Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4784-4790.doi: 10.3864/j.issn.0578-1752.2013.22.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Characterization and Expression Analysis of Goat Ovarian chi-miR-100f-5p

 NA  Ri-Su-12, LIU  Guang-1, XIANG  Yang-1, ZHAO  Hai-Lin-1, WU  Ting-1, ZHAO  Jin-Hong-2, LI  Fan-1, HUANG  Xuan-Yang-1, SUN  Xiao-Wei-1, ZHENG  Jiao-1, ZHANG  Jia-Hua-1   

  1. 1.College of Animal Science and Technology, Southwest University/Chongqing Key Laboratory of Forage and Herbivore/Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Chongqing 400715
    2.Chongqing Academy of Animal Husbandry, Chongqing 400716
  • Received:2013-01-25 Online:2013-11-15 Published:2013-04-23

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Abstract: 【Objective】 The relationship between the goat ovarian expressed miR-100 with its predicted target genes and goat ovarian function or follicular development was studied in order to provide data for goat breeding mechanism.【Method】 Goat ovarian expressed miR-100 was obtained by cloning and sequencing a small RNA library with pooled RNA from goat ovarian tissue. The expression profiling was detected by RT-qPCR. miR-100 target genes and functional classification were predicted by bioinformatics method.【Result】The chi-miR-100f-5p, at a length of 21 nt , was isolated from goat ovaries. The sequence is highly homologous with dan-miR-100, and has one loci in the genome negative (-) chain, which located in goats sequence CM000899.1: 33997894 to 33997914; The chi-miR-100f-5p was detected in goat ovarian, pituitary, heart and other tissues, showing chi-miR- 100f-5p was not an ovarian-specific miRNA. It was found that chi-miR-100f-5p expressed at higher level in heart and pituitary, secondly in ovary, and the expression of chi-miR-100f-5p in the liver, spleen, lung, and kidney was significantly lower than in ovary (P＜0.01). It was alsofound that chi-miR-100f-5p expressed at significantly higher level in single lambing Inner Mongolia cashmere goat ovary than the high fecundity Dazu black goat ovary (P＜0.01); The chi-miR-100f-5p was mainly targeted for integrin β1 subunit and pituitary-specific transcription factor 1 gene, and might be involved in the integrin signaling pathway, promoting the growth and differentiation of pituitary endocrine cells, and regulating the hypothalamic-pituitary-gonadal axis hormonessecretion.【Conclusion】The newly discovered chi-miR-100f-5p in goat ovarian may be an important candidate miRNA in regulation of breeding.

Key words: chi-miR-100f-5p , microRNA , ovary

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