Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4688-4696.doi: 10.3864/j.issn.0578-1752.2012.22.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Construction and Identification of Normalized Subtractive cDNA Library of Plumage Color-Related Genes in Mule Duck

 ZHENG  Nen-Zhu, CHEN  Xiao-Yan, LU  Li-Zhi, ZHU  Zhi-Ming, MIAO  Zhong-Wei, XIN  Qing-Wu, CHEN  Hui, XIAO  Tian-Fang   

  1. 1.Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013
    2.Institute of Animal Science and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021
    3.Institute of Animal Science, Fujian Agricultural and Forestry University, Fuzhou 350002
  • Received:2012-04-19 Online:2012-11-15 Published:2012-08-27

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Abstract: 【Objective】 The aim of this study was to isolate plumage color related-genes in mule duck and explore its molecular mechanism. 【Method】 A normalized subtractive library of plumage color related-genes of mule duck was constructed. White plumage skin and black plumage skin were adopted respectively as the tester and driver, and a duplex-specific nuclease (DSN) normalized subtractive hybridization method was used to construct the library, and real-time PCR was employed to identify the quality of the library.【Result】Results showed that 64 genes with the average size of 1 031 bp were obtained by selecting and sequencing 144 positive clones randomly. Nucleotide BLAST homological analysis incidated that 21 genes had similarities to known genes which enjoy 92.8% homology, and 43 genes were not matched which presumably may be new genes related to plumage color. Geneontology displayed that these known genes were involved in many biological processes such as signal transduction, cell structure, material transport, apoptosis, cell and organism defense, transcription and expression regulation，and had different correlations with formation and transshipment of pigment. 【Conclusion】 It was determined that the library had good quality and could enrich the genes of the white plumage via real-time PCR.

Key words: mule duck , plumage color gene , duplex-specific nuclease (DSN) , subtractive hybridization , real-time PCR

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