鸭梨,PbChiIV,表达模式,SA,梨轮纹病菌," /> 鸭梨,PbChiIV,表达模式,SA,梨轮纹病菌,"/> Cloning and Expression of Chitinase <em>PbChiIV</em> Genes from <em>Pyrus bretschneideri</em> ‘Yali’

Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (11): 2279-2286.doi: 10.3864/j.issn.0578-1752.2015.11.019

• RESEARCH NOTES • Previous Articles    

Cloning and Expression of Chitinase PbChiIV Genes from Pyrus bretschneideri ‘Yali’

LI Peng-peng1, LIU Ning2, ZHANG Yu-xing1, PENG Jian-ying1, DONG Jin-gao2   

  1. 1College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei
    2College of Life Science, Agricultural University of Hebei/The Lab of Mycotoxin & Molecular Plant Pathology, Baoding 071001, Hebei
  • Received:2014-10-19 Online:2015-06-01 Published:2015-06-01

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Abstract: 【Objective】This study aimed to clone a full length cDNA of PbChiIV，fruit and the expression after treatment with the salicylic acid(SA) and Botryosphaeria berengeriana de. Not. f. sp. piricola (Nose) Koganecawa et. Sokwwa.【Method】The full lengthof PbChiIV was obtained from Pyrus bretschneideri ‘Yali’ using specific primers. The similarity of the nucleotide sequence and deduced amino acid sequence was analyzed by using BLAST on NCBI. BiotEdit software was used to compare the amino acid sequence. Phylogenetic tree was constructed by using MEGA6.0. Quantitative real-time PCR (qRT-PCR) was performed to determine the expression pattern of PbChiIV in different tissues and the expression of this gene after treatment with SA and B.berengeriana. f. sp. piricola.【Result】The cDNA lengths of PbChiIV genes was 819 bp (GenBank accession number: KJ872676), encoding 272 amino acids. The deduced amino acid sequence shares 100%, 79%, 73%, 73%, 72%, 72%, 72%, 69%, 68%, 67%, 67%, 65%, 67% and 62% identity with P. pyrifolia ACM45716.1, Populus trichocarpa XP_006376418.1, Vitis vinifera NP_001268173.1, Arabidopsis thaliana CAA74930.1, Medicago sativa ACL36992.1, Medicago truncatula AAR87869.1, Vigna unguiculata CAA61281.1, Corylus heterophylla AEM97876.1, Galega orientalis AAP03085.1, Vitis vinifera NP_001268075.1, Vitis pseudoreticulata ABY66958.1, Vitis vinifera AAB65777.1, Nicotiana tabacum BAF44533.1 and Gossypium barbadense AER29902.1. This gene belongs to IV chitinase gene. Expression analysis showed that the expression of PbChiIV in root was the highest, which was 4.32 and 2.96 times compared with stem and leaf, respectively. Followed by the expression in fruit, which was 2.48 and 1.70 times compared to that of stem and leaf, respectively. In the young fruit and mature fruit, the expression of the gene could be induced by SA and B.berengeriana. f. sp. piricola. The highest expression of the gene was 2.83 and 3.80 times compared with controls after treatment with SA, respectively. The highest expression of the gene was 1.82 and 1.66 times as compared with controls after treatment with pathogen, respectively. The highest expression of the gene was 2.49 and 3.43 times as compared with controls after treatment with SA and pathogen. In addition, the expression was up to the peak at 72, 24 and 72 h after treatment with SA and pathogen, respectively.【Conclusion】PbChiIV may be involved in SA-mediated basic signaling pathway of plant defense responses and disease resistance signal transduction network. It is speculated that PbChiIV is involved in defense responses mediated by B.berengeriana. f. sp. piricola. It might play an important role in disease resistance. from Pyrus bretschneideri ‘Yali’ and detect the expression characteristics of PbChiIV in root, stem, leaf

Key words: Pyrus bretschneideri ‘Yali’, PbChiIV de. Not. f. sp. piricola (Nose) Koganecawa et. Sokwwa), expression pattern, SA, pear ring rot pathogen (Botryosphaeria berengeriana

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