岛素抵抗中医药研究现状. 中医药信息, 2012, 29(2): 114-116.

ZHANG Q H, FU Y J. Obesity insulin resistance related research status of traditional Chinese medicine. Information on Traditional Chinese Medicine, 2012, 29(2): 114-116. (in Chinese)

[10] 王喜军, 张宁, 孙晖, 李丽静, 吕海涛. 六味地黄丸血中移行成分对氢化可的松致大鼠肾虚动物模型的保护作用研究. 中国实验方剂学杂志, 2008, 14(2): 33-37.

WANG X J, ZHANG N, SUN H, LI L J, LÜ H T. Protective effect of Liuwei Dihuang Wan and its constituents absorbed into blood after oral administration on hydrocortisone inducing renal deficiency in rat. Chinese Journal of Experimental Traditional Medical Formulae, 2008, 14(2): 33-37. (in Chinese)

[11] 苏成虎, 杨新波, 黄正明, 陈晓农, 陈红艳, 程丽娟, 于珊, 徐雪钰. 水芹提取物对氢化可的松致肾阳虚小鼠的对抗作用. 中国中医药信息杂志, 2011, 18(12): 39-42.

SU C H, YANG X B, HUANG Z M, CHEN X N, CHEN H Y, CHENG L J, YU S, XU X Y. Effect of extract of oenanthe javanica against kidney yang deficiency in mice caused by hydrocortisone. Chinese Journal of Information on Traditional Chinese Medicine, 2011, 18(12): 39-42. (in Chinese)

[12] 王金合, 李荣誉, 汪德刚, 王居强. 中草药添加剂对肉牛增重和血清中生长激素的影响. 河南农业科学, 2007 (9): 101-103.

WANG J H, LI R Y, WANG D, WANG J Q. The Chinese herb additive increases heavy and in the blood serum to the beef cattle the growth hormone influence. Journal of Henan Agricultural Sciences, 2007(9): 101-103. (in Chinese)

[13] 石宝明, 单安山. 促乳中草药对泌乳大鼠内分泌功能的影响. 东北农业大学学报, 2011, 42(12): 45-50.

SHI B M, SHAN A S. Effect of Chinese herbs for improving milk production on dam endocrine function during suckling period. Journal of Northeast Agricultural University, 2011, 42(12): 45-50. (in Chinese)

[14] 王雄. 回乳抑增一号治疗高泌乳素血症与乳腺增生药效学研究[D]. 武汉: 湖北中医药大学, 2012.

WANG X. Pharmacodynamics study on menehanism of NO.1 Huiru Yizeng on hyperprolactinemia and mazoplasia in rats[D]. Wuhan: Hubei University of Chinese Medicine, 2012. (in Chinese)

[15] 郝振华, 郭震兵. 重用生麦芽治疗高泌乳素血症性不孕的临床观察. 新疆中医药, 2016, 34(1): 13-14.

HAO Z H,GUO Z B. The clinical observation of treating infertility with high prolactin with large-dose raw malt. Xinjiang Journal of Traditional Chinese Medicine, 2016, 34(1): 13-14. (in Chinese)

[16] 钟慧萍. 炒麦芽泡茶饮用联合芒硝外敷双乳回奶的效果观察. 中外医学研究, 2015,13(34): 157-159.

ZHONG H P. Fried malt tea drinking joint of glauber's salt topical breasts back to observe the effect of milk. Chinese and Foreign Medical Research, 2015, 13(34): 157-159. (in Chinese)

[17] MACRINA A L, KAUF A C W, KENSINGER R S. Effect of bovine somatotropin administration during induction of lactation in 15- month-old heifers on production and health. Journal of Dairy Science, 2011, 94(9): 4566-4573.

[18] WANG J F, FU S P, LI S N, HU Z M, XUE W J, LI Z Q, HUANG B X, LV Q K, LIU J X, WANG W. Short-chain fatty acids inhibit growth hormone and prolactin gene transcription via cAmp/PKA/CREB signaling pathway in dairy cow anterior pituitary cells. International Journal of Molecular Sciences, 2013, 14(11): 21474-21488.

[19] HENDERSON A C, HUDSON C D, BRADLEY A J, SHERWIN V E, GREEN M J. Prediction of intramammary infection status across the dry period from lifetime cow records. Journal of Dairy Science, 2016, 99(7): 5586-5595.

[20] ZHAO F R, MAO H P,ZHANG H, HU L M, WANG H, WANG Y F, YANAQIHARA N, GAO X M. Antagonistic effects of two herbs in Zuojin Wan, a traditional Chinese medicine formula, on catecholamine secretion in bovine adrenal medullary cells. Phytomedicine International Journal of Phytotherapy and Phytopharmacology, 2010, 17(8-9): 659-668.

[21] 吴文英, 周惠欢, 吴秀娥. 芒硝外敷治疗产后回奶的观察及护理. 医学信息, 2013, 26(8):375.

WU W Y, ZHOU H H, WU X E. Glauber's salt external observation and nursing for the treatment of postpartum back to milk. Medical Information, 2013, 26(8):375. (in Chinese)

[22] 杨强, 陈晋, 刘军. 卡麦角林治疗泌乳素型垂体瘤的研究进展. 中国神经精神疾病杂志, 2016, 42(5):311-314.

YANG Q, CHEN J, LIU J. Cabergoline therapy research progress of prolactin type of pituitary adenoma. Chinese Journal of Nervous and Mental Diseases, 2016, 42(5): 311- 314. (in Chinese)

[23] RIEHLE C, ABEL E D. Insulin signaling and heart failure. Circulation Research, 2016,118(7):1151- 1169.

[24] AKERS R M. Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. Journal of Dairy Science, 2006, 89(4): 1222-1234.

[25] 佟慧丽, 高学军, 李庆章, 严云勤. 胰岛素、催乳素对奶山羊乳腺上皮细胞泌乳功能的影响. 畜牧兽医学报, 2008, 39(6): 721-725.

TONG H L, GAO X J, LI Q Z, YAN Y Q. Impacting of insulin and prolactin on mammary gland epithelial cell line. Chinese Journal of Animal and Veterinary Sciences, 2008, 39(6): 721-725. (in Chinese)

[26] 陈建晖, 佟慧丽, 李庆章, 高学军. 胰岛素、催乳素和孕酮对奶牛乳腺上皮细胞泌乳功能的影响. 中国奶牛, 2008(8): 9-13.

CHEN J H, TONG H L, LI Q Z, GAO X J. Influence of insulin, prolactin and progesterone on milk-secr etion of mammary gland epithelial cell in dairy cow. China Dairy, 2008(8):9-13. (in Chinese)

[27] 田青, 季昀, 庞学燕, 王洪荣. 胰岛素对奶牛乳腺上皮细胞酪蛋白合成调节机理的研究. 动物营养学报, 2013, 25(3): 550-560.

TIAN Q, JI Y, PENG X Y, WANG H R. A study of insulin action mechanism on casein synthesis of bovine mammary epithelial cells. Chinese Journal of Animal Nutrition, 2013, 25(3): 550-560. (in Chinese)

[28] PANG B, YU X T, ZHOU Q, ZHAO T Y, WANG H, GU C J, TONG X L. Effect of Rhizoma coptidis (Huang Lian) on treating diabetes mellitus. Evidence-based Complementary and Alternative Medicine, 2015:921416. http://dx.doi.org/10.1155/2015/921416.

[29] 毕博, 孙兰军. 中药改善胰岛素抵抗及其降糖作用. 现代中西医结合杂志, 2009, 18(22): 2739-2740.

BI B, SUN L J. Traditional Chinese medicine to improve insulin resistance and its hypoglycemic effect. Modern Journal of Integrated Traditional Chinese and Western Medicine, 2009, 18(22): 2739-2740. (in Chinese)

[30] 李敏. 新型糖皮质激素对肥大细胞脱颗粒的快速作用及其机制研究[D]. 第二军医大学, 2010.

LI M. New glucocorticoid on mast cell degranulation quick effect and its mechanism[D]. Second Military Medical University, 2010. (in Chinese)

[31] SHEEHY P A, NICHOLAS K R, WYNN P C. An investigation of the role of insulin in bovine milk protein gene expression in mammary explant culture. Asian Australasian Journal of Animal Sciences, 2000, 13:272-275.

[32] KABOTYANSKI E B, RIJINKELS M, FREEMAN-ZADROWSKI C, BUSER A C, EDWARDS D P, ROSEN J M. Lactogenic hormonal induction of long distance interactions between Β-Casein gene regulatory elements. Journal of Biological Chemistry, 2009, 284(34): 22815-22824.

[33] LITTLEJOHN M D, HENTY K M, TIPLADY K, JOHNSON T, HARLAND C, LOPDELL T, SHERLOCK R G, LI W, LUKEFAHR S D, SHANKS B C, GARRICK D J, SNELL R G, SPELMAN R J, DAVIS S R. Functionally reciprocal mutations of the prolactin signalling pathway define hairy and slick cattle. Nature Communications, 2014, 5: 5861-5861.

[34] WHITAKER M J, SPIELMANN S, DIGWEED D, HUATAN H, ECKLAND D, JOHNSON T N, TUCKER G, KRUDE H, BLANKENSTEIN O, ROSS R J. Development and testing in healthy adults of oral hydrocortisone granules with taste masking for the treatment of neonates and infants with adrenal insufficiency. Journal of Clinical Endocrinology & Metabolism, 2015, 100(4): 1681-1688.

[35] STEYN F J. Nutrient sensing ooverrides somatostatin and growth hormone‐releasing hormone to control pulsatile growth hormone release. Journal of Neuroendocrinology, 2015, 27(7): 577-587.

[36] BROWN-BORG H M. Reduced growth hormone signaling and methionine restriction: interventions that improve metabolic health and extend life span. Annals of the New York Academy of Sciences, 2015, 1363(1): 40-49.

[37] FORSYTH I A, WALLIS M. Growth hormone and prolactin— molecular and functional evolution. Journal of Mammary Gland Biology and Neoplasia, 2002, 7(3): 291-312.

[38] RHOADS M L, MEYER J P, KOLATH S J, LAMBERSON M C, LUCY M C. Growth hormone receptor, insulin-like growth factor (IGF)-1, and IGF-binding protein-2 expression in the reproductive tissues of early postpartum dairy cows. Journal of Dairy Science, 2008, 91(5): 1802-1813.

[39] ELENA L, BERIT K, BJORN J, KERSTIN A W. Growth hormone (GH) dose-dependent IGF-I response relates to pubertal height gain. Bmc Endocrine Disorders, 2015, 15(1): 1-14.

[40] LO Y H, CHEN Y J, CHUNG T Y, LIN N H, CHEN W Y, CHEN C Y, LEE M R, CHOU C C, TZEN-JASON T C. Emoghrelin, a unique emodin derivative in Heshouwu, stimulates growth hormone secretion via activation of the ghrelin receptor. Journal of Ethnopharmacology, 2015, 159(5): 1-8.

[41] NAWATHE A R, CHRISTIAN M, KIM S H, JOHSON M, SAVVIDOU M D, TERZIDOU V. Insulin-like growth factor axis in pregnancies affected by fetal growth disorders. Clinical Epigenetics, 2016, 8(1): 1-13.

[42] MURNEY R, STELWAGEN K, WHEELER T T, MARGERISON J K, SINGH K. The effects of milking frequency on insulin-like growth factor 1 signaling within the mammary gland of dairy cows. Journal of Dairy Science, 2015, 98(8): 5422-5428.

[43] HERNANDEZ H, FLORES J A, DELGADILLO J A, FERNANDEZ I G, FLORES M J, MEJIA A, ELIZUNDIA J M, BEDOS M, PONCE J L, RAMIREZ S. Effects of exposure to artificial long days on milk yield, maternal insulin-like growth factor 1 levels and kid growth rate in subtropical goats. Animal Science Journal, 2016, 87(4):484-491.

[44] 王昶. 胰岛素样生长因子-1在肝纤维化大鼠中的表达及中药的干预作用[D]. 大连：大连医科大学, 2015.

WANG C. Insulin-like growth factor 1 expression in liver fibrosis in rats and the intervention effect of Chinese traditional medicine[D]. Dalian：Dalian Medical University, 2015. (in Chinese)

[45] 唐中生, 吕明庄, 贺志光, 王旭东. 耳针联合中药对血管性痴呆大鼠记忆障碍及胰岛素样生长因子-1及乙酰胆碱酯酶表达的影响. 中华中医药杂志, 2008, 23(3): 270-272.

TANG Z S, LÜ M Z, HE Z G, WANG X D. Earpins combined traditional Chinese medicine (TCM) memory impairment of vascular dementia rats and insulin-like growth factor 1 and acetyl cholinesterase expression. China Journal of Traditional Chinese Medicine and Pharmacy, 2008, 23(3): 270-272. (in Chinese)

[46] 张弛, 杨志伟, 段小英. 活血通络法治疗激素性股骨头坏死兔胰岛素样生长因子1的变化. 中国组织工程研究, 2012, 16(52): 9787-9791.

ZHANG C, YANG Z W, DUAN X Y. Changes of insulin-like growth factor 1 levels in rabbits with steroid-induced femoral head necrosis after treatment with Huoxuetongluo method. Chinese Journal of Tissue Engineering Research, 2012, 16(52): 9787-9791. (in Chinese)

[47] 李蔚辉, 魏学鑫. 糖皮质激素的信号转导系统. 亚太传统医药, 2007, 3(12): 21-25.

LI W H, WEI X X. Signal transduction system of glucocorticoid. Asia-Pacific Traditional Medicine, 2007, 3(12): 21-25. (in Chinese)

[48] 赵国丽, 宫艳斌, 韩元, 李鹏, 秦春华, 史远刚. 激素和生长因子调控奶牛乳腺发育的研究进展. 中国奶牛, 2011(6): 25-30.

ZHAO G L, GONG Y B, HAN Y, LI P, QIN C H, SHI Y G. Advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. China Dairy, 2011(6): 25-30. (in Chinese)

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