过量表达蔗糖转运蛋白基因增强转基因小麦的耐旱性

doi:10.3864/j.issn.0578-1752.2015.08.02

Abstract

Abstract: 【Objective】 Drought is one of the most important constraints resulting in large yield losses and limiting the average yield increase of wheat in China. The aim of this study is to develop and select stable TaSUT1A transgenic wheat lines with resistance to drought.【Method】The open-reading-frame sequence of TaSUT1A was synthesized and used to construct the gene transformation vector pUBI::cas-TaSUT1A, in which TaSUT1A gene was driven by maize ubiquitin promoter and should be highly expressed in monocot plants. Particle bombardment method was used to introduce TaSUT1A into wheat cultivar Kenong 199. After bialaphos screening, DNA PCR and RT-PCR methods were used to detect the presence and transcript levels of TaSUT1A in the transgenic wheat plants of T₀-T₄ generations. Subsequently, the resistance to water stress of transgenic wheat plants and non-transgenic wheat Kenong 199 at young seedling-stage was evaluated. Further, their determination of physiologic index related to abiotic stress was finished and the potential of improving abiotic stress tolerance in plant was elucidated. 【Result】The results indicated that the introduced TaSUT1A gene was stably inherited, and 220% PEG treatment could significantly induce the expression of TaSUT1A in root and leaf tissues of three transgenic wheat lines. The average germination rate of transgenic wheat lines was 85.53%, which was more higher than those of non-transgenic controls, and the average celoeptile and primary root length significantly higher than the non-transgenic plants. The results showed that the transgenic wheat significantly enhanced seed germination in 20% PEG treatment, as indicated by enhancement of the growth of celoeptile and primary root. Further, three pure lines with higher TaSUT1A expression were selected to elucidate physiologic index related to abiotic stress. In detail, in 20% PEG treatment, the transgenic lines overexpressing TaSUT1A increased sucrose and total soluble sugar content in leaf and root, with the average increase value of 42.95% and 36.56% in leaf, and 58.01% and 43.01% in root respectively, which was significantly higher than the non-transgenic plants. After treatment under water stress condition, the MDA content of transgenic plants only increased by 6.12 nmol·g^-1FW, however, the MAD content of the non-transgenic plants increased by 19.05 nmol·g^-1FW. Meanwhile, the transgenic plant showed better SOD activity, with the average increase value of 44.7 U·g^-1FW, which was significantly higher than the non-transgenic plants. 【Conclusion】It was concluded that TaSUT1A plays an important role in response to drought stress in plants, overexpression of TaSUT1A can improve significantly tolerance to drought stress in transgenic wheat plants.

Key words: common wheat, sucrose transporter, transgenic, drought stress, soluble sugar

HU Meng-yun, LI Hui, PANG Jian-zhou, LIU Qian, ZHANG Ying-jun, SUN Li-jing. Overexpression of Sucrose Transporter (TaSUT1A) Improves Drought Tolerance in Transgenic Wheat[J].Scientia Agricultura Sinica, 2015, 48(8): 1473-1483.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.08.02

https://www.chinaagrisci.com/EN/Y2015/V48/I8/1473

References

[1] 山仑. 生物节水研究现状及展望//香山科学会议第267次学术讨论会集. 北京: 香山科技会议, 2005: 3-14.

Shan L. Advances in biological water-saving research: Challenge and perspectives//A summary of the Academic Conference on 267th XiangShan-Science Conferences. Beijing: XiangShan- Science Conferences, 2005: 3-14. (in Chinese)

[2] Zhang J X, Klueva N Y, Wang Z, Wu R, Ho T H D, Nguyen H T. Genetic engineering for abiotic stress resistance in crop plants. In Vitro Cellular & Developmental Biology Plant, 2000, 36(2): 108-114.

[3] Ashraf M, Athar H R, Harris P J C, Kwon T R. Some prospective strategies for improving crop salt tolerance. Advances in Agronomy, 2008, 97: 45-110.

[4] Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S, Mittler R. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiology, 2004, 134: 1683-1696.

[5] Gupta A K, Kaur N. Sugar signaling and gene expression in relation to carbohydrate metabolism under abiotic stresses in plants. Journal of Biosciences, 2005, 30: 761-776.

[6] Rosa M, Prado C, Podazza G, Interdonato R, Juan A G, Hilal M, Prado F E. Soluble sugars-Metabolism, sensing and abiotic stress: A complex network in the life of plants. Plant Signaling Behavior, 2009, 4(5): 388-393.

[7] Ivan C. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany, 2006, 57(3): 449-459.

[8] Rolland F. Sugar sensing and signalling in plants, conserved and novel mechanisms. Annual Review of Plant Biology, 2006, 57: 675-709.

[9] Silvaa E N, Luiz F S S, Viégasb R A, Silveiraa J A G. The role of organic and inorganic solutes in the osmotic adjustment of drought- stressed Jatropha curcas plants. Environmental and Experimental Botany, 2010, 69(3): 279-285.

[10] Sivakumar P, Sharmila P, Jain V, Saradhi P P. Sugars have potential to curtail oxygenase activity of Rubisco. Biochemical and Biophysical Research Communications, 2002, 298: 247-250.

[11] Wingenter K. Increased activity of the vacuolar monosaccharide transporter TMT1 alters cellular sugar partitioning, sugar signalling and seed yield in Arabidopsis. Plant Physiology, 2010, 13: 1104-1110.

[12] Yamada K, Osakabe Y, Mizoi J, Nakashima K, Fujita Y, Shinozaki K, Yamaguchi-Shinozaki K. Functional analysis of an Arabidopsis thaliana abiotic stress-inducible facilitated diffusion transporter for monosaccharides. Journal of Biological Chemistry, 2010, 285(2): 1138-1146.

[13] 马小龙, 刘颖慧, 袁祖丽, 石云素, 宋燕春, 王天宇, 黎裕. 玉米蔗糖转运蛋白基因ZmERD6 cDNAs的克隆与逆境条件下的表达. 作物学报, 2009, 35(8): 1410-1417.

Ma X L, Liu Y H, Yuan Z L, Shi Y S, Song Y C, Wang T Y, Li Y. Cloning of cDNAs for a novel sugar transporter gene, ZmERD6, from maize and its expression analysis under abiotic stresses. Acta Agronomica Sinica, 2009, 35(8): 1410-1417. (in Chinese)

[14] Aoki N, Scofield G, Wang X D, Patrick J, Offler C, Furbank R T. Expression and localisation analysis of the wheat sucrose transporter TaSUT1 in vegetative tissues. Planta, 2004, 219: 176-184.

[15] Aoki N, Scofield G N, Wang X D, Offler C E, Patrick J W, Furbank R T. Pathway of sugar transport in germinating wheat seeds. Plant Physiology, 2006, 141: 1255-1263.

[16] 梁晓芳, 于振文. 施钾时期对冬小麦旗叶光合特性和籽粒淀粉积累的影响. 应用生态学报, 2004, 15(8): 1349-1352.

Liang X F, Yu Z W. Effect of potassium application stage on photosynthetic characteristics of winter wheat flag leaves and on starch accumulation in wheat grains. Chinese Journal of Applied Ecology, 2004, 15(8): 1349-1352. (in Chinese)

[17] 周玉梅, 韩士杰, 张军辉, 邹春静, 王琛瑞. CO₂浓度升高对长白山三种树木幼苗叶碳水化合物和氮含量的影响. 应用生态学报, 2002 13(6): 663-666.

Zhou Y M, Han S J, Zhang J H, Zou C J, Wang S R. Effect of elevated CO₂ concentration on carbohydrate and nitrogen contents in seedlings foliage of three tree species in Changbai Mountain. Chinese Journal of Applied Ecology, 2002, 13(6): 663-666. (in Chinese)

[18] Wang R, Chen S, Ma H, Liu L, Li H, Weng H, Hao Z, Yang S. Genotypic differences in antioxidative stress and salt tolerance of three poplars under salt stress. Frontiers of Forestry in China, 2006, 1: 82-88.

[19] 赵世杰. 植物生理学实验指导. 北京: 中国农业科学技术出版社, 2004.

Zhao S J. Plant Physiology Experimental Guidance. Beijing: China Agricultural Science and Technology Press, 2004. (in Chinese)

[20] 兰巨生. 农作物综合抗旱性评价方法的研究. 西北农业学报, 1998, 7(3): 85-87.

Lan J S. The research of identification drought resistance and its evaluation method. Acta Agriculturae Boreali-Occidentalis Sinica, 1998, 7(3): 85-87. (in Chinese)

[21] Keunen E, Peshev D, Vangronsveld J, Ende W V D, Cuypers A. Plant sugars are crucial players in the oxidative challenge during abiotic stress: Extending the traditional concept. Plant Cell and Environment, 2013, 36: 1242-1255.

[22] Eveland A L, Jackson D P. Sugars, signalling, and plant development. Journal of Experimental Botany, 2012, 63: 3367-3377.

[23] 杨晓杰, 刘传亮, 张朝军, 武芝霞, 张雪妍, 刘坤, 房卫平, 李付广. 不同转化方法获得的转基因棉花外源基因拷贝数分析. 农业生物技术学报, 2011, 19(2): 221-229.

Yang X J, Liu C L, Zhang Z J, Wu Z X, Zhang X Y, Liu K, Fang W P, Li F G. Comparative analysis of exogenous gene copy numbers in transgenic cotton transformed by different methods. Journal of Agricultural Biotechnology, 2011, 19(2): 221-229. (in Chinese)

[24] 王玮, 邹琦. 胚芽鞘长度作为冬小麦抗旱性鉴定指标的研究. 作物学报, 1997, 23(4): 459-467.

Wang W, Zou Q. Studies on coleoptile length as criterion of appraising drought resistance in wheat. Acta Agronomica Sinica, 1997, 23(4): 459-467. (in Chinese)

[25] 吴永成, 周顺利, 王志敏, 赵长星. 冬小麦初生根系与抗旱性及产量关系的初步研究. 青岛农业大学学报: 自然科学版, 2008, 25(3): 168-170.

Wu Y C, Zhou S L, Wang Z M, Zhao C X. Preliminary study on the relationship between primary root system and drought resistance and yield of winter wheat. Journal of Qingdao Agricultural University: Natural Science, 2008, 25(3): 168-170. (in Chinese)

[26] Gill P K, Sharma A D, Singh P, Bhullar S S. Effect of various abiotic stresses on the growth soluble sugars and water relations of sorghum seedlings grown in light and darkness. Journal of Plant Physiology, 2001, 27: 72-84.

[27] Gibson S I. Control of plant development and gene expression by sugar signaling. Current Opinion Plant Biology, 2005, 8: 93-102.

[28] 贺鸿雁, 孙存华, 杜伟, 李扬. PEG6000胁迫对花生幼苗渗透调节物质的影响. 中国油料作物学报, 2006, 28(1): 76-78.

He H Y, Sun C H, Du W, Li Y. Effects of PEG6000 osmtic stress on osmolytes of peanut seedling. Chinese Journal of Oil Crop Sciences, 2006, 28(1): 76-78. (in Chinese)

[29] 胡梦芸, 李辉, 张颖君, 刘茜. 水分胁迫下葡萄糖对小麦幼苗光合作用和相关生理特性的影响. 作物学报, 2009, 35(4): 724-732.

Hu M Y, Li H, Zhang Y J, Liu Q. Photosynthesis and related physiological characteristics affected by exogenous glucose in wheat seedlings under water stress. Acta Agronomica Sinica, 2009, 35(4): 724-732. (in Chinese)

[30] Hu M Y, Shi Z G, Zhang Z B, Zhang Y J, Li H. Effects of exogenous glucose on seed germination and antioxidant capacity in wheat seedlings under salt stress. Plant Growth Regulation, 2012, 68: 177-188.

[31] Siringam K, Juntawong N, Chaum S, Boriboonkaset T, Kirdmanee C. Salt tolerance enhancement in indica rice (Oryza sativa L.) seedlings using exogenous sucrose supplementation. Plant Omics, 2012, 5(1): 52-59.

[32] 赵莹, 杨克军, 赵长江, 李佐同, 王玉凤, 付健, 郭亮, 李文胜. 外源糖调控玉米光合系统和活性氧代谢缓解盐胁迫. 中国农业科学, 2014, 47(20): 3962-3972.

Zhao Y, Yang K J, Zhao C J, Li Z T, Wang Y F, Fu J, Guo L, Li W S. Alleviation of the adverse effects of salt stress by regulating photosynthetic system and active oxygen metabolism in maize seedlings. Scientia Agricultura Sinica, 2014, 47(20): 3962-3972. (in Chinese)

[33] Bolouri-Moghaddam R M, Roy K L, Li X, Rolland F, Van den Ende W. Sugar signalling and antioxidant network connections in plant cells. The FEBS Journal, 2010, 277: 2022-2037.

[34] Garg A K, Kim J K, Owens T G, Ranwala A P, Do Choi Y, Kochian L V, Wu R J. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proceedings of the National Academy Sciences of the United States of America, 2002, 99: 15898-15903.

[35] Sulmon C, Gouesbet C, Amrani A E, Couée S. Sugar-induced tolerance to the herbicide atrazine in Arabidopsis seedlings involves activation of oxidative and xenobiotic stress responses. Plant Cell Reports, 2006, 25: 489-498.

[36] Price J, Laxmi A, Martin S K S, Jang J C. Global transcription pro?ling reveals multiple sugar signal transduction mechanisms in Arabidopsis. The Plant Cell, 2004, 16: 2128-2150.

Related Articles 15

[1]	HU Sheng,LI YangYang,TANG ZhangLin,LI JiaNa,QU CunMin,LIU LieZhao. Genome-Wide Association Analysis of the Changes in Oil Content and Protein Content Under Drought Stress in Brassica napus L. [J]. Scientia Agricultura Sinica, 2023, 56(1): 17-30.
[2]	TANG HuaPing,CHEN HuangXin,LI Cong,GOU LuLu,TAN Cui,MU Yang,TANG LiWei,LAN XiuJin,WEI YuMing,MA Jian. Unconditional and Conditional QTL Analysis of Wheat Spike Length in Common Wheat Based on 55K SNP Array [J]. Scientia Agricultura Sinica, 2022, 55(8): 1492-1502.
[3]	DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.
[4]	LI Ning,LIU Kun,LIU TongTong,SHI YuGang,WANG ShuGuang,YANG JinWen,SUN DaiZhen. Identification of Wheat Circular RNAs Responsive to Drought Stress [J]. Scientia Agricultura Sinica, 2022, 55(23): 4583-4599.
[5]	LIU Hao,PANG Jie,LI HuanHuan,QIANG XiaoMan,ZHANG YingYing,SONG JiaWen. Effects of Foliar-Spraying Selenium Coupled with Soil Moisture on the Yield and Quality of Tomato [J]. Scientia Agricultura Sinica, 2022, 55(22): 4433-4444.
[6]	LI Gang,BAI Yang,JIA ZiYing,MA ZhengYang,ZHANG XiangChi,LI ChunYan,LI Cheng. Phosphorus Altered the Response of Ionomics and Metabolomics to Drought Stress in Wheat Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(2): 280-294.
[7]	RU Chen,HU XiaoTao,LÜ MengWei,CHEN DianYu,WANG WenE,SONG TianYuan. Effects of Nitrogen on Nitrogen Accumulation and Distribution, Nitrogen Metabolizing Enzymes, Protein Content, and Water and Nitrogen Use Efficiency in Winter Wheat Under Heat and Drought Stress After Anthesis [J]. Scientia Agricultura Sinica, 2022, 55(17): 3303-3320.
[8]	MA XueMeng,YU ChengMin,SAI XiaoLing,LIU Zhen,SANG HaiYang,CUI BaiMing. PSORA: A Strategy Based on High-Throughput Sequence for Analysis of T-DNA Insertion Sites [J]. Scientia Agricultura Sinica, 2022, 55(15): 2875-2882.
[9]	MENG Yu,WEN PengFei,DING ZhiQiang,TIAN WenZhong,ZHANG XuePin,HE Li,DUAN JianZhao,LIU WanDai,FENG Wei. Identification and Evaluation of Drought Resistance of Wheat Varieties Based on Thermal Infrared Image [J]. Scientia Agricultura Sinica, 2022, 55(13): 2538-2551.
[10]	HE KeWei,CHEN JiaFa,ZHOU ZiJian,WU JianYu. Fusarium verticillioides Resistant Maize Inbred Line Development Using Host-Induced Gene Silencing Technology [J]. Scientia Agricultura Sinica, 2021, 54(9): 1835-1845.
[11]	XIAO Fang,LI Jun,WANG HaoQian,ZHAI ShanShan,CHEN ZiYan,GAO HongFei,LI YunJing,WU Gang,ZHANG XiuJie,WU YuHua. Establishment and Application of A Duplex ddPCR Method to Quantify the NK603/zSSIIb Copy Number Ratio in Transgenic Maize NK603 [J]. Scientia Agricultura Sinica, 2021, 54(22): 4728-4739.
[12]	ZHU FangFang,DONG YaHui,REN ZhenZhen,WANG ZhiYong,SU HuiHui,KU LiXia,CHEN YanHui. Over-expression of ZmIBH1-1 to Improve Drought Resistance in Maize Seedlings [J]. Scientia Agricultura Sinica, 2021, 54(21): 4500-4513.
[13]	JIN Rong,LIU Ming,ZHAO Peng,ZHANG QiangQiang,ZHANG AiJun,TANG ZhongHou. IbMKP6, A Mitogen-Activated Protein Kinase, Confers Low Temperature Tolerance in Sweetpotato [J]. Scientia Agricultura Sinica, 2021, 54(20): 4265-4273.
[14]	XUE RenFeng,FENG Ming,HUANG YuNing,Matthew BLAIR,Walter MESSIER,GE WeiDe. Effects of PvEG261 Gene on the Fusarium Wilt and Drought- Resistance in Common Bean [J]. Scientia Agricultura Sinica, 2021, 54(20): 4274-4285.
[15]	SONG ShaoZheng,YU KangYing,ZHANG Ting,LU Rui,PAN ShengQiang,CHENG Yong,ZHOU MingMing. Preparation and Expression of rhPA/GH Double Transgenic Rabbits [J]. Scientia Agricultura Sinica, 2021, 54(2): 412-421.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Overexpression of Sucrose Transporter (TaSUT1A) Improves Drought Tolerance in Transgenic Wheat

RichHTML

PDF