美洲南瓜（Cucurbita pepo）种皮苯丙氨酸解氨酶基因克隆与表达分析

doi:10.3864/j.issn.0578-1752.2014.06.018

Abstract

Abstract: 【Objective】The aim of this study was to clone full-length cDNA of phenylalanine ammonia-lyase (PAL) gene (CP-PAL) in seed coat of the hulled Cucurbita pepo, analyze its expressions in seed coat development of the hulled and hull-less C. pepo, thus providing a theoretical support for revealing the mechanism of seed development and function of accumulation of lignin in seed coat development. 【Method】 The full sequence of CP-PAL was cloned by RT-PCR and RACE techniques. The bioinformatics method was used to analyze cDNA sequence and deduced amino acid sequence, and the real-time PCR and 2-△△Ct method were used to analyze the expression profile of PAL gene in the whole period of seed coat development. 【Result】 The full-length sequence of CP-PAL consists of 1 720 bp with an intact open reading frame of 1 359 bp, 5′UTR of 114 bp, 3′UTR of 236 bp, polyA of 11 bp, encoding a polypeptide of 452 amino acids. The molecular weight of CP-PAL was 48.86 kD, pI was 6.5, total number of atoms was 6 885 and the formula was C2158H3449N607O657S14. Homology analysis showed that the CP-PAL nucleotide sequences and deduced amino acids were highly homologous to that of PAL gene in Cucumis sativus. CP-PAL contained three functional domains of PAL-HAL, PLN02457, phe_am_lyase and the typical PAL enzyme active site sequence (GTITASGDLVPLSYIA), and it was one member of Lyase_I_Like superfamily. CP-PAL was most likely to be located in the cytoplasm and endoplasmic reticulum, without signal peptide and leader peptide. And it was non-transmembrane and soluble protein. Besides, CP-PAL included casein kinase II phosphorylation site for four, protein kinase C phosphorylation site for six, N-myristoylation site for twelve and N-glycosylation site for two. In addition, CP-PAL had eighteen serine phosphorylation sites, six threonine phosphorylation sites and five tyrosine phosphorylation sites. Random coil was the maximum structural part in the protein secondary structure of CP-PAL, alpha helix and extended strand dispersed in whole protein, N-terminal region was presented mainly in the form of random coil, and C-terminal domain was existed in the form of extended strand. The multiple sequence alignment based on the deduced amino acid sequences of CP-PAL and other PALs of 14 plants showed the functional region was conserved, differences in the N-end. Phylogenetic tree analysis indicated that CP-PAL was very closely related to PAL of C. sativus. The main structural element in CP-PAL protein tertiary structure was α-Helix, less in β-Turn and random coil. Real-time PCR analysis revealed that PAL gene in hull-less C. pepo showed an opposite tendency to hulled C. pepo. The expression of PAL gene in hulled C. pepo was increased, but the expression in hull-less C. pepo was decreased after 20d of self-pollination. The expression of PAL gene in hull-less C. pepo was lower than that in hulled C. pepo. 【Conclusion】The gene CP-PAL which related to lignin synthesis was firstly cloned and characterized in seed coat of hulled C. pepo. The results of study indicated that it may affect seed coat development of the naked kernel variety from C. pepo by regulating the synthesis of lignin.

Key words: Cucurbita pepo, seed coat, phenylalanine ammonia-lyase (PAL), gene cloning, expression analysis

LIU Jia-1, 2 , 3 , XU Bing-Liang-1, 2 , 3 , XUE Ying-Yu-1, 2 , 3 , ZHANG Shu-Wu-1, 2 , 3 , CHEN Rong-Xian-4. Cloning and Expression Analysis of PAL Gene in Seed Coat of Cucurbita pepo[J].Scientia Agricultura Sinica, 2014, 47(6): 1216-1226.

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References

[1]Kumar A, Ellis B E. The phenylalanine ammonia-lyase gene family in raspberry. structure, expression, and evolution. Plant Physiology, 2001, 127(1): 230-239.

[2]欧阳光察, 薛应龙. 植物苯丙烷代谢的生理意义及调控. 植物生理学通讯, 1988, 24(3): 9-16.

Ouyang G C, Xue Y L. Physiologieal role and regulation of phenylpropanoid metabolism in plant. Plant Physiology Communications, 1988, 24(3): 9-16. (in Chinese)

[3]江昌俊, 余有本. 苯丙氨酸解氨酶的研究进展. 安徽农业大学学报, 2001, 28(4): 425-430.

Jiang C J, Yu Y B. The research progress (review) of phenylalanine ammonia lyase. Journal of Anhui Agricultural University, 2001, 28(4): 425-430. (in Chinese)

[4]Teppner H. Cururbita pepo. (Cucurbitaceae)-history, seed coat types, thin coated seeds and their genetics. Phyton (Horn, Austria), 2000, 40(1): 1-42.

[5]薛应钰, 师桂英, 徐秉良, 陈荣贤. 裸仁美洲南瓜种皮性状遗传规律研究. 草业学报, 2011, 20(3): 205-210.

Xue Y Y, Shi G Y, Xu B L, Chen R X. A study on the genetic law of seed coat characters in hull-less Cucurbita pepo. Acta Prataculturae Sinica, 2011, 20(3): 205-210. (in Chinese)

[6]Loy J B. Morpho-physiological aspects of productivity and quality in squash and pumpkins (Cucurbita spp.). Critical Reviews in Plant Sciences, 2004, 23(4): 337-363.

[7]Bezold T N, Mathews D, Loy J B, Minocha S C. Molecular analysis of the hull-less seed trait in pumpkin: expression profiles of genes related to seed coat development. Seed Science Research, 2005, 15(3): 205-217.

[8]薛应钰, 师桂英, 徐秉良, 陈荣贤. 美洲南瓜(Cucurbita pepo)种皮发育形态观察及其相关酶活性测定. 草业学报, 2011, 20(2): 23-30.

Xue Y Y, Shi G Y, Xu B L, Chen R X. Studies on morphology of seed coat development and its related enzyme activity assay in Cucurbita pepo. Acta Prataculturae Sinica, 2011, 20(2): 23-30. (in Chinese)

[9]Koukol J, Conn E E. The metabolism of aromatic compounds in high plant. Ⅳ. Purification and properties of the phenylalanine deaminase of Herdeum vulagare. Journal of Biology and Chemistry, 1961, 236(10): 2692-2698.

[10]欧阳光察, 应初衍, 沃绍根, 薛应龙. 植物苯丙氨酸解氨酶的研究.Ⅵ.水稻、小麦PAL的纯化及基本特性. 植物生理学报, 1985, 11(2): 204-214.

Ouyang G C, Ying C Y, Wo S G, Xue Y L. Study on plant phenylalanine ammonia-lyase, VI. purification and some properties of PAL from etiolated seedlings of rice (Oryza sativa) and wheat (Triticum aestivum). Journal of Plant Physiology, 1985, 11(2): 204-214. (in Chinese)

[11]Nakashima J, Tatsuya A, Keiji T, Minoru F, Hiroshi S. Immunocytochemical localization of phenylalanine ammonia-lyase and cinnamyl alcohol dehydrogenase in differentiating tracheary elements derived from Zinnia mesophyll cells. Plant Cell Physiology, 1997, 38(2): 113-123.

[12]Santiago L J M, Louro R P, Oliveira D D. Compartmentation of phenolic compounds and phenylalanine ammonia-lyase in leaves of Phyllanthus tenellus Roxb. and their induction by copper sulphate. Annals of Botany, 2000, 86(5): 1023-1032.

[13]Whetten R W, Sederoff R R. Phenylalanine ammonial-lyase from Loblolly pine: Purification of the enzyme and isolation of complementary DNA clones. Plant Physiology, 1992, 98(1): 380-386.

[14]Cunha A. Purification, characterization and induction of L-Phenylalanine ammonial-lyase in Phaseolus rulgaris. European Journal of Biochemistry, 1988, 178(1): 243-248.

[15]张宽朝, 金青, 蔡永萍, 林毅. 苯丙氨酸解氨酶与其在重要次生代谢产物调控中的作用研究进展. 中国农学通报, 2008, 24(12): 59-62.

Zhang K C, Jin Q, Cai Y P, Lin Y. Research progress of PAL and its control function of important secondav metabolltes. Chinese Agricultural Science Bulletin, 2008, 24(12): 59-62. (in Chinese)

[16]程水源, 陈昆松, 刘卫红, 杜何为. 植物苯丙氨酸解氨酶基因的表达调控与研究展望. 果树学报, 2003, 20(5): 351-357.

Cheng S Y, Chen K S, Liu W H, Du H W. Regulation and expression of the PAL in plant and its outlook. Journal of Fruit Science, 2003, 20(5): 351-357. (in Chinese)

[17]赵宗方, 赵勇, 吴桂法. 果实花青素含量与PAL活性关系的研究. 园艺学报, 1994, 21(2): 199-200.

Zhao Z F, Zhao Y, Wu G F. Studies on relationship between Anthocyanidin and PAL. Acta Horticulturae Sinica, 1994, 21(2): 199-200. (in Chinese)

[18]刘金福, 李晓雁, 孟蕊. 苦荞发芽过程中促进黄酮合成的因素初探. 食品工业科技, 2006, 27(10): 106-108.

Liu J F, Li X Y, Meng R. A primary discussion on the factors on promoting the synthesis of flavonoids in tartary buckwheat during germination. Science and Technology of Food Industry, 2006, 27(10): 106-108. (in Chinese)

[19]房建军, 阙国宁, 韩一凡. 银杏细胞培养中影响黄酮积累量的几个因素. 林业科学研究, 2006, 19(1): 51-53.

Fang J J, Que G N, Han Y F. Several factors affecting accumulation of flavonoids in suspension-cultured cells of Ginkgo biloba. Forest Research, 2006, 19(1): 51-53. (in Chinese)

[20]张淑珍, 徐鹏飞, 吴俊江, 王萍, 陈维元, 陈晨, 王金生, 靳立梅, 于安亮, 张大勇, 李文滨. 大豆疫霉根腐病菌毒素处理抗感不同大豆品种后苯丙氨酸解氨酶活性的变化. 作物杂志, 2008, 1: 47-49.

Zhang S Z, Xu P F, Wu J J, Wang P, Chen W Y, Chen C, Wang J S, Jin L M, Yu A L, Zhang D Y, Li W B. Change of phenylalanine ammonia-lyase (PAL) activities among different resistance soybean varieties treated with pathotoxin from Phytophthora sojae. Crops, 2008, 1: 47-49. (in Chinese)

[21]冯洁, 陈其煐. 棉株体内几种生化物质与抗枯萎之间关系的初步研究. 植物病理学报, 1991, 21(4): 291-297.

Feng J, Chen Q Y. On the biochemical substances in cotton plants related to the resistance to Fusarium wilt. Acta Phytopathologica Sinica, 1991, 21(4): 291-297. (in Chinese)

[22]尚庆茂, 张志刚. 亚精胺对黄瓜幼苗灰霉病的诱抗作用. 应用生态学报, 2008, 19(4): 825-830.

Shang Q M, Zhang Z G. Roles of spermidine in induced resistance of cucumber seedlings to Botrytis cinerea Pers. Chinese Journal of Applied Ecology, 2008, 19(4): 825-830. (in Chinese)

[23]吴龙火, 李庆, 杨群芳, 王海建. 禾谷缢管蚜取食5种山羊草的诱导抗性. 中国农业科学, 2008, 41(1): 102-107.

Wu L H, Li Q, Yang Q F, Wang H J. Resistance induced by feeding Rhopalosiphum padi with 5 Aegilops Species. Scientia Agricultura Sinica, 2008, 41(1): 102-107. (in Chinese)

[24]程水源, 杜何为, 许锋, 陈昆松. 银杏苯丙氨酸解氨酶基因的克隆和序列分析. 林业科学研究, 2005, 18(5): 573-577.

Chen S Y, Du H W, Xu F, Chen K S. Molecular cloning of phenylalanine ammonia-lyase in Ginkgo biloba. Forest Research, 2005, 18(5): 573-577. (in Chinese)

[25]李潞滨, 刘蕾, 张淑萍, 何聪芬, 胡桃, 庄彩云. 4种竹子木质素合成酶PAL的基因克隆和序列分析. 科技导报, 2009, 27(22): 19-25.

Li L B, Liu L, Zhang S P, He C F, Hu T, Zhuang C Y. Gene cloning and sequence analysis of phenylalanine ammonia-Lyase in bamboo lignin synthase. Science and Technology Review, 2009, 27(22): 19-25. (in Chinese)

[26]Xu F, Deng G, Cheng S Y, Zhang W W, Huang X H, Li L L, Cheng H, Rong X F, Li J B. Molecular cloning, characterization and expression of the phenylalanine ammonia-Lyase gene from Juglans regia. Molecules, 2012, 17(7): 7810-7823.

[27]Gao J H, Zhang S W, Cai F, Zheng X J, Lin N, Qin X B, Ou Y C, Gu X P, Zhu X H, Xu Y, Chen F. Characterization and expression profile of a phenylalanine ammonia lyase gene from Jatropha curcas L.. Molecular Biology Reports, 2012, 39(4): 3443-3452.

[28]Gao Z M, Wang X C, Peng Z H, Zheng B, Liu Q. Characterization and primary functional analysis of phenylalanine ammonia-lyase gene from Phyllostachys edulis. Plant Cell and Reports, 2012, 31(7): 1345-1356.

[29]Shang Q M, Li L, Dong C J. Multiple tandem duplication of the phenylalanine ammonia-lyase genes in Cucumis sativus L.. Planta, 2012, 236(4): 1093-1105.

[30]高颖, 李梦竹, 冯紫洲, 佟欢, 李毅, 张继星. 蓖麻PAL基因片段的克隆与序列分析. 内蒙古民族大学学报: 自然科学版, 2013, 28(2): 179-182, 195.

Gao Y, Li M Z, Feng Z Z, Tong H, Li Y, Zhang J X. Molecular cloning and sequence analysis of phenylalanine ammonia-lyase gene fragment in Ricinus communis. Joumal of Inner Mongolia University for Nationalities: Natural Sciences, 2013, 28(2): 179-182, 195. (in Chinese)

[31]江汉民, 王楠, 赵换, 孙德岭, 宋文芹. 花椰菜苯丙氨酸解氨酶基因的克隆及黑腐病菌胁迫下的表达分析. 南开大学学报: 自然科学版, 2012, 45(4): 87-92, 98.

Jiang H M, Wang N, Zhao H, Sun D L, Song W Q. Cloning of phenylanlanine ammonia-lyase gene from cauliflower and its expression under Xcc Stress. Acta Scientiarum Naturalium Universitatis Nankaiensis: Natural Science, 2012, 45(4): 87-92, 98. (in Chinese)

[32]郑洁红, 邱栋梁. 龙眼叶片PAL基因的克隆与表达研究. 热带作物学报, 2012, 33(1): 79-83.

Zheng J H, Qiu D L. Cloning and expression study of PAL in Longan leaves. Chinese Journal of Tropical Crops, 2012, 33(1): 79-83. (in Chinese)

[33]张继栋, 曹锦萍, 乔爱民, 李红梅, 孙敏, 何生根. 香石竹DcPAL1基因的克隆及其原核表达. 西北植物学报, 2012, 32(4): 657-664.

Zhang J D, Cao J P, Qiao A M, Li H M, Sun M, He S G. Cloning and prokaryotic expression of carnation DcPAL1 gene. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(4): 657-664. (in Chinese)

[34]黄志伟, 郑亚凤, 许明, 程祖锌, 郑金贵. 籼稻苯丙氨酸解氨酶基因的克隆与原核表达. 江苏农业学报, 2012, 28(5): 1194-1196.

Huang Z W, Zheng Y F, Xu M, Cheng Z X, Zheng J G. Cloning and prokaryotic expression of phenylalanine ammonia-lyase cDNA from Oryza satival L. subsp. Indica. Jiangsu Journal of Agricultural Sciences, 2012, 28(5): 1194-1196. (in Chinese)

[35]许锋, 曹腾, 宁迎晶, 蒋丽阳, 张威威, 程水源. 夏枯草苯丙氨酸解氨酶基因的克隆与表达分析. 华北农学报, 2012, 27(1): 39-44.

Xu F, Cao T, Ning Y J, Jiang L Y, Zhang W W, Cheng S Y. Molecular cloning and expression analysis of a phenylalanne ammonial-lyase gene from Prunella vulgaris. Acta Agriculturae Boreali-Sinica, 2012, 27(1): 39-44. (in Chinese)

[36]叶小利, 李加纳, 唐章林, 谌利. 甘蓝型黑籽和黄籽油菜种子发育过程中种皮色泽差异研究Ⅰ. 花色素、苯丙氨酸和苯丙氨酸解氨酶的变化及相关性. 中国油料作物学报, 2001, 23(2): 14-18.

Ye X L, Li J N, Tang Z L, Chen L. Difference of seedcoat color between black - and yellow- seeded in B. napus with seed development Ⅰ. Changes of anthocyanin, phenylalanine and phenylalaine ammonia -lyase and their correlation analyses. Chinese Journal of Oil Crop Sciences, 2001, 23(2): 14-18. (in Chinese)

[37]梁颖, 李加纳. 甘蓝型油菜种皮色泽形成与相关酶及蛋白质含量的关系. 中国农业科学, 2004, 37(4): 522-527.

Liang Y, Li J N. Relationship of the color formation and related enzymes and protein content of the spermoderms in Brassica napus. Scientia Agricultura Sinica, 2004, 37(4): 522-527. (in Chinese)

[38]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method. Methods, 2001, 25(4): 402-408.

[39]姜铮, 王芳, 何湘, 刘大伟, 陈宣男, 赵红庆, 黄留玉, 袁静. 蛋白质磷酸化修饰的研究进展. 生物技术通讯, 2009, 20(2): 233-237.

Jiang Z, Wang F, He X, Liu D W, Chen X N, Zhao H Q, Huang L Y, Yuan J. Progress on protein/peptide phosphorylation. Letters in Biotechnology, 2009, 20(2): 233-237. (in Chinese)

[40]宋福南, 邢磊, 陈肃, 戴超, 刘雪梅. 白桦苯丙氨酸解氨酶(PAL)基因的分离及其表达. 东北林业大学学报, 2009, 37(12): 14-17.

Song F N, Xing L, Chen S, Dai C, Liu X M. Expression and isolation of phenylalanine ammonia-lyase (PAL) gene in Betula platyphylla. Journal of Northeast Forestry University, 2009, 37(12): 14-17. (in Chinese)

[41]王云梅. 橄榄叶黄酮合成相关基因cDNA的克隆[D]. 福州: 福建农林大学, 2009.

Wang Y M. Clong of cDNA encoding for associated gene of flavones biosynthesis in Chinese white olive (Canarium album raeusch. cv. Changying) leaves[D]. Fuzhou: Fujian Agriculture and Forestry University, 2009. (in Chinese)

[42]张鲁斌, 朱世江, 刘建双, 常金梅. 菜心苯丙氨酸解氨酶基因克隆与序列分析. 西北植物学报, 2010, 30(12): 2379-2384.

Zhang L B, Zhu S J, Liu J S, Chang J M. Cloning and sequence analysis of BcPAL in Tsai Tai (Brassica chinensis). Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(12): 2379-2384. (in Chinese)

[43]康晓慧, 雷桅, 张梅. 水稻苯丙氨酸解氨酶的生物信息学分析. 湖南师范大学自然科学学报, 2010, 33(4): 89-94.

Kang X H, Lei W, Zhang M. Bioinformatic analysis of phenylalanine ammonia-lyase gene in Oryza sativa. Journal of Natural Science of Hunan Normal University, 2010, 33(4): 89-94. (in Chinese)

[44]宋修鹏, 黄杏, 莫凤连, 田丹丹, 杨丽涛, 李杨瑞, 陈保善. 甘蔗苯丙氨酸解氨酶基因（PAL）的克隆和表达分析. 中国农业科学, 2013, 46(14): 2856-2868.

Song X P, Huang X, Mo F L, Tian D D, Yang L T, Li Y R, Chen B S. Cloning and expression analysis of sugarcane phenylalanine ammonia-lyase (PAL) gene. Scientia Agricultura Sinica, 2013, 46(14): 2856-2868. (in Chinese)

[45]Bate N J, Orr J, Ni W, Meromi A, Nadler-Hassar T, Doerner P W, Dixon R A, Lamb C J, Elkind Y. Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate-determining step in natural product synthesis. Proceedings of the National Academy of Science of the USA, 1994, 91(16): 7608-7612.

[46]Sewalt V J H, Ni W, Blount J W, Jung H G, Masoud S A, Howles P A, Lamb C, Dixon R A. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamate 4-hydroxylase. Plant Physiology, 1997, 115(1): 41-50.

[47]陈晓光, 石玉华, 王成雨, 尹燕枰, 宁堂原, 史春余, 李勇, 王振林. 氮肥和多效唑对小麦茎秆木质素合成的影响及其与抗倒伏性的关系. 中国农业科学, 2011, 44(17): 3529-3536.

Chen X G, Shi Y H, Wang C Y, Yin Y P, Ning T Y, Shi C Y, Li Y, Wang Z L. Effects of Nitrogen and PP333 application on the lignin synthesis of stem in relation to lodging resistance of wheat. Scientia Agricultura Sinica, 2011, 44(17): 3529-3536. (in Chinese)

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