Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3177-3184 .doi: 10.3864/j.issn.0578-1752.2011.15.013

• HORTICULTURE • Previous Articles     Next Articles

Effects of Phenolic Acids on Mitochondria and the Activity of Antioxidant Enzymes in Roots of Seedlings of Malus hupehensis Rehd.

ZHANG  Zhao-Bo, MAO  Zhi-Quan, ZHU  Shu-Hua   

  1. 1. 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室
    2. 山东农业大学化学与材料科学学院
  • Received:2010-12-13 Revised:2011-04-07 Online:2011-08-01 Published:2011-04-18

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Abstract: 【Objective】Experiments were conducted to study the effects of ρ-hydroxybenzoic acid, phloroglucinol, syringic acid, benzoic acid, caffeic acid and ferulic acid on the functions of mitochondria and the activities of antioxidant enzymes in roots of Malus hupehensis seedlings which were apples common stocks.【Method】 Malus hupehensis seedlings were planted in bowl and were teated with 50 mL of 0.8, 4.0, 20.0 mmol•L-1 of the six kinds of phenolic acids at 3-day intervals. Fresh weight, activities of superoxidase (SOD), catalase (CAT) and peroxidase (POD), the opening of mitochondrial permeability transition pores (MPTP), membrane fluidity and cytochrome c/a were determined. 【Result】 Treatments with 0.8, 4.0, 20.0 mmol•L-1 of six kinds of phenolic acids all inhibited the growth of Malus hupehensis seedlings, reduced the activities of SOD, POD, and CAT, increased the opening of MPTP, decreased membrane fluidity and Cyt c/a, and impaired mitochondria in a sense. Benzoic acid at 20.0 mmol•L-1 had more significant inhibitions than other treatments. 【Conclusion】 The six kinds of phenolic acids at 0.8, 4.0, 20.0 mmol•L-1 could inhibit the activities of antioxidant enzymes and mitochondrial functions, and the inhibition depended on dose of phenolic acids. At the same concentrations, benzoic acid has stronger inhibition than other five phenolic acids.

Key words: Malus hupehensis, phenolic acids, antioxidative enzyme, mitochondria

[1]张江红, 毛志泉, 王丽琴, 束怀瑞. 根皮苷对平邑甜茶幼苗生理特性的影响. 中国农业科学, 2007, 40(3): 492-498.

Zhang J H, Mao Z Q, Wang L Q, Shu H R. Effect of phloridzin on physiological characteristics of Malus hupehensis Rehd. seedlings. Scientia Agricultura Sinica, 2007, 40(3): 492-498. (in Chinese)

[2]谢洪刚, 李  坤. 果树连作障碍机理及控制途径. 辽宁农业职业技术学院学报, 2008, 10(4): 6-8.

Xie H G, Li K. Mechanism and ways of control on replant diseases of fruit trees. Journal of Liaoning Agricultural College, 2008, 10(4): 6-8. (in Chinese)

[3]Hiraddate S, Morita S, Furubayashi A, Fujii Y, Harada J. Plant growth inhibition by cis-cinnamoyl glucosides and cis-cinnamic acid. Journal of Chemical Ecology, 2005, 31(3): 591-601.

[4]Abenavoli M R, de Santis C, Sidari M, Sorgonà A, Badiani M, Cacco G. Influence of coumarin on the net nitrate uptake in durum wheat. New Phytologist, 2001, 3: 619-627.

[5]白  朴, 张国平, 王元辉. 作物生化它感现象及其应用展望. 上海农业学报，2002, 18(4): 21-25.

Bai P, Zhang G P, Wang Y H. The allelopathy of crop and prospects for the application in agriculture. Acta Agriculturae Shanghai, 2002, 18(4): 21-25. (in Chinese)

[6]Inderjit K M, Duke S O. Ecophysiological aspects of allelopathy. Planta, 2003, 217: 529-539.

[7]王  闯, 徐公义, 葛长城, 毛志泉. 酚酸类物质和植物连作障碍的研究进展. 北方园艺, 2009(3):134-137.

Wang C, Xu G Y, Ge C C, Mao Z Q. Progress on the phenolic acid substances and plant soil sickness. Northern Horticulture, 2009(3):134-137. (in Chinese)

[8]Schieber A, Hilt P, Streker P, Endreb H, Rentschler C, Carle R. A new process for the combined recovery of pectin and phenolic compounds from apple pomace. Innovative Food Science and Emerging Technologies, 2003(4): 99-107.

[9]张江红, 张殿生, 毛志泉, 李中勇, 赵明新. 苹果砧木幼苗根系分泌物的分离与鉴定. 河北农业大学学报, 2009, 32(4): 29-32.

Zhang J H, Zhang D S, Mao Z Q, Li Z Y, Zhao M X. Isolation and identification of root exudates in different kinds of apple rootstock seedlings. Journal of Agricultural University of Hebei, 2009, 32(4): 29-32. (in Chinese)

[10]高相彬, 赵凤霞, 沈 向, 胡艳丽, 郝云红, 杨树泉, 苏立涛, 毛志泉. 肉桂酸对平邑甜茶幼苗根系呼吸速率及相关酶活性的影响. 中国农业科学, 2009, 42(12): 4308- 4314.

Gao X B, Zhao F X, Shen X, Hu Y L, Hao Y H, Yang S Q, Su L T, Mao Z Q. Effects of cinnamon acid on respiratory rate and its related enzymes activity in roots of seedlings of Malus hupehensis Rehd. Scientia Agricultura Sinica, 2009, 42(12): 4308-4314. (in Chinese)

[11]高相彬, 胡艳丽, 赵凤霞, 毛志泉, 沈 向, 杨树泉, 苏立涛. 外源苯甲酸对平邑甜茶幼苗根系膜脂过氧化的影响. 林业科学, 2009, 45(12): 129-134.

Gao X B, Hu Y L, Zhao F X, Mao Z Q, Shen X, Yang S Q, Su L T. Effects of benzoic acid application on membrance lipid peroxidation in roots of Malus hupehensis seedlings. Scientia Silvae Sinica, 2009, 45(12): 129-134. (in Chinese)

[12]李培栋, 王兴祥, 李奕林, 王宏伟, 梁飞燕, 戴传超. 连作花生土壤中酚酸类物质的检测及其对花生的化感作用. 生态学报, 2010, 30(8): 2128-2134.

Li P D, Wang X X, Li Y L, Wang H W, Liang F Y, Dai C C. The contents of phenolic acids in continuous cropping peanut and their allelopathy. Acta Ecologica Sinica, 2010, 30(8): 2128-2134. (in Chinese)

[13]马云华, 王秀峰, 魏  珉, 亓延凤, 李天来. 黄瓜连作土壤酚酸类物质积累对土壤微生物和酶活性的影响. 应用生态学报, 2005, 16(11): 2149-2153.

Ma Y H, Wang X F, Wei M, Qi Y F, Li T L. Accumulation of phenolic acids in continuously cropped cucumber soil and their effects on soil microbes and enzyme activities. Chinese Journal of Applied Ecology, 2005, 16(11): 2149-2153. (in Chinese).

[14]白  茹. 苹果连作障碍中自毒作用的研究[D]. 陕西杨凌: 西北农林科技大学, 2009.

Bai R. Autointoxication in apple replant disease[D]. Yangling, Shaanxi: Northwest A＆F University, 2009. (in Chinese)

[15]乔  林, 陈德昌, 景炳文, 李红江, 杨兴易. 大黄对烫伤后大鼠肠粘膜上皮细胞线粒体的影响.中华急诊医学杂志, 2002, 11(1): 9-11.

Qiao L, Chen D C, Jing B W, Li H J, Yang X Y. The protective effects of rhubarb on mitochondria function of intestinal mucosa in burned rats. Journal of Emergency Medicine, 2002, 11(1): 9-11. (in Chinese)

[16]Peeler T C, Stephenson M B, Einspahr K J, Thomnson G A Jr. Lipid characterization of an enriched plasma membrane fraction of Dunaliella salina grown in media of varying salinity. Plant Physiology, 1989, 89(3): 970-976.

[17]孟凡娟, 黄凤兰. 盐胁迫下刺槐叶片线粒体及超微结构的变化. 经济林研究, 2010, 28(2): 18-23.

Meng F J, Huang F L. Changes of function and ultrastructure of mitochondria in Robinia pseudoacacia leaves under salt stress. Nonwood Forest Research, 2010, 28(2): 18-23. (in Chinese)

[18]马怀宇, 吕德国, 杨洪强. NaCl胁迫下平邑甜茶根系线粒体特性和细胞死亡特征. 植物生态学报, 2010, 34(12): 1448-1453.

Ma H Y, Lü D G, Yang H Q. Characteristics of mitochondria and cell death in roots of Malus hupehensis var. pingyiensis under NaCl stress. Chinese Journal of Plant Ecology, 2010, 34(12): 1448-1453. (in Chinese)

[19]赵世杰, 史国安, 董新纯. 植物生理学实验指导. 北京: 中国农业科技出版社, 2002.

Zhao S J, Shi G A, Dong X C. Direction for Plant Physiology Experiments. Beijing: China Agricultural Science and Technology Press, 2002. (in Chinese)

[20]Omran R G. Peroxide levels and the activities of catalase, peroxidase, and indoleacetic acid oxidase during and after chilling cucumber seedlings. Plant Physiology, 1980, 65(2): 407-408.

[21]杨玖英, 谭艳平,  夏春皎, 朱英国, 刘学群. 红莲型细胞质雄性不育性与线粒体渗透性转换.武汉植物学研究, 2004, 22(5): 385-390.

Yang J Y, Tan Y P, Xia C J, Zhu Y G, Liu X Q. Honglian cytoplasmic male sterility in relation to its mitochondrial permeability transition. Journal of Wuhan Botanical Research, 2004, 22(5): 385-390. (in Chinese)

[22]钱琼秋, 宰文姗, 何 勇, 王永传, 朱祝军. 外源硅和辅酶Q10对盐胁迫下黄瓜根系线粒体的保护作用. 中国农业科学, 2006, 39(6): 1208-1214.

Qian Q Q, Zai W S, He Y, Wang Y C, Zhu Z J. Protection of exogenous silicon and CoQ10 on mitochondria in cucumber (Cucumis sativus L.) roots under salt stress. Scientia Agricultura Sinica, 2006, 39(6): 1208-1214. (in Chinese)

[23]姚婷婷, 朱丽琴, 杨  双, 周  杰, 朱树华. 一氧化氮对采后李果实线粒体膜氧化损伤的影响. 中国农业科学, 2010, 43(13): 2767-2774.

Yao T T, Zhu L Q, Yang S, Zhou J, Zhu S H. Effect of NO on oxidative damage to mitochondrial membrane in harvested plum fruit. Scientia Agricultura Sinica, 2010, 43(13): 2767-2774. (in Chinese)

[24]Tonshin A A, Saprunova V B, Solodovnikova I M, Bakeeva L E, Yaguzhinsky L S. Functional activity and ultra structure of mitochondrial isolated from myocardial apoptotic tissue. Biochemistry (Moscow), 2003, 68: 875-881.

[25]Bai R, Ma F W, Liang D, Zhao X. Phthalic acid induces oxidative stress and alters the activity of some antioxidant enzymes in roots Malus prunifolia. Journal of Chemical Ecology, 2009, 35(4): 488-494.

[26]杨树泉, 沈  向, 毛志泉, 尹承苗, 王  峰, 王青青. 环渤海湾苹果产区老果园与连作园土壤线虫群落特征.生态学报,2010, 30(16): 4445-4451.

Yang S Q, Shen X, Mao Z Q, Yin C M, Wang F, Wang Q Q. Characterization of nematode communities in the soil of long-standing versus replanted apple orchards surrounding Bohai Gulf. Acta Ecologica Sinica, 2010, 30(16): 4445-4451. (in Chinese).

[27]尹永强, 胡建斌, 邓明军. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展. 中国农学通报, 2007, 23(1): 105-110.

Yin Y Q, Hu J B, Deng M J. Latest development of antioxidant system and responses to stress in plant leaves. Chinese Agricultural Science Bulletin, 2007, 23(1): 105-110. (in Chinese)

[28]王才斌, 吴正锋, 成 波, 郑亚萍, 万书波, 郭 峰, 陈殿绪. 连作对花生光合特性和活性氧代谢的影响. 作物学报, 2007, 33(8): 1304-1309.

Wang C B, Wu Z F, Cheng B, Zheng Y P, Wan S B, Guo F, Chen D X. Effect of continuous cropping on photosynthesis and metabolism of reactive oxygen in peanut. Acta Agronomica Sinica, 2007, 33(8): 1304-1309. (in Chinese)

[29]Halestrap A P. What is the mitochondrial permeability transition  pore. Journal of Molecular and Cellular Cardiology, 2009, 46(6): 821-831.

[30]陈  沁, 章文华, 刘友良. 外源H2O2 和•OH 对大麦幼苗根系线粒体膜脂和流动性的伤害. 西北植物学报, 2001, 21(3): 468-473.

Chen Q, Zhang W H, Liu Y L. Injury effect of exogenous H2O2 and •OH on membrane lipids and fluidity of the roots of barley. Acta Botanica Boreali-Occidentalia Sinica, 2001, 21(3): 468-473. (in Chinese)
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