不同水平玉米赤霉烯酮对断奶仔猪血清代谢产物和肝肾组织病理学影响

doi:10.3864/j.issn.0578-1752.2014.18.019

Abstract

Abstract: 【Objective】Zearalenone (ZEA) is one of estrogenic mycotoxins produced mainly by Fusarium fungigrowing on grains and their derived products. The aims of the present study were to investigate the effect of ZEA on serum metabolite, histopathology of liver and kidney and liver ultrastructure in weaned piglets.【Method】A total of twenty healthy weaned gilts (Landrace × Yorkshire × Duroc) with an average body weight of (10.36 ± 1.21) kg (mean ± SD) were used in the study. Gilts were randomly allocated into 4 treatments according to body weight and fed individually in a metabolic cage for 18 days after 7-d adaptation. Piglets were fed a basal diet only (control) or basal diet supplemented with ZEA at a dietary concentration of 1.1, 2.0 or 3.2 mg·kg^-1. Piglets were fasted for 12 h at the end of the experimental period. After the collection of blood samples, piglets were immediately killed by electrocution and livers and kidneys were isolated. Samples of livers and kidneys from each pig were quickly collected, one portion was fixed in 10% buffered formalin for histopathology evaluation, the second cutted into 0.5 mm³ was fixed in 2.5% polyoxymethylene-glutaraldehyde for ultrastructure analysis. 【Result】Results showed that piglets fed diets containing 3.2 mg·kg^-1 ZEA had increased serum bilirubin and decreased serum levels of globulin and triglyceride compared with the control (P＜0.05). Increasing dietary ZEA linearly decreased serum levels of globulin and triglyceride (P＜0.05). Liver sections from piglets treated with 1.1, 2.0 and 3.2 mg·kg^-1 ZEA showed hepatocyte swelling and granular degeneration compared with control. Histological examination of kidney in piglets fed 2.0 and 3.2 mg·kg^-1 ZEA revealed the degeneration and swelling in renal tubular epithelial cells and smaller lumina, and some renal tubules were filled with a large number of transparent bottom or proteinuria. Cytophagosome and leucocyte neutrophil were observed in the 2.0 and 3.2 mg·kg^-1 ZEA treatments. 【Conclusion】The above findings indicated that ZEA at level of 2.0 mg·kg^-1 was sufficient to induce hepatorenal toxicity of piglets, which may have implications for human health and animals production consuming ZEA-contaminated food or feed.

Key words: piglets, zearalenone, serum metabolite, hepatorenal histopathology, ultrastructure

JIANG Shu-zhen, SUN Hua, HUANG Li-bo, YANG Zai-bin, WANG Shu-jing, LIU Fa-xiao, F. Chi. Effects of Zearalenone Contaminated Diets on Serum Metabolite and Histopathology of Liver and Kidney in Weaned Piglets[J].Scientia Agricultura Sinica, 2014, 47(18): 3708-3715.

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