Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (3): 556-561.doi: 10.3864/j.issn.0578-1752.2012.03.018

• VETERINARY SCIENCE • Previous Articles     Next Articles

Expression of Nod-2 Receptor in Periparturient Dairy Cows and Its Effect on Neutrophils Respiratory Burst

 LI  Wen-Wen, LU  Zheng, TAN  Xun, LI  Jun-Jun   

  1. 1.浙江大学动物科学学院/农业部动物病毒学重点实验室，杭州 310058
  • Received:2011-06-20 Online:2012-02-01 Published:2011-11-10

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Abstract: 【Objective】 Dairy cows are more susceptible to bacterial diseases around parturition. The present study was conducted to investigate the expression of Nod-2 in the polymorphonuclear neutrophil leukocytes (PMN) from periparturient cows and its effect on PMN respiratory burst, with the purpose to evaluate whether the Nod-2 receptor contributes to the increased susceptibility of periparturient cows to bacterial diseases. 【Method】 The Nod-2 mRNA in PMN from periparturient and non- periparturient cows was detected by RT-PCR. Function of respiratory burst in PMNs was evaluated by flow cytometry following the stimulation of the whole blood with phorbol-12-myristate-13-acetate (PMA) and the ligand of Nod-2, muramyl dipeptide (MDP), respectively. 【Result】 It was found that the blood PMN from periparturient cows had decreased Nod-2 mRNA expression than that from non-periparturient lactating cows (P＜0.05), and their ability of respiratory burst responding to PMA stimulation was decreased (P＜0.05). Upon the MDP stimulation, PMNs from periparturient cows showed decreased respiratory burst as well. 【Conclusion】 It was suggested that the reduced Nod-2 mRNA expression might contribute to the increased susceptibility to bacterial infection in periparturient cows.

Key words: dairy cow, neutrophil, Nod-2 receptor

[1]Spears J W, Weiss W P. Role of antioxidants and trace elements in health and immunity of transition dairy cows. The Veterinary Journal, 2008, 176:70-76.

[2]Le Blanc S J. Postpartum uterine disease and dairy herd reproductive performance: a review. The Veterinary Journal, 2008, 176:102-114.

[3]Kumar V, Sharma A. Neutrophils: cinderella of innate immune system. International Immunopharmacology, 2010, 10:1325-1334.

[4]Jall M, Hilde D, Evelyne M, Roger H, Christian B. Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation. Journal of Dairy Research, 2001, 68:399-415.

[5]Franchi L, Warner N, Viani K, Nunez G. Function of Nod-like receptors in microbial recognition and host defense. Immunological Review, 2009, 227:106-128.

[6]McDonald C, Inohara N, Nunez G. Peptidoglycan signaling in innate immunity and inflammatory disease. Journal of Biological Chemistry, 2005, 280:20177-20180.

[7]Franchi L, Park J H, Shaw M H, Marina-Garcia N, Chen G, Kim Y G, Nunez G. Intracellular NOD-like receptors in innate immunity, infection and disease. Cellular Microbiology, 2008, 10:1-8.

[8]Inohara N, Ogura Y, Fontalba A, Gutierrez O, Pons F, Crespo J, Fukase K, Inamura S, Kusumoto S, Hashimoto M, Foster S J, Moran AP, Fernandez-Luna J L, Nunez G. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn’s disease. Journal of Biological Chemistry, 2003, 278:5509-5512.

[9]Hruz P, Zinkernagel A S, Jenikova G, Botwina G J, Hugotc J P, Karind M, Nizetb V, Eckmanna L. NOD2 contributes to cutaneous defense against Staphylococcus aureus through α-toxin-dependent innate immune activation. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106:12873-12878.

[10]Conejeros I, Jara E, Carretta M D, Alarcon P, Hidalgo M A, Burgos R A. 2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11b expression, and increases L-selectin shedding in bovine neutrophils. Research in Veterinary Science, 2012, 92: 103-110.

[11]Chen G, Shaw M H, Kim Y G Nunez G. NOD-like receptors: role in innate immunity and inflammatory disease. Annual Review of Pathology, 2009, 4:365-398.

[12]Magalhaes J G, Lee J, Geddes K, Rubino S, Philpott D, Girardin S E. Essential role of Rip2 in the modulation of innate and adaptive immunity triggered by Nod1 and Nod2 ligands. European Journal of Immunology, 2011, 41:1445-1455.

[13]Kawai T, Akira S. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity, 2011, 34:637-650.

[14]Tada H, Aiba S, Shibata K, Ohteki T, Takada H. Synergistic effect of Nod1 and Nod2 agonists with toll-like receptor agonists on human dendritic cells to generate interleukin-12 and T helper type 1 cells. Infection and Immunity, 2005, 73:7967-7976.

[15]Takahashi Y, Isuzugawa K, Murase Y, Imai M, Yamamoto S, Iizuka M, Akira S, Bahr G M, Momotani E, Hori M, Ozaki H, Imakawa K. Up-regulation of NOD1 and NOD2 through TLR4 and TNF-alpha in LPS-treated murine macrophages. Journal of Veterinary Medicine Science, 2006, 68:471-478.

[16]Kim Y G, Park J H, Shaw M H, Franchi L, Inohara N, Nunez G. The cytosolic sensors Nod1 and Nod2 are critical for bacterial recognition and host defense after exposure to Toll-like receptor ligands. Immunity, 2008, 28:246-257.

[17]Kapetanovic R, Nahori M A, Balloy, Fitting C, Philpott D J, Cavaillon J M, Conquy A M. Contribution of phagocytosis and intracellular sensing for cytokine production by Staphylococcus aureus-activated macrophages. Infection and Immunity, 2007, 75:830-837.

[18]Ekman A K, Cardell L O. The expression and function of Nod-like receptors in neutrophils. Immunology, 2010, 130:55-63.

[19]Frutuoso M S, Hori J I, Pereira M S, Juniora D S, Sonegob F, Kobayashic K S, Flavelld R A, Cunhab F Q, Zamboni D S. The pattern recognition receptors Nod1 and Nod2 account for neutrophil recruitment to the lungs of mice infected with Legionella pneumophila. Microbes and Infection, 2010, 12:819-827.

[20]Burvenich C, Bannerman D D, Lippolis J D, Peelman L, Nonnecke B J, Kehrli M E Jr, Paape M J. Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period. Journal of Dairy Scinence, 2007, 90(Suppl. 1):E39-54.

[21]Perkins K H, VandeHaar M J, Tempelman R J, Burton J L. Negative energy balance does not decrease expression of leukocyte adhesion or antigen-presenting molecules in cattle. Journal of Dairy Science, 2001, 84:421-428.

[22]Meglia G E, Johannisson A, Agenas S, Holtenius K, Waller K P. Effects of feeding intensity during the dry period on leukocyte and lymphocyte sub-populations, neutrophil function and health in periparturient dairy cows. The Veterinary Journal, 2005, 169: 376-384.

[23]Goff J P, Horst R L. Physiological changes at parturition and their relationship to metabolic disorders. Journal of Dairy Science, 1997, 80: 1260-1268.

[24]Burton J L, Madsen S A, Chang L C, Weber P S, Buckham K R, van Dorp R, Hickey M C, Earley B. Gene expression signatures in neutrophils exposed to glucocorticoids: A new paradigm to help explain “neutrophil dysfunction” in parturient dairy cows. Veterinary Immunology and Immunopathology, 2005, 105:197-219.

[25]Weber P S, Madsen-Bouterse S A, Rosa G J, Sipkovsky S, Ren X, Almeida P E, Kruska R, Halgren R G, Barrick J L, Burton J L. Analysis of the bovine neutrophil transcriptome during glucocorticoid treatment. Physiological Genomics, 2006, 28:97-112.

[26]Sabroe I, Prince L R, Jones E C, Horsburgh M J, Foster S J, Vogel S N, Dower S K, Whyte M K. Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span. Journal of Immunology, 2003, 170:5268-5275.

[27]Conejeros I, Patterson R, Burgos R A, Hermosilla C, Werling D. Induction of reactive oxygen species in bovine neutrophils is CD11b, but not dectin-1-dependent. Veterinary Immunology and Immunopathology, 2011, 139:308-312.

[28]Babior B M. NADPH oxidase: an update. Blood, 1999, 93:1464-1476.

[29]Franchi L, Park J H, Shaw M H, Marina-Garcia N, Chen G, Kim Y G, Nunez G. Intracellular NOD-like receptors in innate immunity, infection and disease. Cellular Microbiology, 2008, 10:1-8.

[30]Hakkim A, Fuchs T A, Martinez N E, Hess S, Prinz H A, Waldmann H. Activation of the RAF-MEK-ERK pathway is required for neutrophil extracellular trap formation. Nature Chemical Biology, 2011, 7:75-77.

[31]Bercot B, Kannengiesser C, Oudin C, Grandchamp B, Pors MS, Mouly S, Elbim C. First description of NOD2 variant associated with defective neutrophil responses in a woman with granulomatous mastitis related to corynebacteria. Journal of Clinical Microbiology, 2009, 47:3034-3037.
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