Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (15): 3108-3114.doi: 10.3864/j.issn.0578-1752.2012.15.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of Phloridzin on the Tricarboxylic Acid Cycle Enzymes of Roots of Malus hupehensis Rehd.

 WANG  Qing-Qing, HU  Yan-Li, ZHOU  Hui, ZHAN  Xing, MAO  Zhi-Quan, ZHU  Shu-Hua   

  1. 1.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安271018
    2.山东农业大学化学与材料科学学院，山东泰安271018
  • Received:2012-02-02 Online:2012-08-01 Published:2012-05-21

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Abstract: 【Objective】A pot experiment was conducted to study the effects of phloridzin on the tricarboxylic acid cycle (TCA) of roots of Malus hupehensis Rehd. which is used widely as apple common stocks. The mechanisms were discussed so as to provide a basis for further study on the cause of apple continuous cropping diseases. 【Method】 Phloridzin of 4 mmol•L-1 and KMnO4 of 1 mmol•L-1 were used in the pretreatment. Malus hupehensis Rehd. were planted in pots and treated with phloridzin of 4 mmol•L-1 (T1) and phloridzin of 4 mmol•L-1 added with KMnO4 of 1 mmol•L-1 (T2). The content of phloridzin in the soil and respiratory rate of roots were determined. Activities of enzymes related to TCA including citrate synthase (CS), aconitase, isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate thiokinase (SCS), succinate dehydrogenase (SDH), malic dehydrogenase (MDH), fumarase (FUM) and pyruvate dehydrogenase (PDH) were also determined.【Result】Treatments with T1 inhibited the respiratory rate of roots of Malus hupehensis Rehd., decreased obviously enzyme activities for the most part including CS, aconitase, ICDH, SCS, SDH, FUM, MDH only except for α-KGDH and PDH that their enzymes activities were improved. However, treatments with T2 had a critical difference from T1, it significantly enhanced the activities of CS, aconitase and MDH even over the control level, increased obviously the activity of SCS close to the control level, and to some degree improved the activities of ICDH, SDH and FUM. Besides, the activities of α-KGDH and PDH of T2 treatments were assayed to be a lower level than T1 but higher than the control level. Furthermore, the content of phloridzin in soil of T2 was less than T1.【Conclusion】The results showed that phloridzin at the concentration of 4 mmol•L-1 could inhibit the respiratory rate and the enzyme activities (7 of 9) related to TCA of roots of Malus hupehensis Rehd. with the treatments time following. The treatment of 1 mmol•L-1 KMnO4 could ease the above-mentioned adverse impact.

Key words: Malus hupehensis Rehd., phenolic acids, phloridzin, tricarboxylic acid cycle, KMnO4, continuous cropping

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