Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4378-4387.doi: 10.3864/j.issn.0578-1752.2013.20.022

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effect of Heat Stress on the Intestinal Flora Structure and Alkaline Phosphatase Activities and mRNA Expression of Amino Acid Transporters of Layer

 LI  Yong-Zhu-1, CHEN  Chang-Xiu-1, Yongquan Cui2   

  1. 1.College of Life Science, Linyi University, Linyi 276005, Shandong, China
    2.Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
  • Received:2013-03-25 Online:2013-10-15 Published:2013-07-11

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Abstract: 【Objective】 The objective of this study is to reveal the influence mechanisms of heat stress affecting the intestinal flora structure of layer, the alkaline phosphatase activities of intestinal mucosa and the mRNA expression of amino acid transporters.【Method】A total of 96 Jining Bairi chicken aged at 16 weeks were chosen and randomly divided into control group (group I, (24±1)℃) and heat stress group ((38±1)℃). six replicates were desighed for each group with 8 individuals for each replicate, and the duration sustained for 14 days. PCR-DGGE and RT-PCR of 16S rDNA were used. The bacterial diversity of the contents in dodecadactylon, jejunum and ileum, alkaline phosphatase activities of intestinal mucosa and the mRNA expression of amino acid transporters e.g. rBAT, y+LAT1, and CATl were analyzed when the layers were under the heat stress on 2nd day (group II), 7th day (group III) and 14th day(group IV), respectively.【Result】The bacterial diversities of each intestinal segments showed relatively abundant from the 7th day after heat stress. On the 7th and 14th days, no sensitive lactobacillus in jejunum and ileum was detected, and so were the L. johnsonii, nonculturable bacteria and Bacteroides in ileum. While the nonculturable bacteria, ulcer Bacteroides, ulcer Bacteroides, and Shigella sonnei were detected at every phase of heat stress. The alkaline phosphatase activities of mucosal epithelial cells in jejunum and ileum decreased significantly compared to the control group (P＜0.05). The mRNA expressions of rBAT, y+LAT1 of the group III in jejunum and ileum were the lowest. The variation extent of the mRNA expression in jejunum at each phase of heat stress was the greatest (P＜0.05). The mRNA expressions of CAT1 in ileum of groups III and IV were affected more greatly by the heat stress compared to that of group I (P＜0.01).【Conclusion】The influence on the intestinal flora in jejunum and ileum by heat stress is more evidently and the intestinal function of digestion and absorption can be changed by the variation of the intestinal flora..

Key words: heat stress , laying hens , intestinal flora , alkaline phosphatase activity , amino acid transporters

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