Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (14): 2816-2825.doi: 10.3864/j.issn.0578-1752.2017.14.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Expression Patterns and Correlation of Wnts and TET1 Genes in Early Fetal Tissues of Dazu Black Goat

TAN Qiang, LUO NanJian, ZHANG YanLi, AN BingXing，CHEN QiuYang, ZHAO YongJu   

  1. College of Animal Science and Technology, Southwest University/Chongqing Key Laboratory of Forage & Herbivore / Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Chongqing 400715
  • Received:2016-09-01 Online:2017-07-16 Published:2017-07-16

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Abstract: 【Objective】The aim of the research was to quantitate the relative expression level of TET1, Wnt and DKK genes during early fetus development in goat, analyze the correlation between TET1 and Wnts genes in further. 【Method】Twelve healthy Dazu black goats copulated with the same ram by wild pairing while these goats with spontaneous estrus. Caesarean section was used to obtain fetus that pregnancy on days 20, 25, 30, 60 and 90, respectively, the growth indexes of the fetus (fetal weight, fetal length) were tested, and the tissue samples of 60d and 90d (fetal heart, liver, lung, kidney, brain and skin) were collected. Real-time (RT-PCR) was used to detect the relative expression level of TET1 gene, DKK family genes (DKK1, DKK2, DKK3) and Wnt family genes (Wnt2, Wnt2b, Wnt4, Wnt5a, Wnt5b, Wnt7b, Wnt16). SPSS software was used to analyze the correlation between TET1 and Wnt signaling pathway genes (P＜0.05) at different stages of fetal development. 【Result】 According to the statistics, there were significant changes in fetal development in early pregnancy after 60d. The results showed that the expression of TET1 gene increased with the development of gestational days. All Wnt family genes were detected in the fetus (Wnt2, -2b, -4, -5a, -5b, -7b, -16). Among these changes of fetal development, Wnt2 and Wnt7b expression increase with fetal development gradually; Wnt2b, Wnt5a, Wnt5b, and Wnt7b genes has a significant highly expression on pregnancy 30d (P＜0.05); the expression of Wnt4 was significantly in fetus on pregnancy 20d (P＜0.05); Wnt16 gene was significantly in fetus on pregnancy 25d (P＜0.05). The expression level of DKK family genes showed that DKK1 was detected in the early pregnancy of fetal development, and DKK2/3 performed a low expression level in the early pregnancy, whereas increased in later stage. The expression level of TET1 in liver, lung, kidney and brain in 90d fetus was higher than 60d fetal tissue, there was a significantly expression in liver (P＜0.05). Interestingly, Wnt2 had a highly significant expression level in all tissues of fetus on pregnancy 60 and 90d, especially in lung (P＜0.01); Wnt16 had a highly expression in fetal skin (P＜0.05); Wnt5a and Wnt7b had a significantly expression level in kidney (P＜0.05), other Wnt genes of Wnt family were detected in fetal tissues as well. Correlation analysis showed that the fetal growth indexes (fetal weight, body length) were positively correlated to changes in the expression of TET1 (P＜0.01); TET1 in early fetal development and the expression of Wnt2, Wnt7b and Wnt16 gene showed a positive correlation, and negatively correlated with Wnt2b, Wnt4, Wnt5a and Wnt5b, which was negatively correlated with Wnt5b (P＜0.05), and significantly and positively correlated with Wnt7b (P＜0.01). There was also a correlation between Wnt pathway genes, Wnt2 and Wnt4 showed a significant negative correlation (P＜0.01). Wnt2 and Wnt7b, Wnt2b and Wnt5a, Wnt5b, Wnt5a and Wnt5b showed a significant positive correlation (P＜0.01). Wnt4 was positively correlated with Wnt5a (P＜0.05). 【Conclusion】 The dynamic expression patterns of TET1 and Wnt genes in early fetal development of goat, and correlation of these genes were obtained from this study which fill the blank of those genes in this research field. The article revealed TET1 and Wnts regulate the embryonic development and the formation of tissues by a dynamic pattern. TET1appeared a positively correlation with some Wnt genes while negatively with others. It is also showed interactive in Wnt signaling pathway genes. The data obtained from the research will provide a reference for molecular mechanism research of TET1 and Wnts.

Key words: goat fetus, TET1, Wnt signaling pathway, expression pattern, correlation

[1]    ITO S, D'ALESSIO A C, TARANOVA O V, HONG K, SOWERS L CZHANG Y. Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification. Nature, 2010, 466(7310):1129-1151.
[2]    TAHILIANI M, KOH K P, SHEN Y H, PASTOR W A, BANDUKWALA H, BRUDNO Y, AGARWAL S, IYER L M, LIU D R, ARAVIND LRAO A. Conversion of 5-Methylcytosine to 5- Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1. Science, 2009, 324(5929):930-935.
[3]    FRAUER C, ROTTACH A, MEILINGER D, BULTMANN S, FELLINGER K, HASENODER S, WANG M X, QIN W H, SODING J, SPADA FLEONHARDT H. Different binding properties and function of CXXC zinc finger domains in dnmt1 and tet1. PLoS One, 2011, 6(2): e166217.
[4]    HAYASHI K, BURGHARDT R C, BAZER F WSPENCER T E. WNTs in the ovine uterus: Potential regulation of periimplantation ovine conceptus development. Endocrinology, 2007, 148(7): 3496-3506.
[5]    KIM H, JANG W Y, KANG M C, JEONG J, CHOI M, SUNG Y, PARK S, KWON W, JANG S, KIM M O, KIM S HRYOO Z Y. TET1 contributes to neurogenesis onset time during fetal brain development in mice. Biochemical and Biophysical Research Communications, 2016, 471(4):437-443.
[6]    KANG J, LIENHARDA M, PASTOR W A, CHAWLA A, NOVOTNY M, TSAGARATOU A, LASKEN R S, THOMPSON E C, SURANI M A, KORALOV S B, KALANTRY S, CHAVEZ LRAO A. Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(31):E4236-E4245.
[7]    YAMAGUCHI S, SHEN L, LIU Y T, SENDLER DZHANG Y. Role of Tet1 in erasure of genomic imprinting. Nature, 2013, 504(7480): 460-464.
[8]    NUSSE RVARMUS H E. Many tumors induced by the mouse mammary-tumor virus contain a provirus integrated in the same region of the host genome. Cell, 1982, 31(1):99-109.
[9]    DAS A, TANIGAWA S, KARNER C M, XIN M, LUM L, CHEN C, OLSON E N, PERANTONI A OCARROLL T J. Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation. Nature Cell Biology, 2013, 15(9):1035.
[10]   DASGUPTA RFUCHS E. Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. Development, 1999, 126(20):4557-4568.
[11]   GRIGORYAN T, WEND P, KLAUS ABIRCHMEIER W. Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of beta-catenin in mice. Genes Development, 2008, 22(17):2308-2341.
[12]   KIM B M, BUCHNER G, MILETICH I, SHARPE P TSHIVDASANI R A. The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling. Developmental Cell, 2005, 8(4):611-622.
[13]   PINTO D, GREGORIEFF A, BEGTHEL HCLEVERS H. Canonical Wnt signals are essential for homeostasis of the intestinal epithelium. Genes & Development, 2003, 17(14):1709-1713.
[14]   KEMP C, WILLEMS E, ABDO S, LAMBIV LLEYNS L. Expression of all Wnt genes and their secreted antagonists during mouse blastocyst and postimplantation development. Developmental Dynamics, 2005, 233(3):1064-1075.
[15]   SONDEREGGER S, POLLHEIMER JKNOFLER M. Wnt Signalling in Implantation, Decidualisation and Placental Differentiation - Review. Placenta, 2010, 31(10):839-847.
[16]   LU W G, TU Z W, WANG S M, LU J H, WANG Q, WANG W X, WANG B Y, WANG H B, NI H MGUO Y. Spatiotemporal expression of Wnt signaling pathway components during bovine placental development. Theriogenology, 2013, 80(8):893-902.
[17]   MARIKAWA Y. Wnt/beta-catenin signaling and body plan formation in mouse embryos. Seminars in Cell & Developmental Biology, 2006, 17(2):175-184.
[18]   NIEHRS C. Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene, 2006, 25(57):7469-7481.
[19]   LIEVEN O, KNOBLOCH JRUTHER U. The regulation of Dkk1 expression during embryonic development. Developmental Biology, 2010, 340(2):256-268.
[20]   FOSSAT N, JONES V, KHOO P L, BOGANI D, HARDY A, STEINER K, MUKHOPADHYAY M, WESTPHAL H, NOLAN P M, ARKELL RTAM P P L. Stringent requirement of a proper level of canonical WNT signalling activity for head formation in mouse embryo. Development, 2011, 138(4):667-676.
[21]   NERI F, DETTORI D, INCARNATO D, KREPELOVA A, RAPELLI S, MALDOTTI M, PARLATO C, PALIOGIANNIS POLIVIERO S. TET1 is a tumour suppressor that inhibits colon cancer growth by derepressing inhibitors of the WNT pathway. Oncogene, 2015, 34(32):4168-4176.
[22]   LI X, YUE X J, PASTOR W A, LIN L Z, GEORGES R, CHAVEZ L, EVANS S MRAO A. Tet proteins influence the balance between neuroectodermal and mesodermal fate choice by inhibiting Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(51):E8267-E8276.
[23]   ISHIKAWA T, TAMAI Y, ZORN A M, YOSHIDA H, SELDIN M F, NISHIKAWA STAKETO M M.   Mouse Wnt receptor gene Fzd5 is essential for yolk sac and placental angiogenesis. Development, 2001, 128(1):25-33.
[24]   PARR B A, CORNISH V A, CYBULSKY M IMCMAHON A P. Wnt7b regulates placental development in mice. Developmental Biology, 2001, 237(2):324-332.
[25]   NUSSE R.Wnt signaling in disease and in development.Cell Research, 2005. 15(1):28-32.
[26]   ATLI M O, GUZELOGLU ADINC D A. Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy.Animal Reproduction Science, 2011, 125(1-4):94-102.
[27]   PFAFFL M W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 2001, 29(9):e45.
[28]   WU H, ZHANG Y. Reversing DNA methylation: mechanisms, genomics, and biological functions. Cell, 2014, 156(1-2):45-68.
[29]   MEISSNER A, MIKKELSEN T S, GU H C, WERNIG M, HANNA J, SIVACHENKO A, ZHANG X L, BERNSTEIN B E, NUSBAUM C, JAFFE D B, GNIRKE A, JAENISCH RLANDER E S. Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature, 2008, 454(7205):766-791.
[30]   CAO Z B, ZHOU N R, ZHANG Y, ZHANG Y L, WU R H, LI Y S, ZHANG Y HLI N. Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology, 2014, 81(3): 496-508.
[31]   MOHAMED O A, DUFORT DCLARKE H J. Expression and estradiol regulation of Wnt genes in the mouse blastocyst identify a candidate pathway for embryo-maternal signaling at implantation. Biology of Reproduction, 2004, 71(2):417-424.
[32]   REYA TCLEVERS H. Wnt signalling in stem cells and cancer. Nature, 2005, 434(7035):843-850.
[33] QUINN K E, ASHLEY A K, REYNOLDS L P, GRAZUL-BILSKA A TASHLEY R L. Activation of the CXCL12/CXCR4 signaling axis may drive vascularization of the ovine placenta. Domestic Animal Endocrinology, 2014, 47:11-21.
[34]   POULAIN MOBER E A. Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development, 2011, 138(16):3557-3568.
[35]   CAPRIOLI A, VILLASENOR A, WYLIE L A, BRAITSCH C, MARTY-SANTOS L, BARRY D, KARNER C M, FU S, MEADOWS S M, CARROLL T JCLEAVER O. Wnt4 is essential to normal mammalian lung development. Developmental Biology, 2015, 406(2): 222-234.
[36]   RAJAGOPAL J, CARROLL T J, GUSEH J S, BORES S A, BLANK L J, ANDERSON W J, YU J, ZHOU Q, MCMAHON A PMELTON D A. Wnt7b stimulates embryonic lung growth by coordinately increasing the replication of epithelium and mesenchyme. Development, 2008, 135(9):1625-1634.
[37]   LI C G, XIAO J, HORMI K, BOROK ZMINOO P. Wnt5a participates in distal lung morphogenesis. Developmental Biology, 2002, 248(1): 68-81.
[38]   GOSS A M, TIAN Y, TSUKIYAMA T, COHEN E D, ZHOU D, LU M M, YAMAGUCHI T PMORRISEY E E. Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. Developmental Cell, 2009, 17(2):290-298.
[39]   PIETILA I, ELLWANGER K, RAILO A, JOKELA T, BARRANTES I D, SHAN J D, NIEHRS CVAINIO S J. Secreted Wnt antagonist Dickkopf-1 controls kidney papilla development coordinated by Wnt-7b signalling. Developmental Biology, 2011, 353(1):50-60.
[40]   MERKEL C E, KARNER C MCARROLL T J. Molecular regulation of kidney development: is the answer blowing in the Wnt? Pediatric Nephrology, 2007, 22(11):1825-1838.
[41]   TEH M T, BLAYDON D, GHALI L R, BRIGGS V, EDMUNDS S, PANTAZI E, BARNES M R, LEIGH I M, KELSELL D PPHILPOTT M P. Role for WNT16B in human epidermal keratinocyte proliferation and differentiation . Journal of Cell Science, 2007, 120(Ptz):330-339.
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