代乳粉对早期断奶沂蒙黑山羊羔小肠发育、菌群多样性及葡萄糖转运载体基因表达的影响

doi:10.3864/j.issn.0578-1752.2018.11.016

Abstract

Abstract: 【Objective】 Thisstudy was conducted to evaluate the effects of the milk replacer on the intestinal development, the flora diversity and the relative expression of glucose transporter gene of Yimeng black goat lambs in order to provide theoretical basis for the cultivation of early stage Yimeng black goat lambs. 【Method】 A total of 36 twin born Yimeng black goat lambs were randomly divided into 2 groups, each with 6 replicates and each replicate 3 lambs. The lambs in the control group (group I) were fed with breast milk for 75 days, while the lambs in the experimental group (group II) were weaned on the 10th day and fed with milk replacer thereafter. The weight of duodenum, jejunum, ileum as well as live body of the lambs in the two groups were recorded on the 10, 15, 25, 45 and 75th day, and meanwhile, the small intestinal contents and mucous tissue samples were collected. The colonization of small intestinal flora and the expression of glucose transporter (SGLT-1, GLUT-2) gene mRNA were analyzed by the PCR-DGGE of 16S rDNA and real-time fluorescence quantitative PCR, respectively. 【Result】 The results showed that the average daily gain and the weight of jejunum and ileum of the lambs in group II on the 45th day were significantly higher than those in group I (P＜0.05), while on the 75th day, the average daily gain and ileum weight of the lambs in group II were highly significantly higher than those in group I (P＜0.01). The flora distribution difference between group I and group II was significant in the duodenum on the 45 and 75th day, in the jejunum on the 15th day and in the ileum on the 10th day. The flora diversity in the ileum was higher than that in the jejunum and duodenum, and the microbiota diversity in the jejunum was higher than that in the duodenum. The bacterium lacticum dissolving starch and prevotella were dominant bacteria in the whole intestinal tract of Group I and II. The number of probiotics in group I decreased with the growth of age, while an opposite dynamic was found in the group II. The expression of SGLT-1 and GLUT-2 genes in the group II was the lowest at the 10 d, but their expression increased on the 25th day, meanwhile the two genes took on different expression profiles in different intestinal segments. The fluctuation of expression level of SGLT-1 was the highest and lowest in the jejunum and duodenum, respectively, while that of GLUT-2 was the biggest and lowest in the duodenum and ileum, respectively.【Conclusion】 In conclusion, feeding milk replacer could promote early colonization of probiotics in the jejunum and ileum of early weaned Yimeng black goat lambs on the 25th day and the glucose absorption and transport could be changed by regulating glucose transporter gene expression, which further affected later development of intestinal tissue and the growth of the lambs.

Key words: milk replacer, intestinal flora, glucose transporter, Yimeng black goat

LI YongZhu,JIN TaiHua, HAN ZhaoQing, XIA ChunFeng, CHAO HongYu, ZHANG NaiFeng, WANG ShiQin, DIAO QiYu. Effects of the Milk Replacer on the Development of Intestine, the Flora Diversity and the Relative Expression of Glucose Transporter Gene of Early Weaned Yimeng Black Goat Lambs[J].Scientia Agricultura Sinica, 2018, 51(11): 2193-2205.

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Effects of the Milk Replacer on the Development of Intestine, the Flora Diversity and the Relative Expression of Glucose Transporter Gene of Early Weaned Yimeng Black Goat Lambs

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