热应激状态下泌乳奶牛通过激活GHIGF-I轴增强糖异生变化

doi:10.3864/j.issn.0578-1752.2016.15.018

Abstract

Abstract: 【Objective】Six Holstein cows in early lactation after delivery were chosen to be in heat stress,which were fed 35 days from June 29 in 2013 for detecting milk yield and protein content, growth hormone (GH), insulin like growth factor-I(IGF-I), glucose and liver heat shock protein 70 (HSP70) and gluconeogenesis change in blood to illuminate glucose gluconeogenesis and milk quality decline mechanism when lactating dairy in heat stress from the GHIGF-I axis angle and further revealing the cows heat stress mechanism and control of dairy cows heat stress.【Method】Glucose and GH, and IGF-I contents in blood were detected by collecting jugular venous blood and in vivo taking liver tissue, furthermore milk yield and protein content in lactating cows were counted and analyzed, respectively, before fifth week. In addition, HSP70, PC, PEPCK, GHR, and IGFR were detected by qRT-PCR.【Result】Dairy cows were in heat stress when the average daytime temperature above 32℃ lasted for 25 days and the highest temperature 38℃ lasted for more than 72 h continuously during 35 days feeding period. The milk yield and protein content generally showed a decline with the degree of heat stress in lactating dairy cows deepening from the first week to the fifth week. The expression of HSP70 in the fifth week was significantly higher than that in the first week, so were the expression of PC and PEPCK and GHR and IGFR in liver tissues (P＜0.05) through detecting their contents.【Conclusion】Blood glucose was significantly increased, in the period of study which may be due to the secretion of pituitary GH stimulates the liver to produce more IGF-I, namely through GHIGF-I axis up-regulation of hepatic gluconeogenesis pathway key enzyme expression, gluconeogenesis way was activated with the degree of heat stress in lactating dairy cows deepening. Milk protein content decline may be caused by its precursors was too much for gluconeogenesis to make levels of glucose increase in blood to maintain body’s normal energy supply.

Key words: lactaing dairy cows, heat stress, GHIGF-I axis, growth hormones (GH), gluconeogenesis

LI Lin, AI Yang, XIE Zheng-lu, CAO Yang, ZHANG Yuan-shu. Lactating Dairy Cows Under Heat Stress Enhanced Gluconeogenesis by Activating the GHIGF-I Axis[J].Scientia Agricultura Sinica, 2016, 49(15): 3046-3053.

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Lactating Dairy Cows Under Heat Stress Enhanced Gluconeogenesis by Activating the GHIGF-I Axis

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