腐胺和脯氨酸对哺乳期仔猪空肠绒毛-隐窝轴上皮细胞的多胺代谢及Wnt信号通路的影响

doi:10.3864/j.issn.0578-1752.2015.08.15

Abstract

Abstract: 【Objective】The aim of this paper is to study the effects of putrescine and proline on polyamines (putrescine, spermidine and spermine) metabolites, relative mRNA expression levels of Wnt signaling molecules along the villus-crypt axis in the jejunum of sucking piglet. 【Method】 Eighteen 0-day old newborn crossbred (Landrace × Yorkshire × Duroc) piglets were randomly divided into three groups: control group, putrescine group and proline group. The piglets in control group were administered an equal volume of saline, and that in other two groups received putrescine (5 mg·kg^-1 body weight ) and proline (25 mg·kg^-1 body weight ), respectively. Piglets were weaned at 14-day old and slaughtered on 3 days postweaning. The jejuna epithelial cells along the crypt-villus axis were isolated by Krebs Henseleit (KH) solution and yield 3 “cell fractions” (Fraction 1, 2 and 3). The concentration of polyamine in F1, F2 and F3 was determined by high performance liquid chromatography, and relative mRNA expression of related genes of polyamine metabolic and wnt signaling pathways were measured by real time PCR. 【Result】 The concentration of putrescine, spermine and spermidine in F1 were significantly increased (P＜0.05) in proline group compared to control group, while there was no significant difference in putrescine group (P＞0.05). The concentrations of spermine and spermidine in F2 of proline or putrescine groups were significantly higher than that of control group (P＜0.05). the concentrations of putrescine, spermine and spermidine in F3 of three group were not changed significantly (P＞0.05). The expression of ornithine decarboxylase (ODC) gene in F1 of putrescine group was significantly higher than that of proline group and control group (P＜0.05). The expression of arginase in F2 of proline group was significantly higher than that of putrescine group and control group (P＜0.05). The expression of SFRP3 in F2 of proline group was significantly higher than that of putrescine group (P＜0.05); the expression of sFRP4 in F3 of putrescine group is significantly higher than that of control group and proline group (P＜0.05). 【Conclusion】 Exogenous putrescine and proline increased the concentration of polyamine in differentiated epithelial cells of jejunum in sucking piglet and promoted the polyamine metabolism, however, no statistically significant effect was observed on crypt cell. In addition, exogenous putrescine and proline promote differentiation in the intestinal epithelial cells by Wnt signaling pathways.

Key words: putrescine, proline, jejunum, polyamine, Wnt

WANG Xiao-cheng, XIONG Xia, YANG Huan-sheng, GAO Wei, GONG Min, YIN Yu-long. Impact of Putrescine and Proline on Suckling Piglet Jejunum Villus - crypt Axis Epithelial Polyamine Metabolism and Wnt Signal Pathway[J].Scientia Agricultura Sinica, 2015, 48(8): 1609-1615.

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References

[1] Svensson F, Persson L. Regulation of ornithine decarboxylase and S-adenosylmethionine decarboxylase in a polyamine auxotrophic cell line. Molecular and Cellular Biochemistry, 1996, 162(2): 113-121.

[2] Vellenga L, Wensing T, Egberts H J, van Dijk J E, Mouwen J M, Breukink H J. Intestinal permeability to macromolecules in piglets infected with transmissible gastroenteritis virus. Veterinary Research Communications, 1988, 12(6): 481-489.

[3] Russo F, Linsalata M, Giorgio I, Caruso M L, Armentano R, Di Leo A. Polyamine levels and ODC activity in intestinal-type and diffuse-type gastric carcinoma. Digestive Diseases and Sciences, 1997, 42(3): 576-579.

[4] Cheng H, Leblond C P. Origin differentiation and renewal of the four main epithelial cell types in the mouse small intestine. I. Columnar cell. Americal Journal of Anatomy, 1974, 141(4): 461-480.

[5] Clevers H, Nusse R. Wnt/b-Catenin Signaling and Disease. Cell, 2012, 149(10): 1192-1205.

[6] 王猛, 侯永清, 丁斌鹰, 刘玉兰, 朱惠玲, 胡泉舟, 肖华丽. 精胺对断奶仔猪小肠组织形态的影响. 动物营养学报, 2007, 19(4): 366-371.

Wang M, Hou Y Q, Ding B Y, Liu Y L, Zhu H L, Hu Q Z, Xiao H L. Effect of spermine on histological morphology and function of small intestine in weaned piglets. Chinese Journal of Animal Nutrition, 2007, 19(4): 366-371. (in Chinese)

[7] 何余湧, 邹伟, 王仁华, 刘晓兰, 陆伟. 饲料中添加精胺对哺乳仔猪血液生化指标及胃肠道发育的影响研究. 饲料工业, 2012, 33(9): 14-16.

He Y Y, Zou W, Wang R H, Liu X L, Lu W. Adding spermine on lactation piglet feed blood biochemical indexes and the influence of gastrointestinal development research. Feed Industry,2012, 33(9): 14-16. (in Chinese)

[8] Löser C, Eise A l, Harms, Fölsch U R. Dietary polyamines are essential D luminal growth foctors for small intestinal and colonic mucosal growth and development. Gut, 1999, 44: 12-16.

[9] Nick E. Flynn Æ Jared G. BirdÆ Aaron S. Guthrie. Glucocorticoid regulation of amino acid and polyamine metabolism in the small intestine. Amino Acids, 2009, 37(1): 123-129.

[10] 廖启顺, 苏子峰, 程志斌, 姚俊. 口服精胺对哺乳仔猪小肠生长的影响. 饲料博览•技术版, 2010(11): 5-7.

Liao Q S, Su Z F, Cheng Z B, Yao J. Effect on weaning piglets intestine by oral of spermine. Feed Expo Technology, 2010(11): 5-7. (in Chinese)

[11] Kim S W, Mateo R D, Yin Y L, Wu G Y. Function amino acid and fatty acids for enhancing production performance of sows and piglets. Asian-Australiasian Journal of Animal Sciences, 2007, 20(2): 295-306.

[12] Wu G Y, Bazer F W, Davis T A, Jaeger L A, Johnson G A, Kim S W, Knab D A, Meininger C J, Spencer T E, Yin Y L. Important roles for the arginine family of amino acids in swine nutrition and production. Livestock Science, 2007, 112: 8-22.

[13] Wu G, Collins J K, Perkins-Veazie P, Siddiq M, Dolan K D, Kelly K A, Heaps C L, Meininger C J. Dietary supplementation with watermelon pomace juice enhances arginine availability and ameliorates the metabolic syndrome in Zucker diabetic fatty rats. The Journal of Nutrition, 2007, 137(12): 2680- 2685.

[14] Brunton J A, Bertolo R F, Pencharz P B, Ball R O. Proline ameliorates arginine deficiency during enteral but not parenteral feeding in neonatal piglets. The American Journal of Physioloy, 1999, 277(2): 223-231.

[15] Urschel K L, Evans A R, Wilkinson C W, Pencharz P B, Ball R O. Infusion of glucagon-like peptide 2 with an arginine deficient diet increases endogenous arginine synthesis from proline in parenterally- fed neonatal piglets. The Journal of Nutrition, 2007, 137(3): 601-606.

[16] Louvard D, Kedinger M, Hauri H P. The differentiating intestinal epithelial cell: establishment and maintenance of functions through interactions between cellular structures. Annual Review of Cell Biology, 1992, 8: 157-195.

[17] Fan M Z, Matthews J C, Etienne N M, Stoll B, Lackeyram D, Burrin D G. Expression of apical membrane L-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2004, 287(2): 385-398.

[18] Yang C, Albin D M, Wang Z, Stoll B, Lackeyram D, Swanson K C, Yin Y, Tappenden K A, Mine Y, Yada R Y, Burrin D G, Fan M Z. Apical Na-D-glucose cotransporter 1 (SGLT1) activity and protein abundance are expressed along the jejunal crypt-villus axis in the neonatal pig. American Journal of Physiology Gastrointestinal and Liver Physiology, 2011, 300(1): 60-70.

[19] 张翥. 腐胺对断奶仔猪的营养生理作用及对后续生长性能的影响[D]. 重庆: 西南大学, 2009: 11-26.

Zhang Z. Effects of dietary putrescine addition on nutritional physiology of weanling pigs and their subsequent growth performance[D]. Chongqing: Southwest University, 2009: 11-26. (in Chinese)

[20] Wu G Y, Nick E F, Darrell A K. Enhanced intestinal synthesis of polyamines from proline in cortisol-treated piglets. American Journal of Physiology-Endocrinology and Metabolism, 2000, 279(2): 395-402.

[21] Grant A L, Thomas J W, King K J, Liesman J S. Effects of dietary amines on small intestinal variables in neonatal pigs fed soy protein isolate. Journal of Animal Science, 1990, 68(11): 363-371.

[22] Osipova L A, Nemli? N I, Gridina N Ia, Shliakhovenko V A. Effect of inhibitors of polyamine biosynthesis and od exogenous polyamines on mitogenic effect of epidermal growth factor in primary hepatocyte. Biulleten Eksperimental Biologii Meditsiny, 1989, 107(3): 339-342.

[23] Biol N, Garagba M C, Greco S, George P, Hugueny I, Louisot P. Polyamine participation in the maturation of glycoprotein fucosylation, but not sialylation, in rat small intestine. Pediatric Research, 2002, 51: 625-634.

[24] Bardo´ cz S, Grant G, Brown D S, Pusztai A. Putrescine as a source of instant energy in the small intestine of the rat. Gut, 1998, 42: 24-32.

[25] Bardocz S, Duguid T J, Brown D S, Grant G, Pusztai A, White A, Ralph A. The importance of dietary polyamines in cell regeneration and growth. British Journal of Nutrition, 1995, 73: 819-847.

[26] Loser C, Eisel A, Harms D, Folsch U R. Dietary polyamines are essential luminal growth factors for small intestinal and colonic mucosal growth and development. Gut, 1999, 44: 12-16.

[27] Grant A L, Thomas J W, King K J, Liesman J S. Effects of dietary amines on small intestinal variables in neonatal pigs fed soy protein isolate. Journal of Animal Science, 1990, 68: 363-371.

[28] Sabater-Molina M, Larque E, Torrella F, Ma J P, Lozano T, Mun A, Zamora S. Effects of dietary polyamines at physiologic doses in early-weaned piglets. Nutrition, 2009, 25(9): 940-946.

[29] Reya T, Clevers H. Wnt signaling in stem cells and cancer. Nature, 2005, 434(7035): 843-850.

[30] Pan W, Jia Y, Wang J, Tao D, Gan X, Tsiokas L, Jing N, Wu D, Li L. Beta-catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factorsinP19 cells. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(48): 17378-17383.

[31] Chen G, Jiang Q, You Z, Yao J, Mou L, Lin X, Shen X, You T, Lin Q, Wen J, Lin L. Regulation of GSK-3 beta in the proliferation and apoptosis of human thyrocytes investigate dusing a GSK-3 beta- targeting RNAi adenovirus expression vector: involvement the Wnt/beta-catenin pathway. Molecular Biology Reports, 2010, 37(6): 2773-2779.

[32] 关华, 郑加盟, 陈宗正, 宋子仪, 杨浩, 杨公社. 猪sFRP4基因的表达规律及抑制剂SP600125对前体脂肪细胞分化的影响. 中国生物化学与分子生物学报, 2012, 28(5): 455-463.

Guan H, Zheng J M, Chen Z Z, Song Z Y, Yang H, Yang G S. Expression of porcine 4 and its effect on differentiation of preadipocytes via inhibitor SP600125. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(5): 455-463. (in Chinese)

[33] Suzuki S, Sembon S, Iwamoto M, Fuchimoto D, Onishi A. Identification of genes downregulated during differentiation of porcine mesenteric. Journal of Animal Science, 2008, 86(12): 3367-3376.

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Impact of Putrescine and Proline on Suckling Piglet Jejunum Villus - crypt Axis Epithelial Polyamine Metabolism and Wnt Signal Pathway

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