Abstract: 【Objective】The objective of this experiment is to study comprehend the effect of cefquinome sulfate uterus infusion on the normal body temperature, production performance and bacteria in the uterus of dairy cows. 【Method】Ten healthy dairy cows were selected in this experiment. The selected cows were fed normally and not given any antibiotics systemicly or in uterus within 30 days before the test. There were not any antimicrobial drugs in daily ration and drinking water of cows. The total daily milk yields of all experimental cows were recorded 1 day before administration (early, middle and late yield). On the day of administration, the rectal temperature was measured and recorded, then the uterus secretion was collected and detected. The edge and inside of the vagina were disinfected with 75% alcohol before collection, then uterus secretion was received by sterilization of Uterus Syringe for Animal Use. One piece of cefquinome sulfate uterus infusion (dose:25ml:900mg/piece) was injected into uterus of each cow for 2 times，and its interval of the administration was 72 h. During the administration and sampling period, the cows were treated normally. Daily milk yields and rectal temperature of all experimental cows were recorded, respectively, on day 1, 3, 5, 7 and 10 after the last administration. Uterus secretion was collected and detected on the day10 after the last administration. The differences of daily milk yield，rectal temperature and the changes of pathogen in uterus secretion before and after administration were compared and analyzed.【Result】The mean daily milk yield of experimental cows of 1 day before administration and day1, 3, 5, 7 and10 after the last administration were 28.8, 29.2, 29.7, 29.6, 29.9 and 30.2 kg, respectively. There was a certain increase overall, but there were some fluctuations during the period. The X2 test results showed that there was no significant difference of daily milk yield between each time point (P＞0.05). At the same time, the mean value of rectal temperature of each time point was 38.5, 38.5, 38.4, 38.4, 38.4 and 38.4℃, respectively, and there was also no significant difference between each time point (P＞0.05). The results showed that when cows were administrated recommended dosages of cefquinome sulfate uterus infusion, there were no significant differences in indicators all above before or after the administration (P＞0.05). Pathogen detection results showed that the number of Escherichia coli, Streptothrix, Staphylococcus and Corynebacterium pyogenes were 3, 3, 2 and 1 before administration and 2, 0, 1 and 0 after administration, respectively. The number of pathogenic bacteria in the uterus secretion decreased after administration. At the same time, new pathogens were not detected. 【Conclusion】Cefquinome sulfate uterus infusion had no harmful effect on the normal body temperature, production performance and bacteria in the uterus of dairy cows, which indicated that cefquinome sulfate uterus infusion is safe for dairy cows when administrated according to the recommended dosing regime.

Key words: cefquinome sulfate, uterus infusion, dairy cows, safety